<sup>31</sup>P NMR studies of adenosine‐stimulated ATP synthesis in perfused luteinized ovaries

Haseltine, Florence P.; Arias‐Mendoza, Fernando; Kaye, Alvin M.; Degani, Hadassa

doi:10.1002/mrm.1910030515

Cited by 11 publications

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References 13 publications

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“…The real power of NMR may lie in its ability to make continuous non-destructive measurements of metabolic concentration. Haseltine et al 7 concentrated on perfused ovaries at the luteal phase and examined the effect of added adenosine on the concentration of ATP using 31 P NMR. However, changes in the phosphate metabolites and the physiologic role of ATP in the ovulatory process were not fully examined in these reports.…”

Section: Introductionmentioning

confidence: 99%

31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary

et al. 1999

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The concentrations of phosphate metabolites were measured in perfused rabbit ovaries before, during and after ischemia, and during the ovulatory process induced by the administration of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG) using 31 P NMR spectroscopy. A decrease in adenosine triphosphate (ATP) and an increase in inorganic phosphate (P i ) during ischemia were observed. After reperfusion of the ovary, both ATP and P i levels returned to their initial control levels. These results are in agreement with the results observed in other organ tissues. On the other hand, ATP levels rapidly decreased dose dependently with infusion of HCG during 10 h of perfusion. A decrease in ovarian ATP concentration after infusion of HCG was also confirmed by enzymatic analysis of the extracts from ovarian tissues. On the other hand, significant changes in P i levels were not observed throughout the perfusion period. Furthermore, significant changes in phosphomonoester (PME) levels, adenosine diphosphate (ADP) levels and intracellular pH were not observed before and during the infusion of HCG. The concentration of cyclic adenosine 3',5'-monophosphate (cAMP) in the perfusate after the perfusion was also measured during the ovulatory period. Within 30 min cAMP significantly increased dose dependently and reached peak levels within 60 min. The concentration of cAMP then gradually decreased. Ovulatory efficacy during the infusion of HCG was also observed. These results suggest that the decreased ATP in the successive processes leading to ovulation probably reflects protein phosphorylation through the activation of the cAMPdependent protein kinases prior to induction of various chemical reactions related to the initiation of ovulation.

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Section: Introductionmentioning

confidence: 99%

31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary

et al. 1999

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show abstract

31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary

et al. 1999

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The concentrations of phosphate metabolites were measured in perfused rabbit ovaries before, during and after ischemia, and during the ovulatory process induced by the administration of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG) using 31P NMR spectroscopy. A decrease in adenosine triphosphate (ATP) and an increase in inorganic phosphate (Pi) during ischemia were observed. After reperfusion of the ovary, both ATP and Pi levels returned to their initial control levels. These results are in agreement with the results observed in other organ tissues. On the other hand, ATP levels rapidly decreased dose dependently with infusion of HCG during 10 h of perfusion. A decrease in ovarian ATP concentration after infusion of HCG was also confirmed by enzymatic analysis of the extracts from ovarian tissues. On the other hand, significant changes in Pi levels were not observed throughout the perfusion period. Furthermore, significant changes in phosphomonoester (PME) levels, adenosine diphosphate (ADP) levels and intracellular pH were not observed before and during the infusion of HCG. The concentration of cyclic adenosine 3′,5′‐monophosphate (cAMP) in the perfusate after the perfusion was also measured during the ovulatory period. Within 30 min cAMP significantly increased dose dependently and reached peak levels within 60 min. The concentration of cAMP then gradually decreased. Ovulatory efficacy during the infusion of HCG was also observed. These results suggest that the decreased ATP in the successive processes leading to ovulation probably reflects protein phosphorylation through the activation of the cAMP‐dependent protein kinases prior to induction of various chemical reactions related to the initiation of ovulation. Copyright © 1999 John Wiley & Sons, Ltd.

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¹³C NMR kinetic studies of the rapid stimulation of glucose metabolism by estrogen in immature rat uterus

1994

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Glucose metabolism in control and estrogen stimulated rat uteri was investigated using 13C NMR spectroscopy. By employing an NMR adapted perifusion system, and developing protocols and methods based on the application of [1-13C]glucose labeling, it was possible to measure, with a temporal resolution of 10 min, the kinetics of glucose consumption, lactate production and 13C incorporation into glutamate, alanine and glycogen. In control immature rat uteri, under aerobic conditions, the rates (+/- SEM) of [1-13C]glucose consumption and [3-13C]lactate production were 24 +/- 2 and 7.5 +/- 0.5 mmol/g wet weight/h. The rates of synthesis of [4-13C]glutamate, [3-13C]alanine and 13C labeling of glycogen at C1 of its glucose moieties were significantly lower and were in the range 0.3-0.6 mmol/g wet weight/h. Thus, ca 35% of the labeled glucose was accounted for by the glycolytic and other observed pathways. In vitro stimulation of the uteri by estrogen was found to increase significantly, within 1 h, glucose consumption by 80%, lactate production by 150% and glutamate and glycogen synthesis by 150%, in parallel to a rapid hormonal induction of mRNA for the brain type isozyme of creatine kinase.

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³¹P NMR studies of adenosine‐stimulated ATP synthesis in perfused luteinized ovaries

Cited by 11 publications

References 13 publications

31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary

31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary

31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary

¹³C NMR kinetic studies of the rapid stimulation of glucose metabolism by estrogen in immature rat uterus

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31P NMR studies of adenosine‐stimulated ATP synthesis in perfused luteinized ovaries

Cited by 11 publications

References 13 publications

31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary

31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary

31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary

13C NMR kinetic studies of the rapid stimulation of glucose metabolism by estrogen in immature rat uterus

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³¹P NMR studies of adenosine‐stimulated ATP synthesis in perfused luteinized ovaries

¹³C NMR kinetic studies of the rapid stimulation of glucose metabolism by estrogen in immature rat uterus