Supercritical Fluid Extraction of Quinones from Compost for Microbial Community Analysis

Abstract

Supercritical fluid extraction (SFE) was used to extract quinones from compost to monitor the microbial community dynamics during composting. The 0.3 g of dried compost was extracted using 3 mL min −1 of carbon dioxide (90%) and methanol (10%) at 45 ∘ C and 25 MPa for a 30 min extraction time. The extracted quinones were analysed using ultra performance liquid chromatography (UPLC) with 0.3 mL min −1 of methanol mobile phase for a 50 min chromatographic run time. A comparable detected amount of quinones was obtained using the developed method and an organic solvent extraction method, being 36.06 mol kg −1 and 34.54 mol kg −1 , respectively. Significantly low value of dissimilarity index ( ) between the two methods (0.05) indicated that the quinone profile obtained by both methods was considered identical. The developed method was then applied to determine the maturity of the compost by monitoring the change of quinone during composting. The UQ-9 and MK-7 were predominant quinones in the initial stage of composting. The diversity of quinone became more complex during the cooling and maturation stages. This study showed that SFE had successfully extracted quinones from a complex matrix with simplification and rapidity of the analysis that is beneficial for routine analysis.

Penggunaan Aplikasi Blynk untuk Sistem Monitoring dan Kontrol Jarak Jauh pada Sistem Kompos Pintar berbasis IoT

“…Kualitas kompos dalam hal kematangan dan kestabilan merupakan hal utama yang harus diperhatikan dalam penggunaan kompos di lahan pertanian dan budidaya tanaman [3], karena dalam beberapa kasus penggunaan kompos yang belum matang dapat menyebabkan efek merusak, seperti keterlambatan dalam perkecambahan [4], dan menghambat proses pembuahan pada tanaman [5].…”