Supercritical fluid extraction of γ-Pyrones from Ammi visnaga L. fruits

Bishr, Mokhtar; El-Degwy, Mohamed; Hady, Mohammed Abdel; Amin, Mohamed N.; Salama, Osama

doi:10.1016/j.fjps.2017.09.001

Cited by 8 publications

(6 citation statements)

References 17 publications

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“…The components emerge from the column at different times, as Gaussian or pseudo-Gaussian peaks, and pass through a detector. As a rule of thumb, any compound that is soluble in methanol or a less polar solvent is a good candidate for separation by SFC; therefore, it is very suitable for the determination of closely related structures and complex mixtures of γ-Pyrones in A. visnaga [100]…”

Section: Supercritical Carbon Dioxide Fluid Chromatographymentioning

confidence: 99%

Ammi Visnaga L., a Potential Medicinal Plant: A Review

Khalil

Bishr²,

desouky

et al. 2020

Molecules

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Ammi visnaga L. (Visnaga daucoides Gaertn., Family Apiaceae), also known as Khella Baldi or toothpick weed, is an annual or biennial herb indigenous to the Mediterranean region of North Africa, Asia, and Europe. The plant is known to have been used in traditional medicine a long time ago. Nowadays, it is used in modern medicine to treat many aliments such as renal colic and coronary insufficiency, and is used as an antioxidant, antifungal, and antibacterial, with a larvicidal effect on mosquito larvae. Peer-reviewed studies show that these pharmacological activities are due its valuable chemical constituents that include mainly essential oil, polyphenolic compounds including flavonoids, as well as γ-pyrones, represented mainly by khellin and visnagin. Its essential oil is reported to have antiviral, antibacterial, and larvicidal effects, while its flavonoid content is responsible for its antioxidant activity. Its γ-pyrone content has a powerful effect on facilitating the passage of kidney stones and relieving renal colic, in addition to having a relaxant effect on smooth muscle including that of the coronary arteries. The current review represents the progress in research on A. visnaga in terms of either its chemistry or biological activities. This review represents scientific support material for the use of the plant by the pharmaceutical industry.

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Section: Supercritical Carbon Dioxide Fluid Chromatographymentioning

confidence: 99%

Ammi Visnaga L., a Potential Medicinal Plant: A Review

Khalil

Bishr²,

desouky

et al. 2020

Molecules

Self Cite

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“…is variation can be attributed to ecological and/or genetic factors, which in uence the plant biosynthetic pathways and consequently the relative proportion of the main common compounds [30]. e common feature of the chemical composition of khella seeds is its high prevalence of furanochromones (Khellin and Visnagin) ( Figures 1, 2), which has been the subject of several research studies [31,32].…”

Section: Identi Cation Of Compoundsmentioning

confidence: 99%

Inhibition of Mild Steel Corrosion in 1M Hydrochloric Medium by the Methanolic Extract ofAmmi visnagaL. Lam Seeds

Zaher

Chaouiki

Salghi

et al. 2020

International Journal of Corrosion

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The chemical composition of the methanolic extract of Ammi visnaga (Khella) seeds from the Sidi Slimane region is determined for the first time by Gas Chromatography coupled with Mass Spectrometry (GC/MS). Ten compounds representing 99.638% of the total extract were identified. Khellin (49.011%), Visnagin (26.537%) and Dimethylethylamine (15.108%) are the major components. Moreover, the inhibitory effect of the Methanolic extract of the seeds of Ammi visnaga on the corrosion of mild steel in a solution of 1M HCl is determined using weight loss measurements, the potentiodynamic technique as well as the technique of electrochemical impedance spectroscopy (EIS). It is found that the extract reduces the corrosion rate of the steel in the acid solution. Inhibition efficiency increases as the concentration of the extract increases. The tested compound has an inhibition efficiency of 84% for a concentration equal to 1.0 g/L. The polarization measurements indicate that the examined extract acts as a mixed inhibitor with predominant anodic efficacy. The data obtained from EIS studies are analyzed to model this process using appropriate equivalent circuit models. The adsorption of the extract on the surface of the mild steel obeys the Langmuir adsorption isotherm in acidic medium and the activation is determined and discussed.

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“…Ten kilograms of finely ground air-dried A. visnaga fruits (with particle size 1 mm) were packed in 20 L extraction vessel of 20 L ASI pilot scale SCFE unit (Applied Separations, Inc. Allentown, PA, USA), which is schematically presented in Figure 2 . Extraction was carried out according to previously validated extraction conditions [ 40 ] The extraction was started with static time of 2 h, followed by dynamic extraction for another 2 h with flow rate of supercritical fluid carbon dioxide (containing 5% methanol as co-solvent) 2 L/min, supercritical pressure was 200 Bar and supercritical temperature of 45 °C.…”

Section: Methodsmentioning

confidence: 99%

Assessment of Conventional Solvent Extraction vs. Supercritical Fluid Extraction of Khella (Ammi visnaga L.) Furanochromones and Their Cytotoxicity

Khalil

Bishr²,

El-Degwy³

et al. 2021

Molecules

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Background: Khella (Ammi visnaga Lam.) fruits (Apiaceae) are rich in furanochromones, mainly khellin and visnagin, and are thus incorporated in several pharmaceutical products used mainly for treatment of renal stones. Methods: The objective of this study was to compare the yield of khellin and visnagin obtained using different conventional solvents and supercritical fluid extraction (SCFE) with carbon dioxide (containing 5% methanol as co-solvent). Water, acetone and ethanol (30% and 95%) were selected as conventional solvents. Results: Highest extract yield was obtained from 30% ethanol (15.44%), while SCFE gave the lowest yield (4.50%). However, the percentage of furanochromones were highest in SCFE (30.1%), and lowest in boiling water extract (5.95%). HPLC analysis of conventional solvent extracts showed other coumarins that did not appear in supercritical fluid extraction chromatogram due to non-selectivity of solvent extraction. Ammi visnaga extracts as well as standard khellin and visnagin were tested for their cytotoxic activity using sulforhodamine B assay on breast cancer (MCF-7) and hepatocellular carcinoma (Hep G2) cell lines. Results revealed a strong cytotoxic activity (IC50 < 20 µg/mL) for the SCFE and standard compounds (khellin and visnagin) (IC50 ranging between 12.54 ± 0.57 and 17.53 ± 1.03 µg/mL). However, ethanol and acetone extracts had moderate cytotoxic activity (IC50 20–90 µg/mL) and aqueous extract had a weak activity (IC50 > 90 µg/mL). Conclusions: Thus, supercritical fluid extraction is an efficient, relatively safe, and cheap technique that yielded a more selective purified extract with better cytotoxic activity.

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Supercritical fluid extraction of γ-Pyrones from Ammi visnaga L. fruits

Cited by 8 publications

References 17 publications

Ammi Visnaga L., a Potential Medicinal Plant: A Review

Ammi Visnaga L., a Potential Medicinal Plant: A Review

Inhibition of Mild Steel Corrosion in 1M Hydrochloric Medium by the Methanolic Extract ofAmmi visnagaL. Lam Seeds

Assessment of Conventional Solvent Extraction vs. Supercritical Fluid Extraction of Khella (Ammi visnaga L.) Furanochromones and Their Cytotoxicity

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