Superficial NK1 expressing spinal dorsal horn neurones modulate inhibitory neurotransmission mediated by spinal GABAA receptors

Rahman, Wahida; Sikander, S; Suzuki, Rie; Hunt, Stephen P.; Dickenson, Anthony H.

doi:10.1016/j.neulet.2007.04.039

Cited by 13 publications

(9 citation statements)

References 32 publications

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“…Any toxin not receptor bound is subject to prompt degradation by neutral endopeptidases, at the doses used in these experiments. Lumbar intrathecal nonconjugated saporin (Rahman et al, 2007;Vera-Portocarrero et al, 2007) or blank-saporin (Abe et al, 2005) had no measurable anatomical or behavioral effects, an observation previously confirmed on numerous occasions in our laboratory for intrathecal saporin doses Յ500 ng. To confirm the lack of effect of lumbar intrathecal unconjugated saporin, we determined the effects of lumbar intrathecal saporin on dorsal horn MOR staining.…”

Section: Dorsal Horn Anatomysupporting

confidence: 79%

Spinal μ-Opioid Receptor-Expressing Dorsal Horn Neurons: Role in Nociception and Morphine Antinociception

Kline¹,

Wiley²

2008

J. Neurosci.

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The role of spinal cord -opioid receptor (MOR)-expressing dorsal horn neurons in nociception and morphine analgesia is incompletely understood. Using intrathecal dermorphin-saporin (Derm-sap) to selectively destroy MOR-expressing dorsal horn neurons, we sought to determine the role of these neurons in (1) normal baseline reflex nocifensive responses to noxious thermal stimulation (hotplate, tail flick) and to persistent noxious chemical stimulation (formalin) and (2) the antinociceptive activity of intrathecal and systemic morphine in the same tests. Lumbar intrathecal Derm-sap (500 ng) produced (1) partial loss of lamina II MOR-expressing dorsal horn neurons, (2) no effect on MOR-expressing dorsal root ganglion neurons, and (3) no change in baseline tail-flick and hotplate reflex nocifensive responses. Derm-sap treatment attenuated the antinociceptive action of both intrathecal and systemic morphine on hotplate responses. Derm-sap treatment had two effects in the formalin test: (1) increased baseline nocifensive responding and (2) reduced antinociceptive action of systemic morphine. We conclude that MOR-expressing dorsal horn neurons (1) are not essential for determining nocifensive reflex responsiveness to noxious thermal stimuli, (2) are necessary for full antinociceptive action of morphine (intrathecal or systemic) in these tests, and (3) play a significant role in the endogenous modulation of reflex nocifensive responses to persistent pain in the formalin test. Thus, one would predict that altering the activity of MOR-expressing dorsal horn neurons would be antinociceptive and of interest in the search for new approaches to management of chronic pain.

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Section: Dorsal Horn Anatomysupporting

confidence: 79%

Spinal μ-Opioid Receptor-Expressing Dorsal Horn Neurons: Role in Nociception and Morphine Antinociception

Kline¹,

Wiley²

2008

J. Neurosci.

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“…29 Briefly, mechanical sensitivity was assessed through measurement of foot withdrawal frequencies to a trial of 10 applications of calibrated von Frey filaments 2 and 8 g to each hind paw. Behavioral hypersensitivity to a cooling stimulus was similarly assessed as the number of withdrawals out of a trial of 5 applications of acetone.…”

Section: Mechanical and Cooling Sensitivitymentioning

confidence: 99%

Peripheral Nerve Injury–Induced Changes in Spinal α2-Adrenoceptor–Mediated Modulation of Mechanically Evoked Dorsal Horn Neuronal Responses

Rahman

D’Mello

Dickenson

2008

The Journal of Pain

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“…Many studies have shown that a large amount of pain-associated molecules act in the dorsal spinal cord, especially those associated with nerve pathological pain (Quan-Xin et al 2012;Wei et al 2013;Xie et al 2012;Zhu et al 2013). Besides, various neurotransmitters and neurotransmitter receptors in the dorsal spinal cord could be altered during the nerve pathological pain (Paul et al 2012;Rahman et al 2007;Wan et al 2004). Since incisional pain following thoracic operation has been reported to be among the most intense cases, the aim of this study was to establish the post-chronic thoracotomy pain model and compare protein expression in the dorsal spinal cord with or without exposure to chronic pain by using differential proteomics.…”

Section: Introductionmentioning

confidence: 99%

Glutaminase 1 is a potential biomarker for chronic post-surgical pain in the rat dorsal spinal cord using differential proteomics

et al. 2015

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Chronic post-surgical pain (CPSP) is a normal and significant symptom in clinical surgery, such as breast operation, biliary tract operation, cesarean operation, uterectomy and thoracic operation. Severe chronic post-surgical pain could increase post-surgical complications, including myocardial ischemia, respiratory insufficiency, pneumonia and thromboembolism. However, the underlying mechanism is still unknown. Herein, a rat CPSP model was produced via thoracotomy. After surgery, in an initial study, 5 out of 12 rats after surgery showed a significant decrease in mechanical withdrawal threshold and/or increase in the number of acetone-evoked responses, and therefore classified as the CPSP group. The remaining seven animals were classified as non-CPSP. Subsequently, open-chest operation was performed on another 30 rats and divided into CPSP and non-CPSP groups after 21-day observation. Protein expression levels in the dorsal spinal cord tissue were determined by 12.5 % SDS-PAGE. Finally, differently expressed proteins were identified by LC MS/MS and analyzed by MASCOT software, followed by Gene Ontology cluster analysis using PANTHER software. Compared with the non-CPSP group, 24 proteins were only expressed in the CPSP group and another 23 proteins expressed differentially between CPSP and non-CPSP group. Western blot further confirmed that the expression of glutaminase 1 (GLS1) was significantly higher in the CPSP than in the non-CPSP group. This study provided a new strategy to identify the spinal proteins, which may contribute to the development of chronic pain using differential proteomics, and suggested that GLS1 may serve as a potential biomarker for CPSP.

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Superficial NK1 expressing spinal dorsal horn neurones modulate inhibitory neurotransmission mediated by spinal GABAA receptors

Cited by 13 publications

References 32 publications

Spinal μ-Opioid Receptor-Expressing Dorsal Horn Neurons: Role in Nociception and Morphine Antinociception

Spinal μ-Opioid Receptor-Expressing Dorsal Horn Neurons: Role in Nociception and Morphine Antinociception

Peripheral Nerve Injury–Induced Changes in Spinal α2-Adrenoceptor–Mediated Modulation of Mechanically Evoked Dorsal Horn Neuronal Responses

Glutaminase 1 is a potential biomarker for chronic post-surgical pain in the rat dorsal spinal cord using differential proteomics

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