Supercritical fluid chromatography–tandem mass spectrometry for fast bioanalysis of R/S-warfarin in human plasma

Supercritical Fluid Chromatography

“…The proposed chiral SFC/MS achieved ∼ 3 min/sample for the determination of (R, S) -propranolol at a low nanogram per milliliter level and was applied to support a pharmacokinetic study. Coe et al (2006) reported fast bioanalytical SFC/APCI -MS/MS for separation and quantifi cation of R/S -warfarin in human plasma. Compared to an LC method, the SFC method developed on Chiralpak AD column was at least two times faster.…”

Separation of Chiral Compounds on Polysaccharide Columns

“…SFC can readily be coupled to mass spectrometry detectors using either electrospray or atmospheric pressure chemical ionization (APCI) ionization. Recent applications along this line for method development [211] and drug monitoring in biological matrices have been reported ( Figure 13) [212,213]. However, of particular interest are innovative rapid screening strategies in which mixed pools of samples are evaluated with standard columns and solvents, and then detected with mass spectrometry [207].…”

Chiral separations in sub‐ and supercritical fluid chromatography

“…A paper with some practical considerations regarding the coupling of SFC with MS detection for the analysis of drugs in biological matrices was written by Coe et al [48].…”