Supercritical CO2 Extraction of Organic Solvents from Flunisolide and Fluticasone Propionate

Baldino, Lucia; Scognamiglio, Mariarosa; Reverchon, Ernesto

doi:10.3390/pharmaceutics13050612

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…Like CAPE, ARC also activated tumor suppressor activity in p53 by abrogating its complex with mortalin [ 33 ]. Several studies have demonstrated the supercritical extraction that uses high pressure, low temperature, and allows reduction in organic solvents as a preferred method of extraction for retaining sensitive natural bioactive compounds [ 34 , 35 ]. Supercritical extracts have been shown to retain aroma as well as bioactive profile that closely resembles the original source [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C

Kaul

Kuthethur

Ishida³

et al. 2021

Molecules

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Propolis, also known as bee-glue, is a resinous substance produced by honeybees from materials collected from plants they visit. It contains mixtures of wax and bee enzymes and is used by bees as a building material in their hives and by humans for different purposes in traditional healthcare practices. Although the composition of propolis has been shown to depend on its geographic location, climatic zone, and local flora; two largely studied types of propolis: (i) New Zealand and (ii) Brazilian green propolis have been shown to possess Caffeic Acid Phenethyl Ester (CAPE) and Artepillin C (ARC) as the main bioactive constituents, respectively. We have earlier reported that CAPE and ARC possess anticancer activities, mediated by abrogation of mortalin-p53 complex and reactivation of p53 tumor suppressor function. Like CAPE, Artepillin C (ARC) and the supercritical extract of green propolis (GPSE) showed potent anticancer activity. In this study, we recruited low doses of GPSE and ARC (that did not affect either cancer cell proliferation or migration) to investigate their antistress potential using in vitro cell based assays. We report that both GPSE and ARC have the capability to disaggregate metal- and heat-induced aggregated proteins. Metal-induced aggregation of GFP was reduced by fourfold in GPSE- as well as ARC-treated cells. Similarly, whereas heat-induced misfolding of luciferase protein showed 80% loss of activity, the cells treated with either GPSE or ARC showed 60–80% recovery. Furthermore, we demonstrate their pro-hypoxia (marked by the upregulation of HIF-1α) and neuro-differentiation (marked by differentiation morphology and upregulation of expression of GFAP, β-tubulin III, and MAP2). Both GPSE and ARC also offered significant protection against oxidative stress and, hence, may be useful in the treatment of old age-related brain pathologies.

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Section: Introductionmentioning

confidence: 99%

Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C

Kaul

Kuthethur

Ishida³

et al. 2021

Molecules

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“…scCO 2 is a commonly used extractant in the pharmaceutical and natural products industries [69][70][71][72][73]. It is a non-toxic, non-flammable, and environmentally friendly solvent [74,75], and it can be easily removed by depressurization and evaporation [76][77][78]. The extracts were obtained with scCO 2 at 50 • C under 2000 (a) and 6000 PSI (b) (Table 3).…”

Section: Extractionmentioning

confidence: 99%

The Antioxidant and Neuroprotective Potential of Leaves and Inflorescences Extracts of Selected Hemp Varieties Obtained with scCO2

Stasiłowicz-Krzemień,

Sip,

Szulc

et al. 2023

Antioxidants

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Cannabis sativa, a versatile plant with numerous varieties, holds promising potential for a wide range of biological activity. As raw materials for research, we chose leaves and inflorescences of hemp varieties such as Białobrzeskie, Henola, and Tygra, which are cultivated mainly for their fibers or seeds. The choice of extraction is a key step in obtaining the selected compositions of active compounds from plant material. Bearing in mind the lipophilic nature of cannabinoids, we performed supercritical carbon dioxide (scCO2) extraction at 50 °C under 2000 (a) and 6000 PSI (b). The cannabinoid contents were determined with the use of the HPLC-DAD method. The antioxidant capabilities were assessed through a series of procedures, including the DPPH, ABTS, CUPRAC, and FRAP methods. The capacity to inhibit enzymes that play a role in the progression of neurodegenerative diseases, such as acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase was also studied. The dominant cannabinoids in the extracts were cannabidiol (CBD) and cannabidiolic acid (CBDA). The highest concentration of eight cannabinoids was detected in the Tygra inflorescences extract (b). The most notable antioxidant properties were provided by the Tygra inflorescences extract (b). Nonetheless, it was the Henola inflorescences extract (b) that demonstrated the most efficient inhibition of AChE and BChE, and tyrosinase was inhibited the most significantly by the Białobrzeskie inflorescences extract (b). Multidimensional comparative analysis enrolled all assays and revealed that the Henola inflorescences extract (b) showed the most substantial neuroprotective potential.

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A critical review on the particle generation and other applications of rapid expansion of supercritical solution

Kumar

Thakur

Banerjee

et al. 2021

International Journal of Pharmaceutics

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Supercritical CO2 Extraction of Organic Solvents from Flunisolide and Fluticasone Propionate

Cited by 5 publications

References 31 publications

Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C

Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C

The Antioxidant and Neuroprotective Potential of Leaves and Inflorescences Extracts of Selected Hemp Varieties Obtained with scCO2

A critical review on the particle generation and other applications of rapid expansion of supercritical solution

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