<sup><b>13</b></sup>C Nuclear Magnetic Resonance Studies of Lipid Interactions in Single- and Multi-Component Lipid Vesicles

Stoffel, Wilhelm; Tunggal, Budi; Zierenberg, Ottfried; Schreiber, Ekkehard; Binczek, Erika

doi:10.1515/bchm2.1974.355.2.1367

Cited by 47 publications

(17 citation statements)

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“…As discussed earlier, the energy barrier separating a trans conformation from a gauche conformation is about 3.5 Kcal mol "l. The presence of a double bond, however, reduces the energy barrier for the adjacent C-C single bond rotations to about 2 Kcal mol 1 (20). Results obtained by IH-and 13C-NMR studies on phosphatidylcholine molecules with varying degrees of unsaturation are indeed compatible with the notion that an unsaturated acyl chain is intrinsically more mobile than a saturated one (24)(25)(26)(27).…”

Section: O O V Visupporting

confidence: 78%

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A structural model for the cholesterol‐phosphatidylcholine complexes in bilayer membranes

Huang

1977

Lipids

216

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Based on the structural properties of phospholipid and cholesterol molecules, and making use of the known structural and motional effects of cholesterol and its analogs on phospholipid bilayers, a model for the cholesterol-phosphatidylcholine complex is proposed. In this model, the 3beta-hydroxyl group of cholesterol is assumed to engage in hydrogen bonding with the carbonyl oxygen of the fatty acyl groups in phospholipids. Some specific configurations of the saturated and unsaturated fatty acyl chains of the phospholipid are suggested to participate in van der Waals attractive interactions with the apha and beta surface of the steroid nucleus.

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Section: O O V Visupporting

confidence: 78%

“…The proposed model predicts that the motion of the choline headgroup in bilayers should not be restricted by cholesterol addition. Evidence abundantly supports the prediction (11,17,26,(38)(39)(40)(41).…”

Section: The Structural Model For Cholesterol-phosphatidylcholine Commentioning

confidence: 52%

A structural model for the cholesterol‐phosphatidylcholine complexes in bilayer membranes

Huang

1977

Lipids

216

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“…An alternative explanation is that the blue shift of the early-gated spectrum, in the presence of cholesterol, is caused by a displacement of the dansyl groups, already in relatively nonpolar sites, to even less polar sites, presumably nearer the center of the bilayer. This we consider unlikely since the rigid sterol ring of cholesterol extends down to about Cl 1 of the lipid fatty acyl chains (Stoffel et al, 1974), and from molecular models, it seems unlikely that the dansyl group in dansylphosphatidylethanolamine could penetrate the bilayer as far as this.…”

Section: Resultsmentioning

confidence: 91%

Time-resolved emission spectroscopy of the dansyl fluorescence probe

Ghiggino¹,

Lee²,

Meech³

et al. 1981

Biochemistry

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Using a cavity-dumped laser system as the light source, we have recorded time-resolved fluorescence spectra for the dansyl group in a variety of systems. The fluorescence spectrum of dansylphosphatidylethanolamine incorporated into a variety of lipid bilayer systems at ambient temperatures shows very marked time dependence, with red shifts of up to 40 nm over a 40-ns period. Similarly, large red shifts are observed for dansylamide in 1-butanol at low temperatures, which can be tentatively attributed to the initial rapid formation of an intramolecular charge transfer state, followed by reorientation of the polar solvent molecules. The time-resolved behavior in the lipid bilayer system can, by comparison, also be at least partly explained by solvent reorientation, but the possibility of heterogeneity of the binding site must also be considered. The complex time dependence of the fluorescence of the dansyl group in these lipid bilayer systems considerably complicates the interpretation of fluorescence quenching and fluorescence energy transfer experiments.

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“…These lipid dispersions were used for 13C-NMR measurements and subsequently for reassembling studies with apoHDL, apoA-I, and apoA-II. This procedure will be described elsewhere (30). The lipid composition and the ratios of the individual lipids were analyzed quantitatively by thinlayer chromatography of aliquots in suitable solvent systems for phospholipids (31) and for cholesterol and cholesteryl esters (32).…”

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confidence: 99%

¹³ C Nuclear Magnetic Resonance Spectroscopic Evidence for Hydrophobic Lipid-Protein Interactions in Human High Density Lipoproteins

Stoffel

Zierenberg

Tunggal

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

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Phosphatidylcholines, sphingomyelins, cholesterol, and cholesterol esters were enriched with 3IC by chemical synthesis in specific positions of their hydrophilic groups and aliphatic chains. Their spin-lattice relaxation times were determined in organic solvents. The substances were organized as liposomes and recombined with total human high density apolipoproteins and the two separated main components, apolipoprotein A-I (apoLp-Gln-I) and apolipoprotein A-II (apoLp-Gln-II). The enrichment of '3C in the hydrophilic and hydrophobic moiety of these lipids increases the sensitivity of the method and allows the determination of T, times of special regions of these molecules in reasonable periods of time. It should give insight into hydrophilic and hydrophobic interactions of lipidprotein complexes.A readily accessible system for the study of lipid-lipid and lipid-protein interactions in their natural molecular organization is the high density lipoprotein (HDL) fraction of human serum. Detailed information is available on the lipid and protein composition of these particles. Two apoproteins, apoA-I and apoA-II, comprise almost 90% of the molecule, with a high degree of a-helical structure (61-70%) (3)(4)(5)(6)(7)(8)(9). The aminoacid sequence of the latter has been elucidated (10). The apoproteins are assembled with an approximately equal amount of lipids. The lipid components are mainly phosphatidylcholine, sphingomyelin, cholesterol esters, and cholesterol. The acyl residues of the ester lipids are predominantly unsaturated fatty acids (oleic and linoleic acid).The molecular organization of the molecules in the HDL particle relates closely to other important questions concerning the structure and interactions of lipids and proteins in more complex lipoprotein aggregates, such as biological membranes.Several models of the HDL structure have been proposed (7,(11)(12)(13)(14)(15)(16)(17)(18)(19)(20). There is no general agreement on any one of these structures, most likely because of insufficient knowledge of the lipid-protein interactions and the tertiary structure of the proteins.

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¹³C Nuclear Magnetic Resonance Studies of Lipid Interactions in Single- and Multi-Component Lipid Vesicles

Cited by 47 publications

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A structural model for the cholesterol‐phosphatidylcholine complexes in bilayer membranes

A structural model for the cholesterol‐phosphatidylcholine complexes in bilayer membranes

Time-resolved emission spectroscopy of the dansyl fluorescence probe

¹³ C Nuclear Magnetic Resonance Spectroscopic Evidence for Hydrophobic Lipid-Protein Interactions in Human High Density Lipoproteins

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13C Nuclear Magnetic Resonance Studies of Lipid Interactions in Single- and Multi-Component Lipid Vesicles

Cited by 47 publications

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A structural model for the cholesterol‐phosphatidylcholine complexes in bilayer membranes

A structural model for the cholesterol‐phosphatidylcholine complexes in bilayer membranes

Time-resolved emission spectroscopy of the dansyl fluorescence probe

13 C Nuclear Magnetic Resonance Spectroscopic Evidence for Hydrophobic Lipid-Protein Interactions in Human High Density Lipoproteins

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¹³C Nuclear Magnetic Resonance Studies of Lipid Interactions in Single- and Multi-Component Lipid Vesicles

¹³ C Nuclear Magnetic Resonance Spectroscopic Evidence for Hydrophobic Lipid-Protein Interactions in Human High Density Lipoproteins