Supercritical carbon dioxide decellularised pericardium: Mechanical and structural characterisation for applications in cardio-thoracic surgery

Halfwerk, F.R.; Rouwkema, Jeroen; Gossen, Jan A.; Grandjean, Jan G.

doi:10.1016/j.jmbbm.2017.10.002

Cited by 27 publications

(26 citation statements)

References 47 publications

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“…Our results also show that the decellularization process lowers the GAGs content in the tissue. Even though in [ 28 ] this protocol enabled the decellularization of porcine and bovine pericardium without significant effect on its mechanical and structural properties, it appeared unsuitable for the decellularization of ovine aortic roots. The caustic nature of the alkali caused the loss of elasticity of the valve leaflet ECM that may promote overt fibrosis, detachment, or even apoptosis [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the high pressure in the reactor can burst the cells inside the tissue followed by the removal of cellular fragments upon rapid depressurization. When employed in a combination with traditional decellularizing agents [ 28 , 29 , 30 ] or co-solvents such as ethanol [ 22 , 31 , 32 ], scCO 2 assists the decellularization and reduces the risk of contamination and processing time.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

et al. 2020

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One of the leading trends in the modern tissue engineering is the development of new effective methods of decellularization aimed at the removal of cellular components from a donor tissue, reducing its immunogenicity and the risk of rejection. Supercritical CO2 (scCO2)-assisted processing has been proposed to improve the outcome of decellularization, reduce contamination and time costs. The resulting products can serve as personalized tools for tissue-engineering therapy of various somatic pathologies. However, the decellularization of heterogeneous 3D structures, such as the aortic root, requires optimization of the parameters, including preconditioning medium composition, the type of co-solvent, values of pressure and temperature inside the scCO2 reactor, etc. In our work, using an ovine aortic root model, we performed a comparative analysis of the effectiveness of decellularization approaches based on various combinations of these parameters. The protocols were based on the combinations of treatments in alkaline, ethanol or detergent solutions with scCO2-assisted processing at different modes. Histological analysis demonstrated favorable effects of the preconditioning in a detergent solution. Following processing in scCO2 medium provided a high decellularization degree, reduced cytotoxicity, and increased ultimate tensile strength and Young’s modulus of the aortic valve leaflets, while the integrity of the extracellular matrix was preserved.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

et al. 2020

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“…However, structural derangements (porosity) in both the aortic wall and valve leaflet ECM and deprivation of spongiosa that was the hallmarks of this protocol, regardless of the pressure value (10 or 15 MPa). Even though in [27] this protocol enabled decellulariztion of porcine and bovine pericardim without significant effect on its mechanical and structural properties, it appeared unsuitable for the decellularization of ovine aortic root.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the minimal environmental impact, the scCO 2 extraction is referred to as a method of the so-called "green chemistry" and is widely used in the food, textile, perfume and cosmetic industries [25,26]. scCO 2 can act either as an independent solvent, or in combination with traditional decellularizing agents [27][28][29] or co-solvents such as ethanol [22,30,31]. The use of scCO 2 significantly improves the decellularization outcome, reduces the risk of contamination and processing time.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of supercritical CO2 extraction protocols in a model of ovine aortic root decellularization

Gafarova¹,

Grebenik²,

Lazhko³

et al. 2020

Preprint

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One of the leading trends in the modern tissue engineering is the development of new effective methods of decellularization aimed at the removal of cellular components from donor tissue reducing its immunogenicity and the risk of rejection. Supercritical CO2 (scCO2) extraction can significantly improve the outcome of decellularization, reduce contamination and time costs.The resulting products can serve as personalized tools for tissue-engineering therapy of various somatic pathologies. However, decellularization of complex 3D structures, such as the aortic root, requires optimization of the parameters, including preconditioning medium composition, type of co-solvent, values of pressure and temperature insight the scCO2 reactor, etc. In our work, using an ovine aortic root model, we performed a comparative analysis of the efficiency of decellularization using approaches based on various combinations of these parameters. The protocols were based on combinations of treatment in alkaline, ethanol or detergent solutions with scCO2 extraction at different modes. Based on a histological analysis, we have selected an optimal protocol for the decellularization of ovine aortic root employing preconditioning in a detergent solution. The positive effects of scCO2 on the decellularization extent, cytotoxicity and histoarchitecture of the tissue were demonstrated.

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“… 44 Supercritical CO 2 has been used in the decellularisation of rat heart tissues, porcine corneas, porcine and bovine pericardium, and human adipose tissue. 45 – 48 All the cited articles reported successful decellularisation with preservation of structural and mechanical properties, thus making supercritical CO 2 a promising method.…”

Section: Methods To Produce Decellularised Matricesmentioning

confidence: 99%

Decellularised scaffolds: just a framework? Current knowledge and future directions

et al. 2020

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The use of decellularised matrices as scaffolds offers the advantage of great similarity with the tissue to be replaced. Moreover, decellularised tissues and organs can be repopulated with the patient’s own cells to produce bespoke therapies. Great progress has been made in research and development of decellularised scaffolds, and more recently, these materials are being used in exciting new areas like hydrogels and bioinks. However, much effort is still needed towards preserving the original extracellular matrix composition, especially its minor components, assessing its functionality and scaling up for large tissues and organs. Emphasis should also be placed on developing new decellularisation methods and establishing minimal criteria for assessing the success of the decellularisation process. The aim of this review is to critically review the existing literature on decellularised scaffolds, especially on the preparation of these matrices, and point out areas for improvement, finishing with alternative uses of decellularised scaffolds other than tissue and organ reconstruction. Such uses include three-dimensional ex vivo platforms for idiopathic diseases and cancer modelling.

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Supercritical carbon dioxide decellularised pericardium: Mechanical and structural characterisation for applications in cardio-thoracic surgery

Cited by 27 publications

References 47 publications

Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

Evaluation of supercritical CO2 extraction protocols in a model of ovine aortic root decellularization

Decellularised scaffolds: just a framework? Current knowledge and future directions

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