<sup>31</sup>P NMR <i>in vivo</i> study of the acute effects of different doses of γ‐radiation on rat brain phosphate metabolism

Semenova, N. A.; Yushmanov, Victor E.; Konradov, A. A.

doi:10.1002/nbm.1940070502

Cited by 5 publications

(4 citation statements)

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“…Signal 5 decreased in 3 rats and increased in 1 rat. Two rats demonstrated simultaneous increase in the level of monophosphate esters and P. 1 These shifts are significant (p<0.05) but weak (less than 35%). The common feature of all rats was stability of ATP, ADP, and pH levels.…”

Section: -J7mentioning

confidence: 84%

“…Regulation of energy metabolism modifies interrelation between phosphates involved in this process, as evidenced by in vivo 3~p-NMR data [1,7]. Analysis of individual correlation matrices revealed lack of repeated significant correlations in the matrices for intact brain (p<0.05).…”

Section: -J7mentioning

confidence: 97%

“…Local disturbances in cerebral circulation result in individual disturbances in the phosphate metabolism [1,7], which disappear after statistical averaging. Therefore, this paper focuses on variability of individual indices of cerebral energy metabolism in the studied model and assesses general regularities characterizing cerebral energy metabolism during local disturbances in cerebral blood flow.…”

Section: Abstract: Nuclear Magnetic Resonance In Vivo; Brain; Local mentioning

confidence: 99%

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Individual peculiarities of cerebral energy metabolism during local ischemia.In vivo nuclear magnetic resonance spectroscopy data

Semenova¹,

Topchyan

Мирзоян

et al. 1999

Bull Exp Biol Med

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The cerebral content of lactate and phosphate metabolites and intracellular pH were studied in intact rats 3 and 8 days after ligation of the middle cerebral artery. Each rat exhibited accumulation of lactate and individual changes in creatinine phosphate, inorganic phosphorus, and monophosphate esters, while the level of ATP, ADP, and pH were constant. Regulatory changes in the system of cerebral energy metabolism are characterized by the correlation analysis of these parameters. Key Words: nuclear magnetic resonance in vivo; brain; local ischemia; energy metabol&mDisturbances in energy metabolism are a key factor underlying damage to the nervous tissue during cerebral isehemia.Our aim was to characterize the disturbances in cerebral energy metabolism in viva in a new model of local ischemia produced by proximal ligation of the middle cerebral artery.Local disturbances in cerebral circulation result in individual disturbances in the phosphate metabolism [1,7], which disappear after statistical averaging. Therefore, this paper focuses on variability of individual indices of cerebral energy metabolism in the studied model and assesses general regularities characterizing cerebral energy metabolism during local disturbances in cerebral blood flow. MATERIALS AND METHODSThe study was carried out on 7 mature male Wistar rats weighing 250-280 g. Local ischemia was pro-

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Section: -J7mentioning

confidence: 84%

Section: -J7mentioning

confidence: 97%

Section: Abstract: Nuclear Magnetic Resonance In Vivo; Brain; Local mentioning

confidence: 99%

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Individual peculiarities of cerebral energy metabolism during local ischemia.In vivo nuclear magnetic resonance spectroscopy data

Semenova¹,

Topchyan

Мирзоян

et al. 1999

Bull Exp Biol Med

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“…Brain spectra were recorded in an NMR-spectrometer AM-400WB (Bruker) at a frequency of 161.98 MHz for 31 P nuclei using a sensor with a surface coil, which was constructed for NMR studies of rat brain in vivo [6]. The conditions of spectrum recording were described in [7].…”

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confidence: 99%

Study of the Effect of Preconditioning with Succinic Acid Salt of Choline (1:2) on the Disturbances of Energy Metabolism in the Brain during Ischemia by 31P NMR In vivo

Помыткин¹,

Семенова

2005

Dokl Biochem Biophys

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It is known that short-term hypoxia or ischemia may temporarily increase brain resistance to subsequent ischemic insult [1,2]. The mechanisms of this phenomenon, which was termed preconditioning, are not studied well.One of the consequences of ischemia is a rapid simultaneous increase in the concentrations of several metabolites, including succinic acid (SA) and choline [3]. A role of these metabolites as potential mediators of preconditioning is not studied.The purpose of this work was to study the role of metabolites, which concentration is increased during ischemia, in the protective effect of preconditioning. The levels of phosphates, which are the main players in the energy metabolism of brain, were studied by NMR spectroscopy in vivo in rats preconditioned with the model drug succinic acid salt of choline (1:2) (SSC) using the model of total ischemia.The dynamics of phosphates involved in energy metabolism during total brain ischemia was used as an assessment criterion of the preconditioning effect. This selection was based on the fact that a decrease in the brain energy status due to deficiency in the blood-transported energy substrates and oxygen, required for ATP synthesis, is the triggering mechanism in the development of injuries induced by the brain ischemia [4].Because NMR spectroscopy in vivo , is a noninvasive method, it excludes experimental artifacts associated with the extraction of brain tissues and provides continuous monitoring of parameters detected, such as phosphates [5].In this study we demonstrated that SSC, administered into animals as a preconditioning agent, decelerates a decrease in the concentration of energy-rich phosphates (ATP and creatine phosphate (CP)) and a decrease in energy status of rat brain during total ischemia. Therefore, SSC may be regarded a mediator of ischemic preconditioning.SSC (10% solution in saline; dose, 10 mg/kg body weight) was injected into male Wistar rats (the experimental group) daily for seven days. The control animals were injected with saline.The levels of phosphate metabolites (such as ATP, CP, inorganic phosphate (P i ), and phosphate monoesters (PME)) and intracellular pH were measured in animals narcotized with Nembutal on day 8 after the beginning of the experiment using in vivo 31 P NMR.Brain spectra were recorded in an NMR-spectrometer AM-400WB (Bruker) at a frequency of 161.98 MHz for 31 P nuclei using a sensor with a surface coil, which was constructed for NMR studies of rat brain in vivo [6]. The conditions of spectrum recording were described in [7].Total ischemia in rats was modeled immediately after measurement in the sensor of the NMR spectrometer by arresting the heart with the use of alternating current at 27 V. The current was applied for 4 min on a pair of needle electrodes fixed under the skin of the chest. The 31 P brain spectra were recorded in vivo at 1-min intervals starting from the first minute. Each spectrum was recorded for 1 min. Measurements were performed for 15 min.The spectrum processing is described in [7]. The levels...

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³¹P NMR in vivo study of rat brain energy metabolism after frontal cortex injury. a method based on convoluted correlation matrices of the spectral data

1995

Self Cite

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Disturbances of energy metabolism in rat brain after frontal lobectomy and post-traumatic effects of monosialic ganglioside GM1 on brain energy metabolism were studied by 31P NMR in vivo. Frontal lobectomy caused the statistically significant reversible decrease of ATP and increase of ADP during the compensatory period. In a presence of GM1 these post-operational effects were not observed. The mean value of normalized correlation coefficients between NMR-measured phosphomonoesters, P(i), pH, phosphocreatine, gamma-ATP + beta-ADP, alpha-ATP + alpha-ADP +NAD/NADH + diphosphodiesters and beta-ATP for a given group of animals (Z-index) was used to estimate brain energetic status. It has been shown that the variation of the Z-index correlates with the dynamics of locomotor activity during the compensatory period. The Z-index increased by more than 100% for the subgroup with an increased moving activity and only by about 20% for the subgroup with a decreased activity. Moreover, the compositions of these subgroups were almost the same as those from the solution of the reverse task, i.e., the studied group was divided into two subgroups by the Z-index. The Z-index is proposed as a possible criterion of pathology detection in brain energetics.

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³¹P NMR in vivo study of the acute effects of different doses of γ‐radiation on rat brain phosphate metabolism

Cited by 5 publications

References 10 publications

Individual peculiarities of cerebral energy metabolism during local ischemia.In vivo nuclear magnetic resonance spectroscopy data

Individual peculiarities of cerebral energy metabolism during local ischemia.In vivo nuclear magnetic resonance spectroscopy data

Study of the Effect of Preconditioning with Succinic Acid Salt of Choline (1:2) on the Disturbances of Energy Metabolism in the Brain during Ischemia by 31P NMR In vivo

³¹P NMR in vivo study of rat brain energy metabolism after frontal cortex injury. a method based on convoluted correlation matrices of the spectral data

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31P NMR in vivo study of the acute effects of different doses of γ‐radiation on rat brain phosphate metabolism

Cited by 5 publications

References 10 publications

Individual peculiarities of cerebral energy metabolism during local ischemia.In vivo nuclear magnetic resonance spectroscopy data

Individual peculiarities of cerebral energy metabolism during local ischemia.In vivo nuclear magnetic resonance spectroscopy data

Study of the Effect of Preconditioning with Succinic Acid Salt of Choline (1:2) on the Disturbances of Energy Metabolism in the Brain during Ischemia by 31P NMR In vivo

31P NMR in vivo study of rat brain energy metabolism after frontal cortex injury. a method based on convoluted correlation matrices of the spectral data

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³¹P NMR in vivo study of the acute effects of different doses of γ‐radiation on rat brain phosphate metabolism

³¹P NMR in vivo study of rat brain energy metabolism after frontal cortex injury. a method based on convoluted correlation matrices of the spectral data