2023
DOI: 10.1002/mrc.5409
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13C‐depleted algae as food: Permitting background free in‐vivo nuclear magnetic resonance of Daphnia magna at natural abundance

William W. Wolff,
Jacob Pellizzari,
Ronald Soong
et al.
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(4 citation statements)
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“…Without oxygen, the organisms started to die within the first 1 h and all were dead by 7.5 h. However, with the oxygen turned on and creating circulating flow all the Daphnia survived for 24 h in triplicate trials. Note, 24 h were chosen as nearly all studies to date using 2D NMR for understanding pathways have been conducted over this period. ,,, For readers interested in studies longer than 24 h please see Figure S6, which demonstrates the growth of algae over 4 days, as both a demonstration and proof-of-concept. The system was also tested with lab air instead of oxygen, however a tiny fraction of the organisms at the 24 h point (Figure S7) died.…”
Section: Resultsmentioning
confidence: 99%
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“…Without oxygen, the organisms started to die within the first 1 h and all were dead by 7.5 h. However, with the oxygen turned on and creating circulating flow all the Daphnia survived for 24 h in triplicate trials. Note, 24 h were chosen as nearly all studies to date using 2D NMR for understanding pathways have been conducted over this period. ,,, For readers interested in studies longer than 24 h please see Figure S6, which demonstrates the growth of algae over 4 days, as both a demonstration and proof-of-concept. The system was also tested with lab air instead of oxygen, however a tiny fraction of the organisms at the 24 h point (Figure S7) died.…”
Section: Resultsmentioning
confidence: 99%
“…Note, 24 h were chosen as nearly all studies to date using 2D NMR for understanding pathways have been conducted over this period. 5,11,13,23 For readers interested in studies longer than 24 h please see Figure S6, which demonstrates the growth of algae over 4 days, as both a demonstration and proof-of-concept. The system was also tested with lab air instead of oxygen, however a tiny fraction of the organisms at the 24 h point (Figure S7) died.…”
Section: Development and Application Of The Oxygenationmentioning
confidence: 99%
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