Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection

Zhi, Ya'nan; Wang, Benquan; Yao, Xincheng

doi:10.1615/critrevbiomedeng.2016016445

Cited by 7 publications

(4 citation statements)

References 119 publications

(106 reference statements)

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“…However, it is important to clarify that the movement magnitude of the photoreceptors in the isolated retina may differ from that in the natural condition in which the ROSs interface with the RPE. For pursuing in vivo investigation, we are constructing a super-resolution ophthalmoscope, based on virtually structured detection (VSD) [30][31][32][33].…”

Section: Discussionmentioning

confidence: 99%

Stimulus-evoked outer segment changes occur before the hyperpolarization of retinal photoreceptors

Wang

Pepperberg

et al. 2016

Biomed. Opt. Express

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Transient retinal phototropism (TRP) has been predominantly observed in rod photoreceptors activated by oblique visible light stimulation. Dynamic confocal microscopy and optical coherence tomography (OCT) have revealed rod outer segment (ROS) movement as the physical source of TRP. However, the physiological source of ROS movement is still not well understood. In this study, concurrent near-infrared imaging of TRP and electroretinogram (ERG) measurement of retinal electrophysiology revealed that ROS movement occurs before the onset of the ERG a-wave, which is known to reflect the hyperpolarization of retinal photoreceptors. Moreover, substitution of normal superfusing medium with low-sodium medium reversibly blocked the photoreceptor ERG a-wave, but largely preserved the stimulus-evoked ROS movements. Our experimental results and theoretical analysis indicate that early, disc-based stages of the phototransduction cascade, which occur before the hyperpolarization of retinal photoreceptors, contribute to the TRP associated ROS movement. 259-267 (1975). 29. E. Hessel, A. Herrmann, P. Müller, P. P. Schnetkamp, and K. P. Hofmann, "The transbilayer distribution of phospholipids in disc membranes is a dynamic equilibrium evidence for rapid flip and flop movement," Eur. J.

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Section: Discussionmentioning

confidence: 99%

Stimulus-evoked outer segment changes occur before the hyperpolarization of retinal photoreceptors

Wang

Pepperberg

et al. 2016

Biomed. Opt. Express

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“…Basic principle of VSD processing has been described previously. 14,25,30 Here we focus on the process of retrieving the MTF of the imaging system. As the modulation is achieved by using digital mask, two modulation phases (α = 0 and π/2) can be employed to solve equation (8) …”

Section: In Vivo Super-resolution Ophthalmoscopy Of Human Retinasmentioning

confidence: 99%

Super-resolution ophthalmoscopy: Virtually structured detection for resolution improvement in retinal imaging

Yao

Wang

et al. 2020

Exp Biol Med (Maywood)

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Quantitative retinal imaging is essential for advanced study and clinical management of eye diseases. However, spatial resolution of retinal imaging has been limited due to available numerical aperture and optical aberration of the ocular optics. Structured illumination microscopy has been established to break the diffraction-limit resolution in conventional light microscopy. However, practical implementation of structured illumination microscopy for in vivo ophthalmoscopy of the retina is challenging due to inevitable eye movements that can produce phase artifacts. Recently, we have demonstrated the feasibility of using virtually structured detection as one alternative to structured illumination microscopy for super-resolution imaging. By providing the flexibility of digital compensation of eye movements, the virtually structured detection provides a feasible, phase-artifact-free strategy to achieve super-resolution ophthalmoscopy. In this article, we summarize the technical rationale of virtually structured detection, and its implementations for super-resolution imaging of freshly isolated retinas, intact animals, and awake human subjects.

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“…Light microscopy is an indispensable tool for examining the subcellular details of biological structures, but its spatial resolution is limited to approximately half the wavelength of the illumination light. In their paper, 4 "Super-resolution scanning laser microscopy based on virtually structured detection," Zhi and coauthors describe a new technique that can be safely used in clinical ophthalmoscopy to examine retinal structures.…”

Section: A U T H O R P R O O Fmentioning

confidence: 99%

Special Issue on Research in Biomedical Engineering at the University of Illinois at Chicago: Fifty Years of Excellence

Magin

Royston

2015

Crit Rev Biomed Eng

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Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection

Cited by 7 publications

References 119 publications

Stimulus-evoked outer segment changes occur before the hyperpolarization of retinal photoreceptors

Stimulus-evoked outer segment changes occur before the hyperpolarization of retinal photoreceptors

Super-resolution ophthalmoscopy: Virtually structured detection for resolution improvement in retinal imaging

Special Issue on Research in Biomedical Engineering at the University of Illinois at Chicago: Fifty Years of Excellence

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