Super-Resolution Fluorescence Microscopy System by Structured Light Illumination Based on Laser Interference

Gang, 文刚 Wen; Simin, 李思黾 Li; Xibin, 杨西斌 Yang; Linbo, 王林波 Wang; Yong, 梁永 Liang; Xin, 金鑫 Jin; Qian, 朱茜 Zhu; Hui, 李辉 Li

doi:10.3788/aos201737.0318003

光学学报

2017

DOI: 10.3788/aos201737.0318003

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Super-Resolution Fluorescence Microscopy System by Structured Light Illumination Based on Laser Interference

文刚 Wen Gang¹,

李思黾 Li Simin²,

杨西斌 Yang Xibin³

et al.

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HiFi-SIM: reconstructing high-fidelity structured illumination microscope images

Wen

Wang

et al. 2020

Preprint

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Structured illumination microscopy (SIM) has been a widely-used super-resolution (SR) fluorescence microscopy technique, but artifacts often appear in reconstructed SR images which reduce its fidelity and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly-seen artifacts without loss of fine structures and improve the axial sectioning. Since results of HiFi-SIM are not sensitive to used PSF and reconstruction parameters, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.

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HiFi-SIM: reconstructing high-fidelity structured illumination microscope images

Wen

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

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Super-Resolution Fluorescence Microscopy System by Structured Light Illumination Based on Laser Interference

Cited by 1 publication

References 0 publications

HiFi-SIM: reconstructing high-fidelity structured illumination microscope images

HiFi-SIM: reconstructing high-fidelity structured illumination microscope images

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