¹HNMR studies on human plasma lipids from newborn infants, healthy adults, and adults with tumors

Abstract

The 1H NMR spectra of the lipid region of human plasma from healthy adults, neonates, and patients with malignant and nonmalignant tumors have been recorded on a JNM-GX400 FT spectrometer operating at 399.6 MHz for protons. The chemical shifts of methylene and methyl groups of plasma lipids were measured with respect to the higher field component of the methyl proton resonance of the lactate molecule. The results show that there are changes in the chemical shifts of the methylene proton resonances among the plasma from healthy adults, adults with tumors, and neonates. The shifts observed in the case of cancer patients and neonates are in the direction opposite to the shift measured from the plasma of healthy adults. Thus, the observed changes cannot be explained by the activity in the cell proliferation of tissues which is high in the cases of both healthy neonates and patients with malignant tumors, but they most probably reflect the different lipoprotein compositions of neonates, healthy adults, and adults with tumors.

Proton nuclear magnetic resonance lineshape studies on human blood plasma lipids from newborn infants, healthy adults, and adults with tumors

“…After the original suggestion made by Fossel et al ( 1 ) to use the mean linewidths of the methyl and methylene regions in the proton NMR spectra to indicate cancer in a patient, many investigations have been published on the subject (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(21)(22)(23). The effects of experimental conditions on the results have been extensively studied.…”