2012
DOI: 10.1016/j.jsb.2011.12.015
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Super-resolution fluorescence imaging of chromosomal DNA

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Cited by 108 publications
(106 citation statements)
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“…Zessin and colleagues are the¯rst to successfully combine 5-Ethynyl-20-deoxyuridine (EdU) labeling and dSTORM technology for super-resolution imaging of DNA. 60 Although these emerging probes can improve the imaging quality of SMLM in some aspects, the category of biomolecules labeled by these strategies is limited and impedes their wide applications in super-resolution imaging.…”
Section: Emerging Small Molecular Labeling Methodsmentioning
confidence: 99%
“…Zessin and colleagues are the¯rst to successfully combine 5-Ethynyl-20-deoxyuridine (EdU) labeling and dSTORM technology for super-resolution imaging of DNA. 60 Although these emerging probes can improve the imaging quality of SMLM in some aspects, the category of biomolecules labeled by these strategies is limited and impedes their wide applications in super-resolution imaging.…”
Section: Emerging Small Molecular Labeling Methodsmentioning
confidence: 99%
“…This so-called bio-orthogonal 'click chemistry' has led to an explosion of interest in the selective covalent labeling of molecules in cells and living organisms (Kolb et al, 2001;Prescher and Bertozzi, 2005;Zessin et al, 2012). Click chemistry relies on a copper-catalyzed azide-alkyne cycloaddition (Box 1).…”
Section: Box 2 Reference Structures For Super-resolution Imagingmentioning
confidence: 99%
“…As a logical consequence, photoactivated localization microscopy (PALM) (Betzig et al, 2006;Shroff et al, 2007;Shroff et al, 2008), fluorescence photoactivation localization microscopy (FPALM) Hess et al, 2007) and stochastic optical reconstruction microscopy (STORM) using special pairs of fluorophores (Rust et al, 2006;Bates et al, 2007) were introduced shortly afterwards. A short time later, the use of commercially available organic fluorescent probes, such as fluorophore-labeled antibodies or phalloidin probes, was demonstrated for super-resolution imaging by direct STORM (dSTORM), facilitating the acceptance and broad applicability of localization microscopy Heilemann et al, 2009;van de Linde et al, 2011a;Jones et al, 2011;Dempsey et al, 2011;Sillibourne et al, 2011;Williamson et al, 2011;Kaminski-Schierle et al, 2011;van de Linde et al, 2012;Zessin et al, 2012;Lampe et al, 2012;Wilmes et al, 2012;Rossy et al, 2013;Mattila et al, 2013). Furthermore, other localization microscopy methods using photoswitching of standard organic fluorophores under slightly different experimental conditions emerged under the names of GSDIM (ground-state depletion microscopy followed by individual molecule return) (Fölling et al, 2008) and blink microscopy .…”
Section: Introductionmentioning
confidence: 99%
“…Stochastic LM imaging on isolated DNA has been demonstrated using DNA intercalating dyes [180,181], but this methodology still needs to be refined in order to be used in whole cells [182]. Recently, the Heilemann lab has reported on a click chemistry reaction for DNA labelling that allowed bright photoswitchable carbocyanine (Alexa 647) fluorophores to be introduced into DNA at a density compatible with dSTORM imaging at 20 nm resolution [183]. Chromosomal organisation in bacteria using super-resolution imaging has been studied with nucleoid-associated tagged proteins (HU, H-NS), revealing their distribution in clusters at different stages of the cell cycle [184] and hinting at a key role in global chromosomal organisation [185].…”
Section: Chromosome Organisationmentioning
confidence: 99%