Supercritical fluid extractive fractionation – study of the antioxidant activities of propolis

Wang, Be-Jen; Lien, Yen-Hui; Yu, Zer‐Ran

doi:10.1016/j.foodchem.2003.09.031

Cited by 120 publications

(81 citation statements)

References 31 publications

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“…It was found that metal chelating activity of EEP was around 90% for the concentration of 25 mg/mL and more. Metal chelating activity of different propolis extracts have been reported as 2 mg/mL has showed around 50% inhibition from Brazilian 35 , 70% inhibition for the concentration of 2 mg/mL from Malatya, Turkey 33 and 2 mg/mL has showed around 55.9% inhibition from Slovenian. 36 Study of Miguel et al reported that chelating Fe 2+ ions activity of methanolic propolis extract has from plant samples directly without compromising the structure.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Properties and Protective Effect of Turkish Propolis on t-BHP-Induced Oxidative Stress in Foreskin Fibroblast Cells

Mısır¹,

Aliyazıcıoğlu²,

Demir³

et al. 2018

IJPER

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Objective: Propolis is an important bee product, rich in polyphenolic compounds. It has antitumoral, antioxidant, antimutagenic, and other useful activities. Biological activities of propolis are generally attributed to its substance of polyphenolic compounds. The aim of this study was to investigate the in vitro antioxidant properties and reduction amount of intracellular reactive oxygen species (ROS) in human normal foreskin fibroblast cells by Turkish propolis ethanolic extract (EEP). Method: Total phenolic and flavonoid contents, reducing power, radical scavenging capacity, and iron chelating activity of EEP were determined using spectrophotometric methods. Intracellular ROS levels were determined by spectrofluorometric analysis with CM-H 2 DCFDA. Results: Our results indicated that antioxidant activity was correlated with the higher total phenolic and flavonoid contents of EEP. The generated ROS levels were significantly decreased by EEP compared to only t-BHP group. Conclusion: Ethanolic propolis extract may be evaluated as a potency antioxidant resource and/or a novel natural agent in food and drug industries.

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Section: Discussionmentioning

confidence: 99%

Antioxidant Properties and Protective Effect of Turkish Propolis on t-BHP-Induced Oxidative Stress in Foreskin Fibroblast Cells

Mısır¹,

Aliyazıcıoğlu²,

Demir³

et al. 2018

IJPER

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“…Propolis, especially its compound CAPE (Caffeic Acid Phenethyl Ester), is used in research on heat stress mitigation, as it improves thermal tolerance (Chen et al, 2009). The antioxidant, cytostatic, antimutagenic, and immunomodulatory properties of propolis are based on its rich flavonoid, phenolic acid, and terpenoid contents (Kimoto et al, 1999;Wang et al, 2003). Current literature indicates that the inclusion of propolis in poultry diets might improve their immune status without influencing their weight gain or performance (Khojasteh & Shivazad, 2006;Seven, 2008).…”

Section: Introductionmentioning

confidence: 99%

Productive, Qualitative, and Physiological Aspects of Layer Hens Fed with Propolis

Belloni

Paz

Nääs

et al. 2015

Rev. Bras. Cienc. Avic.

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Egg production, infrared thermography, natural diet supplement.Submitted: September/2014 Approved: March/2015 ABSTRACT Laying hens reared under tropical conditions are usually under heat stress. Propolis is known for its pharmaceutical properties, such as increasing cell tolerance to hyperthermia, because of its antioxidants effects. This study aimed at evaluating the influence of different dietary propolis inclusion levels on the performance, egg quality, and bird surface temperature of layers. In this experiment 120 55-wk-old Isa Brown ® layers were distributed according to a completely randomized experimental design into four treatments (0, 1, 2 and 3% dietary propolis inclusion levels), with three replicates of ten birds each. Performance and egg quality parameters, and birds' surface temperature were evaluated. Egg production, egg mass, feed intake, and feed conversion ratio were influenced by the treatments. Bird surface temperature was not affected by propolis dietary inclusion. The egg yolk color changed with the treatment (p<0.05) when brightness and red and yellow concentration were considered. Evaluators noted a slight difference among treatments during the sensory analysis. The use of propolis in the hens' diet did not improve performance and worsened the eggs' quality.

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“…Supercritical carbon dioxide (sc-CO2) has also been applied to propolis, yielding highly valuable products (Catchpole et al, 2004;Wang and Yu et al, 2004). Raw propolis was extracted by Stahl et al (1988) using supercritical CO 2 at 600 bar and 40°C to extract the wax and leave the insoluble flavonoids behind.…”

Section: Introductionmentioning

confidence: 99%

Supercritical CO2 extraction of raw propolis and its dry ethanolic extract

Paviani

Saito

Dariva

et al. 2012

Braz. J. Chem. Eng.

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-Three types of propolis extract were prepared and analyzed with respect to their global extraction yields and with respect to the concentration of the following markers: 3,5-diprenyl-4-hydroxycinnamic acid; 3-prenyl-4-hydroxycinnamic acid; 4-hydroxycinnamic acid and 4-methoxy-3,5,7-trihydroxyflavone. The extract EEP (ethanolic extract of propolis) was obtained by the conventional method from raw propolis using ethanol as solvent. The extracts (SFE) were obtained by supercritical solvent extraction from the raw propolis using supercritical carbon dioxide (sc-CO 2 ), with and without the addition of ethanol as a co-solvent. The fractionated supercritical extracts (FSCE) were obtained by fractionation (extract and raffinate) of the dry EEP with sc-CO 2 . EEP yields of 39.5% were obtained and maximum global extraction yields were 7.3% for SFE with no co-solvent, 51% for SFE with 15% ethanol and 18% for the FSCE extract fraction. The concentrations of the markers in the different extracts differed as a function of the operational parameters, indicating that the addition of co-solvent and the selectivity of sc-CO 2 could be manipulated so as to obtain extracts with the yields and concentrations of interest.

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Supercritical fluid extractive fractionation – study of the antioxidant activities of propolis

Cited by 120 publications

References 31 publications

Antioxidant Properties and Protective Effect of Turkish Propolis on t-BHP-Induced Oxidative Stress in Foreskin Fibroblast Cells

Antioxidant Properties and Protective Effect of Turkish Propolis on t-BHP-Induced Oxidative Stress in Foreskin Fibroblast Cells

Productive, Qualitative, and Physiological Aspects of Layer Hens Fed with Propolis

Supercritical CO2 extraction of raw propolis and its dry ethanolic extract

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