[<sup>3</sup>H]‐adenine nucleotide and [<sup>3</sup>H]‐noradrenaline release evoked by electrical field stimulation, perivascular nerve stimulation and nicotine from the taenia of the guinea‐pig caecum

Kuchii, Masato; Miyahara, J. T.; Shibata, Shoji

doi:10.1111/j.1476-5381.1973.tb08371.x

Cited by 31 publications

(13 citation statements)

References 9 publications

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“…In our previous experiments we showed that although electrical stimulation of the taenia caecum is associated with efflux of both [3 H] -nucleotides and [3 HI-NA, only that for noradrenaline was proportional to the intensity of stimulation and relaxation (Kuchii et al, 1973a). These data further demonstrated a dissociation of the inhibitory effect of nicotine and of transmural stimulation from [3 H I -nucleotide release in the cold stored as well as reserpine-treated taenia.…”

Section: Discussionsupporting

confidence: 48%

“…With electrical stimulation, the amplitude of the inhibitory response was dependent on the frequency, as well as on the intensity of stimulation (Kuchii, et al, 1973a by 0.65 ± 0.1 and 0.68 ± 0.08 ,uCi g-1 min-' (s.e. mean, n = 10), respectively, over the background level.…”

Section: Electrical Stimulationmentioning

confidence: 99%

“…Previously it was shown that the relaxation evoked by stimulation of the perivascular nerves was associated with a proportionate increase in the release of [3 HI-NA (Kuchii et al, 1973a).…”

Section: Perivascular Nerve Stimulationmentioning

confidence: 99%

“…Moreover, since the intestinal smooth muscle preferentially accumulates adenine nucleoside and the relaxation in response to electrical stimulation was associated with the release of adenine nucleotide, these investigators proposed that the neurohumoral transmitter of the non-adrenergic neurone may be ATP or a closely related nucleotide (Burnstock, 1972). Hattori, Kurhashi, Mori & Shibata (1972) and Kuchii, Miyahara & Shibata (1973a, b) Radioisotopic studies of noradrenaline and adenosine release were carried out as described by Kuchii et al (1973a).…”

Section: Introductionmentioning

confidence: 99%

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EFFECT OF 6‐HYDROXYDOPAMINE ON [³H]‐ADENINE NUCLEOTIDE AND [³H]‐NORADRENALINE RELEASE FROM THE GUINEA‐PIG TAENIA CAECUM EVOKED BY ELECTRICAL FIELD STIMULATION, NICOTINE AND PERIVASCULAR NERVE STIMULATION

Kuchii

Miyahara

Shibata

1974

British J Pharmacology

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The effects of pretreatment of guinea‐pigs with 6‐hydroxydopamine (6‐OHDA) on the release of [3H]‐noradrenaline and [3H]‐adenine nucleotide following electrical field, nicotine and perivascular nerve stimulation of guinea‐pig taenia caecum were studied. High frequency electrical field stimulation (15,30 Hz) released both [3H]‐noradrenaline and [3H]‐adenine nucleotide but low frequency (0.5, 5 Hz) stimulation, producing comparable muscle relaxation led only to [3H]‐noradrenaline release. 6‐OHDA (50–200 mg/kg) pretreatment inhibited the muscle relaxation and, [3H]‐noradrenaline release with electrical stimulation or with nicotine in isolated taenia but did not affect the release of [3H]‐nucleotide. A low dose of 6‐OHDA (50 mg/kg), completely inhibited the muscle relaxation and [3H]‐noradrenaline release elicited by perivascular nerve stimulation. Both tissue noradrenaline content and [3H]‐noradrenaline uptake were decreased to the same extent by low as well as high doses of 6‐OHDA: noradrenaline content was reduced to 20% and uptake to 30% of the control value. Catecholamine fluorescence disappeared from tissue layers of the taenia after treatment with a high dose of 6‐OHDA. In these experiments the inhibitory action of electrical stimulation and nicotine on the taenia can be correlated better with noradrenaline than with nucleotide release.

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Section: Discussionsupporting

confidence: 48%

Section: Electrical Stimulationmentioning

confidence: 99%

“…Previously it was shown that the relaxation evoked by stimulation of the perivascular nerves was associated with a proportionate increase in the release of [3 HI-NA (Kuchii et al, 1973a).…”

Section: Perivascular Nerve Stimulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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EFFECT OF 6‐HYDROXYDOPAMINE ON [³H]‐ADENINE NUCLEOTIDE AND [³H]‐NORADRENALINE RELEASE FROM THE GUINEA‐PIG TAENIA CAECUM EVOKED BY ELECTRICAL FIELD STIMULATION, NICOTINE AND PERIVASCULAR NERVE STIMULATION

Kuchii

Miyahara

Shibata

1974

British J Pharmacology

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“…It has been reported that, after adenosine has been taken up, it is converted to adenosine nucleotides (Jacob & Berne, 1960;Su & Bevan, 1970;Kuchii, Miyahara & Shibata, 1973;Hulme & Weston, 1974) or degraded to inosine or hypoxanthine (Koss et al, 1962;Bunag et al, 1964;Deuticke & Gerlach, 1966). In view of these reports and the present observations, dilazep may not affect the uptake of adenosine, but rather its subsequent phosphorylation to nucleotides by adenosine kinase or its degradation in the cell.…”

Section: Discussionmentioning

confidence: 99%

Potentiation by Dilazep of the Negative Inotropic Effect of Adenosine on Guinea‐pig Atria

Fujita

Ishida

Izumi

et al. 1980

British J Pharmacology

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IDilazep, a coronary dilator, has been reported to potentiate the negative inotropic and negative chronotropic responses of guinea-pig atria to adenosine. Studies were made on the mechanism of the potentiating action of dilazep with special reference to the degradation and uptake of adenosine. 2 The negative inotropic actions of adenosine and adenine nucleotides, such as ATP, ADP, AMP and cyclic AMP, on guinea-pig atria were selectively and dose-dependently augmented by dilazep at concentrations insufficient to produce any effect alone (0.01 to 1 tM). 4 Adenosine (10 pM to 10 mM) was degraded to inosine and hypoxanthine during incubation with atrial tissue; dilazep (0.1 to 10 pM) retarded the disappearance of adenosine and the formation of inosine and hypoxanthine. 5 These results suggest that dilazep potentiates the negative inotropic effect of adenosine on guineapig atria by preventing both its accumulation by atrial tissue and degradation by deaminase.

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Comparative studies of purinergic nerves

Burnstock

1975

J. Exp. Zool.

129

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Purinergic nerves supply the gastrointestinal tract of vertebrates, including fish, amphibians, reptiles and birds, as well as mammals. Their cell bodies are located in Auerbach's plexus and their axons extend in an anal direction before innervating mainly the circular muscle coat. In the stomach they are controlled by preganglionic cholinergic fibres of parasympathetic origin. They are involved in "receptive relaxation" of the stomach, "descending inhibition" in peristalsis and reflex relaxation of oesophageal and internal anal sphincters. The terminal varicosities of purinergic nerves are characterised by a predominance of "large opaque vesicles," which can be distinguished from the "large granular vesicles" found in small numbers in both adrenergic and cholinergic nerves. Stimulation of purinergic nerves with single pulses produces hyperpolarisations of up to 25 mV (inhibitory junction potentials) in smooth muscle cells. These potentials are unaffected by atropine, adrenergic neuron blocking agents or sympathetic denervation, but are abolished by tetrodotoxin. The "rebound contraction" which characteristically follows cessation of purinergic nerve stimulation is probably due to prostaglandin. Evidence that ATP is the transmitter released from purinergic nerves includes: (1) synthesis and storage of ATP in nerves; (2) release of ATP from the nerves when they are stimulated; (3) exogenously applied ATP mimicking the action of nerve-released transmitter, both producing a specific increase in K+ conductance; (4) the presence of Mg-activated ATPase, 5'-nucleotidase and adenosine deaminase, enzymes which inactivate ATP; (5) drugs (including quinidine, some 2-substituted imidazolines, 2-2'pyridylisatogen and dipyridamole) which produce similar blocking or potentiating effects on the response to exogenously applied ATP and nerve stimulation. Speculations are made about the evolution and development of the nervous system, including the possibility that purinergic nerves are a primitive nerve type.

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[³H]‐adenine nucleotide and [³H]‐noradrenaline release evoked by electrical field stimulation, perivascular nerve stimulation and nicotine from the taenia of the guinea‐pig caecum

Cited by 31 publications

References 9 publications

EFFECT OF 6‐HYDROXYDOPAMINE ON [³H]‐ADENINE NUCLEOTIDE AND [³H]‐NORADRENALINE RELEASE FROM THE GUINEA‐PIG TAENIA CAECUM EVOKED BY ELECTRICAL FIELD STIMULATION, NICOTINE AND PERIVASCULAR NERVE STIMULATION

EFFECT OF 6‐HYDROXYDOPAMINE ON [³H]‐ADENINE NUCLEOTIDE AND [³H]‐NORADRENALINE RELEASE FROM THE GUINEA‐PIG TAENIA CAECUM EVOKED BY ELECTRICAL FIELD STIMULATION, NICOTINE AND PERIVASCULAR NERVE STIMULATION

Potentiation by Dilazep of the Negative Inotropic Effect of Adenosine on Guinea‐pig Atria

Comparative studies of purinergic nerves

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[3H]‐adenine nucleotide and [3H]‐noradrenaline release evoked by electrical field stimulation, perivascular nerve stimulation and nicotine from the taenia of the guinea‐pig caecum

Cited by 31 publications

References 9 publications

EFFECT OF 6‐HYDROXYDOPAMINE ON [3H]‐ADENINE NUCLEOTIDE AND [3H]‐NORADRENALINE RELEASE FROM THE GUINEA‐PIG TAENIA CAECUM EVOKED BY ELECTRICAL FIELD STIMULATION, NICOTINE AND PERIVASCULAR NERVE STIMULATION

EFFECT OF 6‐HYDROXYDOPAMINE ON [3H]‐ADENINE NUCLEOTIDE AND [3H]‐NORADRENALINE RELEASE FROM THE GUINEA‐PIG TAENIA CAECUM EVOKED BY ELECTRICAL FIELD STIMULATION, NICOTINE AND PERIVASCULAR NERVE STIMULATION

Potentiation by Dilazep of the Negative Inotropic Effect of Adenosine on Guinea‐pig Atria

Comparative studies of purinergic nerves

Contact Info

Product

Resources

About

[³H]‐adenine nucleotide and [³H]‐noradrenaline release evoked by electrical field stimulation, perivascular nerve stimulation and nicotine from the taenia of the guinea‐pig caecum

EFFECT OF 6‐HYDROXYDOPAMINE ON [³H]‐ADENINE NUCLEOTIDE AND [³H]‐NORADRENALINE RELEASE FROM THE GUINEA‐PIG TAENIA CAECUM EVOKED BY ELECTRICAL FIELD STIMULATION, NICOTINE AND PERIVASCULAR NERVE STIMULATION

EFFECT OF 6‐HYDROXYDOPAMINE ON [³H]‐ADENINE NUCLEOTIDE AND [³H]‐NORADRENALINE RELEASE FROM THE GUINEA‐PIG TAENIA CAECUM EVOKED BY ELECTRICAL FIELD STIMULATION, NICOTINE AND PERIVASCULAR NERVE STIMULATION