Supercontinuum generation for coherent anti-Stokes Raman scattering microscopy with photonic crystal fibers

Klarskov, Pernille; Isomäki, Antti; Hansen, Kristoffer Lindskov; Andersen, Peter E.

doi:10.1364/oe.19.026672

Cited by 23 publications

(6 citation statements)

References 30 publications

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“…These synchronous beams are difficult to produce without using two synchronized lasers or complex setups involving OPA and OPOs, or four-wave-mixingbased sources which are the current state-of-the-art methodologies. However, these beams are also readily produced through SC generation [87] using fs lasers pumping PCFs tailored to produce appropriate wavelengths. Temporally coherent SC radiation has so far only provided low peak powers and low spectral power density.…”

Section: F Coherent Anti-stokes Raman Scattering (Cars) Microscopymentioning

confidence: 99%

Supercontinuum radiation in fluorescence microscopy and biomedical imaging applications

Poudel

Kaminski

2019

J. Opt. Soc. Am. B

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Compact, high brightness supercontinuum sources have already made a big impact in the fields of fluorescence microscopy and other biomedical imaging techniques, such as OCT and CARS. In this review, we provide a brief overview on the generation and properties of supercontinuum radiation for imaging applications. We review specific uses of supercontinuum sources and their potential for imaging, but also their limitations and caveats. We conclude with a review of recent advances in UV supercontinuum generation, near-IR microscopy, exciting new potentials for the use of hollow core PCFs, on-chip supercontinuum generation and technologies to improve supercontinuum stability for certain applications.

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Section: F Coherent Anti-stokes Raman Scattering (Cars) Microscopymentioning

confidence: 99%

Supercontinuum radiation in fluorescence microscopy and biomedical imaging applications

Poudel

Kaminski

2019

J. Opt. Soc. Am. B

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“…The output beam of the compact diode-pumped Ti:sapphire femtosecond oscillator is split by a 60:40 beam splitter into the pump (200 mW) and the Stokes arm (300 mW). The light for the Stokes beam is then obtained by means of a polarization-maintaining photonic crystal fiber (PCF 5 from Klarskov et al 30 ) which generates a broad continuum ranging from visible to nearinfrared spectral wavelengths. 30 An 18 nm FWHM portion of a smooth broad peak generated around 1050 nm is filtered and subsequently amplified in a polarization maintaining singlemode ytterbium-doped fiber amplifier, to reach the desired pulse energy in the Stokes beam before recombining it with the pump beam.…”

Section: Epi-detecting Multimodal Hyperspectral Microscopementioning

confidence: 99%

Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser

Andreana

Le²,

Hansen

et al. 2017

J. Biomed. Opt

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-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser," J. Biomed. Opt. 22(9), 091517 (2017), doi: 10.1117/1.JBO.22.9.091517. Abstract. We have developed an epi-detected multimodal nonlinear optical microscopy platform based on a compact and cost-effective laser source featuring simultaneous acquisition of signals arising from hyperspectral coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence, and second harmonic generation. The laser source is based on an approach using a frequency-doubled distributed Bragg reflector-tapered diode laser to pump a femtosecond Ti:sapphire laser. The operational parameters of the laser source are set to the optimum trade-off between the spectral and temporal requirements for these three modalities, achieving sufficient spectral resolution for CARS in the lipid region. The experimental results on a biological tissue reveal that the combination of the epi-detection scheme and the use of a compact diode-pumped femtosecond solid-state laser in the nonlinear optical microscope is promising for biomedical applications in a clinical environment. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…Multiplex-CARS (M-CARS) experiments, based on simultaneous excitation of all frequencies ranging from 0 to 4,500 cm −1 (using a monochromatic pump beam and a large band Stokes wave), have been demonstrated in 2002, by mixing polychromatic and monochromatic laser sources (Müller and Schins, 2002). In this framework, supercontinuum sources appeared as the best way to implement M-CARS systems (Okuno et al, 2007;Klarskov et al, 2011;Shen et al, 2018). However, because of the group velocity dispersion encountered in optical fibers, laser sources with long pulses have been privileged to guaranty temporal overlap between all the frequencies constituting the continuum and the quasimonochromatic pump signals (Okuno et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Optimizing supercontinuum spectro-temporal properties by leveraging machine learning towards multi-photon microscopy

et al. 2022

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Multi-photon microscopy has played a significant role in biological imaging since it allows to observe living tissues with improved penetration depth and excellent sectioning effect. Multi-photon microscopy relies on multi-photon absorption, enabling the use of different imaging modalities that strongly depends on the properties of the sample structure, the selected fluorophore and the excitation laser. However, versatile and tunable laser excitation for multi-photon absorption is still a challenge, limited by e.g. the narrow bandwidth of typical laser gain medium or by the tunability of wavelength conversion offered by optical parametric oscillators or amplifiers. As an alternative, supercontinuum generation can provide broadband excitations spanning from the ultra-violet to far infrared domains and integrating numerous fluorophore absorption peaks, in turn enabling different imaging modalities or potential multiplexed spectroscopy. Here, we report on the use of machine learning to optimize the spectro-temporal properties of supercontinuum generation in order to selectively enhance multi-photon excitation signals compatible with a variety of fluorophores (or modalities) for multi-photon microscopy. Specifically, we numerically explore how the use of reconfigurable (femtosecond) pulse patterns can be readily exploited to control the nonlinear propagation dynamics and associated spectral broadening occurring in a highly-nonlinear fiber. In this framework, we show that the use of multiple pulses to seed optical fiber propagation can trigger a variety of nonlinear interactions and complex propagation scenarios. This approach, exploiting the temporal dimension as an extended degree of freedom, is used to maximize typical multi-photon excitations at selected wavelengths, here obtained in a versatile and reconfigurable manner suitable for imaging applications. We expect these results to pave the way towards on-demand and real time supercontinuum shaping, with further multi-photon microscopy improvements in terms of spatial 3D resolution, optical toxicity, and wavelength selectivity.

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Supercontinuum generation for coherent anti-Stokes Raman scattering microscopy with photonic crystal fibers

Cited by 23 publications

References 30 publications

Supercontinuum radiation in fluorescence microscopy and biomedical imaging applications

Supercontinuum radiation in fluorescence microscopy and biomedical imaging applications

Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser

Optimizing supercontinuum spectro-temporal properties by leveraging machine learning towards multi-photon microscopy

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