2024
DOI: 10.1002/sstr.202400231
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Super‐Resolution Imaging in Collagen‐Abundant Thick Tissues

Ya‐Han Chuang,
Yueh‐Feng Wu,
Ya‐Hui Lin
et al.

Abstract: Expansion microscopy (ExM) has gained increasing popularity for 3D ultrastructural imaging of cultured cells and tissue slices at nanoscale resolution using conventional microscopes via physical expansion of biological tissues. However, its application to collagen‐abundant thick tissues is still challenging. Herein, a new method, collagen ExM (ColExM), optimized for expanding tissues containing more than 70% collagen, is demonstrated. ColExM succeeds in 4.5‐fold linear expansion with minimal structural distort… Show more

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