Super-resolution infrared microspectroscopy reveals heterogeneous distribution of photosensitive lipids in human hair medulla

Sandt, Christophe; Borondics, Ferenc

doi:10.1016/j.talanta.2022.124152

Cited by 14 publications

(6 citation statements)

References 40 publications

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“…While IR spectroscopy itself has been considered to be “non-destructive” and can be used to image biological samples over time ranging from a few milliseconds to hours, O-PTIR uses two colinear infrared and visible lasers. Irradiation using one or both of these lasers could potentially damage and/or affect sample viability . While O-PTIR has been successfully used for microscopy-based spectroscopy of cells in vitro and Caenorhabditis elegans as experimental end points, it has not yet been reported if O-PTIR damages living tissue or organisms.…”

Section: Resultsmentioning

confidence: 99%

“…Irradiation using one or both of these lasers could potentially damage and/or affect sample viability. 24 While O-PTIR has been successfully used for microscopy-based spectroscopy of cells 17 in vitro and Caenorhabditis elegans as experimental end points, 7 it has not yet been reported if O-PTIR damages living tissue or organisms. Therefore, we examined metabolic activity after the collection of the O-PTIR spectra using a water-soluble tetrazolium-1 (WST1) assay and found that tissues retained metabolic activity after O-PTIR irradiation at levels similar to controls, indicating maintenance of tissue viability (Figure S3).…”

Section: ■ Resultsmentioning

confidence: 99%

“…Further details about the fundamentals of the technique and the instrument itself can be found in the refs and . To avoid the photodamage described for O-PTIR and use feasible settings for spectra collection, IR power was set to 22%, the IR pulse rate was set to 100 kHz, and Mirage DC was 1.005 V; green light probe power was 6% when the silicon photodiode detector (standard) had a gain of 10× or 0.6% for the avalanche photodiode detector (APD). These laser powers were selected because they provided the best signal-to-noise ratio and did not damage hydrated tissues.…”

Section: Methodsmentioning

confidence: 99%

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Label-Free High-Resolution Photothermal Optical Infrared Spectroscopy for Spatiotemporal Chemical Analysis in Fresh, Hydrated Living Tissues and Embryos

Gvazava,

Konings,

Cepeda-Prado

et al. 2023

J. Am. Chem. Soc.

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Label-free chemical imaging of living and functioning systems is the holy grail of biochemical research. However, existing techniques often require extensive sample preparation to remove interfering molecules such as water, rendering many molecular imaging techniques unsuitable for in situ structural studies. Here, we examined freshly extracted tissue biopsies and living small vertebrates at submicrometer resolution using optical photothermal infrared (O-PTIR) microspectroscopy and demonstrated the following major advances: (1) O-PTIR can be used for submicrometer structural analysis of unprocessed, fully hydrated tissue biopsies extracted from diverse organs, including living brain and lung tissues. ( 2) O-PTIR imaging can be performed on living organisms, such as salamander embryos, without compromising their further development. (3) Using O-PTIR, we tracked the structural changes of amyloids in functioning brain tissues over time, observing the appearance of newly formed amyloids for the first time. (4) Amyloid structures appeared altered following standard fixation and dehydration procedures. Thus, we demonstrate that O-PTIR enables time-resolved submicrometer in situ investigation of chemical and structural changes in diverse biomolecules in their native conditions, representing a technological breakthrough for in situ molecular imaging of biological samples.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Label-Free High-Resolution Photothermal Optical Infrared Spectroscopy for Spatiotemporal Chemical Analysis in Fresh, Hydrated Living Tissues and Embryos

Gvazava,

Konings,

Cepeda-Prado

et al. 2023

J. Am. Chem. Soc.

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“…The collection parameters were: spectral range 1790–1000 cm -1 , reflection mode at 2 cm -1 spectral resolution. To avoid photodamage, that has been described for O-PTIR 46 , IR power was 22 %, green light probe power was 6 %, pulse rate was set to 100 kHz, Mirage DC was 1.005 V, and 10x standard detector gain. Background spectra were collected on an in-built reference sample.…”

Section: Methodsmentioning

confidence: 99%

Label-free high-resolution infrared spectroscopy for spatiotemporal analysis of complex living systems

Gvazava

Konings

Cepeda-Prado

et al. 2023

Preprint

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Label-free chemical and structural imaging of complex living tissue and biological systems is the holy grail of biomedical research and clinical diagnostics. The current analysis techniques are time-consuming and/or require extensive sample preparation, often due to the presence of interfering molecules such as water, making them unsuitable for the analysis of such systems. Here, we demonstrate a proof-of-principle study using label-free optical photothermal mid-infrared microspectroscopy (O-PTIR) for fast, direct spatiotemporal chemical analysis of complex living biological systems at submicron resolution. While other analytical methods can provide only static snapshots of molecular structures, our O-PTIR approach enables time-resolved and in situ investigation of chemical and structural changes of diverse biomolecules in their native conditions. This comprises a technological breakthrough in infrared spectroscopy to analyze biomolecules under native conditions over time: in fresh unprocessed biopsies, living brain tissue, and vertebrates without compromising their viability.One-Sentence SummaryProof-of-principle application of non-destructive O-PTIR for high-resolution spatiotemporal chemical and structural analysis of unprocessed biopsies, living brain tissue, and vertebrates.

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“…Furthermore, a strong-intensity peak at 1096 cm -1 is attributed to the stretching vibrations of the C-O group within the carbon nanotube structure. It's worth noting that the region below 1000 cm -1 in the IR spectrum is commonly referred to as the ngerprint region, where peaks are predominantly observed with weak intensity [37].…”

Section: Ftir Analysismentioning

confidence: 99%

Reduction of Sulfur in Fuel oil Using Fe2O3 Hybrid Nanoadsorbent by Solvent Deasphalting and Optimization of Operational Parameters with CCD

Malek,

Samipourgiri,

Rashidi

et al. 2023

Preprint

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The present study investigated and tested the effect of adding three types of nanoadsorbents (multi-walled carbon nanotubes (MWCNT)) in pure form, multi-walled carbon nanotubes with Fe2O3 particles (MWCNT-Fe2O3) hybrid, and Silanated-Fe2O3 hybrid to heavy fuel oil to reduce sulfur using a deasphalting process with solvent. First, all three types of nanoadsorbents were synthesized. Then, the Central Composite Design (CCD) method was used to identify the parameters effective in deasphalting, such as the type of nanoadsorbent, the weight percentage of nanoadsorbent, and the solvent-to-fuel ratio, and to obtain their optimal values. Based on the optimization result, under laboratory temperature and pressure conditions, the highest percentage of sulfur reduction in deasphalted fuel (DAO) was obtained by adding 2.5% by weight of silanated-Fe2O3 nano-adsorbent and with a solvent-to-fuel ratio of 7.7 (The weight percentage of sulfur in DAO increased from 3.5% by weight to 2.46%, indicating a decrease of 30%). Additionally, by increasing the temperature to 70°C, in optimal conditions, the results revealed that the remaining sulfur percentage in DAO decreased to 2.13% by weight, indicating a decrease of 40%. Synthesized nanoadsorbents and asphaltene particles adsorbed on the surfaces of nanoadsorbents were evaluated by XRD, FTIR, FESEM, and TEM techniques.

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Super-resolution infrared microspectroscopy reveals heterogeneous distribution of photosensitive lipids in human hair medulla

Cited by 14 publications

References 40 publications

Label-Free High-Resolution Photothermal Optical Infrared Spectroscopy for Spatiotemporal Chemical Analysis in Fresh, Hydrated Living Tissues and Embryos

Label-Free High-Resolution Photothermal Optical Infrared Spectroscopy for Spatiotemporal Chemical Analysis in Fresh, Hydrated Living Tissues and Embryos

Label-free high-resolution infrared spectroscopy for spatiotemporal analysis of complex living systems

Reduction of Sulfur in Fuel oil Using Fe2O3 Hybrid Nanoadsorbent by Solvent Deasphalting and Optimization of Operational Parameters with CCD

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