³¹P chemical shift imaging of the regionally ischemic perfused heart

Abstract

The application of one-dimensional 31P chemical shift imaging to the study of the isolated perfused rat heart is described. Its applicability for the examination of regional ischemia in the isolated perfused rat heart is demonstrated. The data sets show clearly the localization to the ischemic and nonischemic regions of the heart. Such studies will permit the correlation of cardiac metabolism and function in a controlled manner that is not possible in the application of these techniques to the intact animal. In particular, the time resolution of the experiments will enable spatially localized metabolic studies to be performed during the onset of ischemia and during reperfusion.

High‐resolution 1H NMR imaging of regional ischemia in the isolated perfused rabbit heart at 4.7 T

“…imaging. Experiments were performed on a General Electric CSI 4.7-T/40cm bore system interfaced with a homebuilt gradient and radiofrequency assembly as previously described ( 19,20). Gradients of up to 5 G/cm could be generated with a switching time of approximately 100 ps, without additional preemphasis.…”

Common-Mode Differential-Mode (CMDM) Method for Double-Nuclear MR Signal Excitation and Reception at Ultrahigh Fields

“…Heteronuclear MRS/MRSI has been widely used for noninvasive studies of bioenergetics [6], [8], [9], metabolism [1]–[3], [7], [10], [11], [13], [16], stroke [25], myocardial infarction [4] and cellular physiology [12], [17], drug dynamics and delivery [5], [26], [27] and direct detection of cerebral metabolic rate of oxygen [28]–[30]. However, the low sensitivity of the heteronuclei (mainly due to their low natural abundance) significantly limits performance, especially for in vivo applications.…”

Phased spectroscopic images: Application to the characterization of the 1H 1.3‐ppm resonance in intracerbral tumors in the rat

“…To recover the sign of the resonances, the magnitude mode, which does not broaden the lines if the complete echo is acquired (13), cannot be used and the spectra must be phased. Phased spectroscopic images were generally obtained by phasing each spectrum of the image, individually (14)(15)(16)(17). However, manual phasing can be impractical when large, multidimensional images are acquired.…”