Supercritical CO₂ extraction of accumulated capsidiol from biotic elicitor‐activated Capsicum annuum L fruit tissues

Abstract

This work investigates the supercritical CO 2 extraction of capsidiol from pepper fruit tissues activated with Alternaria alternate (Fr) Keissler suspension culture as a biotic elicitor. Capsidiol production in the fruit tissue was markedly increased by the treatment with a biotic elicitor and reached its maximum level after 4 days of elicitation. The effects of separation parameters such as temperature, pressure, supercritical solvent flow rate, particle diameter and also initial capsidiol concentration were investigated on solubility, initial extraction rate and extraction yield. The optimal extraction conditions were obtained at the temperature of 40• C, the pressure of 400 bar, the supercritical CO 2 flow rate of 2 cm 3 min −1 , and the average particle diameter of 116 µm. The results showed that the ratio of the supercritical CO 2 extraction yield to the organic solvent extraction yield was changed from 84 to 97 wt-% depending on the initial capsidiol concentration.

Determination of Capsidiol in Tobacco Cells Culture by HPLC

“…The relative standard errors were 6.5% and 6.0% for OSE and SPE, respectively (Table I). These results are comparable with previous published work (22).…”

“…In previous studies, capsidiol content was determinated by thin-layer chromatography or gas chromatography (GC) after extraction with dichloromethane or chloroform (14,20). Also, a solid-phase extraction (SPE) method was used with absorption to polyamide and C 18 columns (21), and supercritical CO 2 extraction (22) has been used. Moreover, an high-performance liquid chromatography (HPLC) method employs cyanopropyl-bonded phase column for analysis of sesquiterpene phytoalexins such as capsidiol has been developed (23).…”