Supercritical CO2 extraction and online fractionation of dry ginger for production of high-quality volatile oil and gingerols enriched oleoresin

Shukla, Abhishek; Naik, S. N.; Goud, Vaibhav V.; Das, C. K.

doi:10.1016/j.indcrop.2019.01.005

Cited by 63 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Essential oils (EOs) are volatile aromatic substances extracted from different parts of plants. Until today, the main conventional methods used for EO extraction and separation include steam distillation and supercritical CO 2 extraction [ 8 , 9 ]. Because of the appreciable antibacterial properties, different kinds of EOs have been widely used as excellent substitutes for chemical-based preservatives in recent years [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

et al. 2020

View full text Add to dashboard Cite

Though essential oils exhibit antibacterial activity against food pathogens, their underlying mechanism is understudied. We extracted ginger essential oil (GEO) using supercritical CO2 and steam distillation. A chemical composition comparison by GC-MS showed that the main components of the extracted GEOs were zingiberene and α-curcumene. Their antibacterial activity and associated mechanism against Staphylococcus aureus and Escherichia coli were investigated. The diameter of inhibition zone (DIZ) of GEO against S. aureus was 17.1 mm, with a minimum inhibition concentration (MIC) of 1.0 mg/mL, and minimum bactericide concentration (MBC) of 2.0 mg/mL. For E. coli, the DIZ was 12.3 mm with MIC and MBC values of 2.0 mg/mL and 4.0 mg/mL, respectively. The SDS-PAGE analysis revealed that some of the electrophoretic bacterial cell proteins bands disappeared with the increase in GEO concentration. Consequently, the nucleic acids content of bacterial suspension was raised significantly and the metabolic activity of bacteria was markedly decreased. GEO could thus inhibit the expression of some genes linked to bacterial energy metabolism, tricarboxylic acid cycle, cell membrane-related proteins, and DNA metabolism. Our findings speculate the bactericidal effects of GEO primarily through disruption of the bacterial cell membrane indicating its suitability in food perseveration.

show abstract

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Ginger is widely cultivated in China. It has important uses in daily diet, industrial product production, traditional Chinese medicine, and so on (Wang et al, 2015;Marx et al, 2017;Shukla et al, 2019). However, there is a lack of research on ginger in the world, mainly due to the limitation of genome and the weak foundation of molecular biology.…”

Section: Introductionmentioning

confidence: 99%

Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis

Liu

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

Quantitative real-time PCR (qRT-PCR) is widely used in the detection of gene expression level. However, there is no suitable ginger reference gene for qPCR analysis. Therefore, it is the primary task to select and validate the appropriate ginger reference gene to normalize the expression of target genes. In this study, 14 candidate reference genes were selected and analyzed in different tissues (leaf, and rhizome), different development stages, different varieties, and abiotic stress (ABA and salt stress). Expression stability was calculated using geNorm and NormFinder, Bestkeeper, and RefFinder. For abiotic stress and total conditions, 28S and COX were identified as the most stable genes. In addition, RPII was the most stable in the different development stages and different varieties. TEF2 and RPL2 were the least stably expressed in the tissue and all the conditions. In order to verify the feasibility of these genes as reference genes, we used the most stable and least stable reference genes to normalize the expression levels of ZoSPS genes under different conditions. This work can provide theoretical support for future research on ginger gene expression.

show abstract

“…Zingiberene was found in a small amount, of 1.25%. The literature highlights the influence of many factors on the quantity and quality of ginger essential oil: pedoclimatic conditions, fresh or dry use [1] and extraction methods [24,38]. Evans WC (2002) mentions the presence of camphene, phellandrene, curcumene, terpineol, borneol and citral in ginger essential oil [16], which are compounds that were also found in the sample analysed in the present study.…”

Section: Gc-ms Analysismentioning

confidence: 70%

Gc-MS Analysis and Bioactive Properties of Zingiberis Rhizoma Essential Oil

Bucur¹

2020

FARMACIA

View full text Add to dashboard Cite

Ginger (Zingiber officinalis Roscoe) has been, from the past until now, one of the most worldwide cultivated aromatic plants. The fresh or dried underground ginger rhizome is used in medicinal purposes, which the European Pharmacopoeia presents as Zingiberis rhizoma. The essential oil obtained from ginger, available on the Romanian market, was prepared using the official method with the Neo-Clevenger apparatus. The amount of 1.925% (v/m) ± 0.049 was extracted, which is in accordance with the reference standards of the European Pharmacopoeia. The GC-MS analysis of essential oil composition revealed the presence of camphene (32.79%), eucalyptol (24.32%), α-pinene (18.05 %) and zingiberene (1.25%). Ginger essential oil has antioxidant properties with an IC50 value of 30.72 μL/mL in different acetone dilutions. The antifungal properties highlighted on isolated stems from public spaces (Fonsecaea sp. and Penicillium chrysogenum) may justify the use of ginger essential oil as an agent that prevents the exposure of the population to pathogens. RezumatGhimbirul (Zingiber officinalis Roscoe) a fost şi este una dintre cele mai cultivate plante aromate din lume. În scop medicinal, de la ghimbir se folosește rizomul, proaspăt sau uscat, pe care Farmacopeea Europeană îl prezintă în monografia Zingiberis rhizoma. Uleiul volatil obținut din ghimbirul comercializat pe piața din România, prin metoda oficinală, cu aparatul Neo-Clevenger, este în cantitate de 1,925% (v/m) ± 0,049, ceea ce corespunde prevederilor Farmacopeei Europene, care menționează un conținut de minim 1,5% (v/m). Analiza GC-MS a uleiului volatil a pus în evidență prezența camfenului (32,79%), eucaliptolului (24,32%), α-pinenului (18,05%) și zingiberenului (1,25%). Uleiul volatil de ghimbir prezintă proprietăți antioxidante cu valoarea IC 50 a diluțiilor, în acetonă, de 30,72 µL/mL. Proprietățile antifungice puse în evidență pe tulpini izolate din spațiile publice (Fonsecaea sp. și Penicillium chrysogenum) pot justifica utilizarea uleiului volatil de ghimbir ca agent de prevenire a expunerii populației la agenți patogeni.

show abstract

Supercritical CO2 extraction and online fractionation of dry ginger for production of high-quality volatile oil and gingerols enriched oleoresin

Cited by 63 publications

References 33 publications

Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis

Gc-MS Analysis and Bioactive Properties of Zingiberis Rhizoma Essential Oil

Contact Info

Product

Resources

About