1984
DOI: 10.1104/pp.74.1.43
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[14C]Sucrose Uptake and Labeling of Starch in Developing Grains of Normal and segl Barley

Abstract: Previous work showed that the segl mutant of barley (Hordeum vulgare cv Betzes) did not differ from normal Betzes in plant growth, photosynthesis, or fertility, but it produced only shrunken seeds regardless of pollen source. To determine whether defects in sucrose uptake or starch synthesis resulted in the shrunken condition, developing grains of Betzes and segl were cultured in I'4Cisucrose solutions after slicing transversely to expose the endosperm cavity and free space. In both young grains (before genoty… Show more

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Cited by 17 publications
(7 citation statements)
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“…To study grain growth with respect to oxygen limitation, the Suc uptake rate was fixed at 8 mmol g 21 dry weight h 21 , a value derived from experimental reports (Felker et al, 1984), and the oxygen uptake rate was varied from completely anaerobic to fully aerobic conditions (Fig. 2).…”
Section: Grain Growthmentioning
confidence: 99%
See 1 more Smart Citation
“…To study grain growth with respect to oxygen limitation, the Suc uptake rate was fixed at 8 mmol g 21 dry weight h 21 , a value derived from experimental reports (Felker et al, 1984), and the oxygen uptake rate was varied from completely anaerobic to fully aerobic conditions (Fig. 2).…”
Section: Grain Growthmentioning
confidence: 99%
“…The exchange flux for Suc was constrained between 0 and 8 mmol g 21 dry weight h 21 , a value taken from experimental reports (Felker et al, 1984). In these [ 14 C]Suc-labeling experiments, the Suc uptake in developing grain of barley was measured to be 5 and 8 mmol g 21 dry weight h 21 for 17 and 5 d after anthesis, respectively.…”
Section: Simulation Conditionsmentioning
confidence: 99%
“…According to the definition by Felker et al (1984), the wild-type and csa stems containing internodes I to IV, the flag leaf, and flowers were placed and incubated in water containing 1 mCi [fructose-U-14 C]sucrose (21.8 GBq mmol 21 in 9:1 ethanol:water; MP Biochemicals), and the internode IV was submerged in the water directly. We detected the amount of isotope using the middle regions of internodes I, II, and III.…”
Section: Radiolabelingmentioning
confidence: 99%
“…At the latter stages, cavities or localized necrosis were formed in the endosperm tissue of shriveled kernels, and aleurone cells were disrupted in most of the area surrounding the cavities (12,16). A premature cessation ofgrain-filling was found to be a common phenomenon of shrunken seed types in cereals (8,12,16). What causes the necrosis and the premature cessation of normal tissue development is of importance in discerning the mechanism of cereal seed shriveling, particularly in early generation hybrids of inter-and intra-specific or intergeneric crosses.…”
mentioning
confidence: 99%