<sup>15</sup>N-markiertes Hefeproteinhydrolysat als Tracer für Stoffwechseluntersuchungen

Krawielitzki, K.; Schadereit, R.; Völker, Traude; Herrmann, U.; Wutzke, K. D.

doi:10.1080/10256018708623794

Isotopenpraxis Isotopes in Environmental and Health Studies

1987

DOI: 10.1080/10256018708623794

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¹⁵N-markiertes Hefeproteinhydrolysat als Tracer für Stoffwechseluntersuchungen

K. Krawielitzki

R. Schadereit

Traude Völker

et al.

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Cited by 3 publications

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Development and application of¹⁵N-tracer substances for measuring the whole-body protein turnover rates in the human, especially in neonates: a review

Wutzke

2012

Isotopes in Environmental and Health Studies

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Our research group of the Children's Hospital of the University of Rostock (Rostock group) has long-time experience in (15)N-labelling and in using yeast protein and its hydrolysates for tracer kinetic studies to evaluate parameters of the whole-body protein metabolism in premature infants. The particular advantage of applying an economically convenient, highly (15)N-enriched, and completely labelled yeast protein for evaluating protein turnover rates is the fact that the (15)N dose is spread among all proteinogenic amino acids. The absorption has been improved by hydrolysing [(15)N]yeast protein with thermitase into a mixture of amino acids, dipeptides and tripeptides so that faecal analysis becomes unnecessary when determining turnover rates. The review shows that, in contrast to the application of single (15)N-labelled amino acids with resulting overestimation of protein turnover rates, the (15)N-labelled yeast protein thermitase hydrolysate represents the amino acid metabolism more closely without causing amino acid imbalances. The (15)N-labelled yeast protein thermitase hydrolysate leads to the estimation of reliable protein turnover rates, particularly in premature infants.

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Development and application of¹⁵N-tracer substances for measuring the whole-body protein turnover rates in the human, especially in neonates: a review

Wutzke

2012

Isotopes in Environmental and Health Studies

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The Influence of Feeding Frequency on Nitrogen Turnover and Whole Body Protein Synthesis in Adult Rats (Estimation using [¹⁴C]- and [¹⁵N]- Labelled Leucine)

Krawielitzki¹,

Schreurs

Kreienbring³

et al. 1997

Isotopes in Environmental and Health Studies

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Bestimmung von kinetischen Parametern des Proteinmetabolismus bei wachsenden Ratten in Verbindung mit Energieumsatzmessungen. 1. Mitteilung. Bestimmung von kinetischen Parametern des Proteinmetabolismus in Abhängigkeit von der Lebendmasse und dem Proteingehalt des Futters

Krawielitzki

Schadereit

Klein

et al. 1991

Archiv für Tierernaehrung

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The digestibility, the N balance, the rate of protein synthesis and other parameters, characterising the protein metabolism in dependence on live weight, protein- and energy supply are estimated on Wistar rats (4-5 animals/group). These experiments were done in 5 alternating consecutive growth and energy maintenance periods at 4 different levels of protein (6, 10, 17, 26% CP) during the live weight period of 70 to 230 g. The rate of protein synthesis was calculated from the course of renale 15N excretion by means of the end product method after giving a single dose of a mixture of 17 15N labelled amino acids. N deposition, rate of protein synthesis and flux rate increased with the protein level of the ration. During maintenance these data were much lower, but showed the same dependence on the protein level. The absolute protein synthesis (g/d) increased up to the live weight of 130... 180 g and decreased afterwards according to the age. The reutilization rate varied between 44 and 87% and decreased with increasing dietary protein level by 27% and during proceeding age by 8 ... 12%. In contrast to the absolute metabolism rates (g/d) the fractional rates (%/d) clearly decreased with the age of the animals. The stimulation of these rates by the dietary protein level resembled that for the absolute rates of synthesis. The protein deposition showed the typical course of a growing curve according to the N intake and the protein synthesis showed practically the same course but on a higher level. The break down remained constantly (approximately 140 mg N/d) up to an N intake of about 360 mg and afterwards it increased too.

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¹⁵N-markiertes Hefeproteinhydrolysat als Tracer für Stoffwechseluntersuchungen

Cited by 3 publications

References 13 publications

Development and application of¹⁵N-tracer substances for measuring the whole-body protein turnover rates in the human, especially in neonates: a review

Development and application of¹⁵N-tracer substances for measuring the whole-body protein turnover rates in the human, especially in neonates: a review

The Influence of Feeding Frequency on Nitrogen Turnover and Whole Body Protein Synthesis in Adult Rats (Estimation using [¹⁴C]- and [¹⁵N]- Labelled Leucine)

Bestimmung von kinetischen Parametern des Proteinmetabolismus bei wachsenden Ratten in Verbindung mit Energieumsatzmessungen. 1. Mitteilung. Bestimmung von kinetischen Parametern des Proteinmetabolismus in Abhängigkeit von der Lebendmasse und dem Proteingehalt des Futters

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15N-markiertes Hefeproteinhydrolysat als Tracer für Stoffwechseluntersuchungen

Cited by 3 publications

References 13 publications

Development and application of15N-tracer substances for measuring the whole-body protein turnover rates in the human, especially in neonates: a review

Development and application of15N-tracer substances for measuring the whole-body protein turnover rates in the human, especially in neonates: a review

The Influence of Feeding Frequency on Nitrogen Turnover and Whole Body Protein Synthesis in Adult Rats (Estimation using [14C]- and [15N]- Labelled Leucine)

Bestimmung von kinetischen Parametern des Proteinmetabolismus bei wachsenden Ratten in Verbindung mit Energieumsatzmessungen. 1. Mitteilung. Bestimmung von kinetischen Parametern des Proteinmetabolismus in Abhängigkeit von der Lebendmasse und dem Proteingehalt des Futters

Contact Info

Product

Resources

About

¹⁵N-markiertes Hefeproteinhydrolysat als Tracer für Stoffwechseluntersuchungen

Development and application of¹⁵N-tracer substances for measuring the whole-body protein turnover rates in the human, especially in neonates: a review

Development and application of¹⁵N-tracer substances for measuring the whole-body protein turnover rates in the human, especially in neonates: a review

The Influence of Feeding Frequency on Nitrogen Turnover and Whole Body Protein Synthesis in Adult Rats (Estimation using [¹⁴C]- and [¹⁵N]- Labelled Leucine)