Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

Abstract

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of...

Small-molecule photoswitches for fluorescence bioimaging: engineering and applications

“…2d). 40 In all FRET-based systems, the stability and fatigue resistance of the switch remain major limitations, as the decomposition of the switch progressively increases fluorescence background and lowers achievable contrast. Therefore, it is critical to pursue alternative strategies for robust photoswitchable fluorophores.…”

“…With synthetic fluorophores, several derivatives of rhodamines-spiropyran dyads showed fluorescence switching inside living cells. [37][38][39][40] Recently, the Anderson group systematically investigated cyanine and rhodamine fluorophores in FRET pair with spiropyrans derivatives. Early examples showed far-red fluorescence photoswitching in live cells, albeit with low fatigue resistance.…”

Cover Feature: Smart Photochromic Materials Triggered with Visible Light (Eur. J. Org. Chem. 19/2022)

“…Superresolution microscopy is an excellent tool for imaging both materials [186] and biological systems. [187] Structural optimization of photoswitchable fluorophores [188] towards compatibil-ity with aqueous environment [189] and activation with visible light (e. g. in spirooxazines [190] or diarylethenes [191] ) could expand the scope of this technology in complex biological setups. [192] Optogenetics is a technique to control activity of neurons or other cell types with light, by expression of light-sensitive ion channels, pumps, or enzymes.…”

Nanoparticles for super-resolution microscopy: intracellular delivery and molecular targeting

“…61,62 As they also possess larger two-photon absorption cross-sections (10 3 -10 4 GM) than typical fluorescent molecular dyes or fluorescent proteins, they have become an attractive choice for applications in SRM. 51,[63][64][65] Importantly, surfaces of QD nanocrystals can be post-functionalized with a variety of biomolecules (e.g., antibodies, peptides, proteins, DNA, and vitamins) through well-known conjugation techniques (Fig. 6).…”