Supercritical carbon dioxide extraction of rice bran oil and column partition fractionation of γ-oryzanols

Extraction and characteristics of seed kernel oil from white pitaya

“…It has proved to be effective, highyielding and energy saving (Pan et al, 2003;Shu et al, 2003). CO 2 in its supercritical state has been used as solvent in supercritical fluid extraction (SFE) to extract nonpolar and thermally sensitive components from natural plants (Chen et al, 2008;Grosso et al, 2008;Zaidul et al, 2006). SFE-CO 2 offers the possibility of developing a process for oil extraction and purification, which avoids the use of organic solvents and large waste streams (Zaidul et al, 2006(Zaidul et al, , 2007.…”

Hypolipidemic Effects of Starch and γ-Oryzanol fromwx/aeDouble-Mutant Rice on BALB/c.KOR-ApoeshlMice

“…A total of 1 kg of wx/ae brown rice flour was soaked twice in 3 volumes of n-hexane at room temperature for 16 h. The extract was then filtered and evaporated under reduced pressure to yield a crude rice oil. We obtained pure rice oil by dewaxing and degumming the crude rice oil according to the method of Zullaikah et al 25) A -oryzanol-rich fraction was separated from the pure rice oil according to the method of Chen et al 26) Briefly, rice oil dissolved in n-hexane was loaded into an open column (550 mm long and 30 mm i.d.) packed with silica gel 60 (Nacalai Tesque, Kyoto, Japan).…”

Supercritical CO₂ Extraction of Rice Bran Oil –the Technology, Manufacture, and Applications

“…Typically, the saponifiable lipids include triaclyglycerols 47 monounsaturated fats, 33 polyunsaturated fats, and 20 saturated fats , glycolipids and phospholipids. Unsaponifiable lipids in RBO are tocopherols, tocotrienols, γ-oryzanols, sterols and carotenoids 14 , most of which have been reported for nutritional benefits such as antioxidative and chemopreventive properties 13 . RBO is the richest source of natural oryzanols.…”

“…With combination of a normal-phase medium-pressure column partition fractionation, γ-oryzanols were successfully concentrated up to 37 wt of RBO 14 .…”

“…For applications, a number of researchers have employed RSM and CCD methodology on SC-CO 2 extraction of RBO. It has been used for supercritical extraction of RBO with high γ-oryzanol content 14,41 . Operation parameters were optimized for the highest oil recovery 47 , for oil with highest antioxidants 44 , and for the best operation processes of RBO deacidfication 39 .…”