Superhydrophobic surfaces (SHSs) have the potential to achieve large drag reduction for internal and external flow applications. However, experiments have shown inconsistent results, with many studies reporting significantly reduced performance. Recently, it has been proposed that surfactants, ubiquitous in flow applications, could be responsible by creating adverse Marangoni stresses. However, testing this hypothesis is challenging. Careful experiments with purified water already show large interfacial stresses and, paradoxically, adding surfactants yields barely measurable drag increases. To test the surfactant hypothesis while controlling surfactant concentrations with precision higher than can be achieved experimentally, we perform simulations inclusive of surfactant kinetics. These reveal that surfactant-induced stresses are significant at extremely low concentrations, potentially yielding a no-slip boundary condition on the air-water interface (the "plastron") for surfactant concentrations below typical environmental values. These stresses decrease as the stream-wise distance between plastron stagnation points increases. We perform microchannel experiments with SHSs consisting of streamwise parallel gratings, which confirm this numerical prediction, while showing near-plastron velocities significantly slower than standard surfactant-free predictions. In addition, we introduce an unsteady test of surfactant effects. When we rapidly remove the driving pressure following a loading phase, a backflow develops at the plastron, which can only be explained by surfactant gradients formed in the loading phase. This demonstrates the significance of surfactants in deteriorating drag reduction and thus the importance of including surfactant stresses in SHS models. Our time-dependent protocol can assess the impact of surfactants in SHS testing and guide future mitigating designs.superhydrophobic surface | drag reduction | surfactant | Marangoni stress | plastron S uperhydrophobic surfaces (SHSs) combine hydrophobic surface chemistry and micro-or nanoscale patterning to retain a network of air pockets when exposed to a liquid (e.g., reviews in refs. 1-3). Because a large portion of the interface between the solid wall and the liquid is replaced by an air-liquid interface, which can be considered almost as a shear-free surface (known as a "plastron"), SHSs could be used to obtain significant drag reduction in fluid flow applications (4, 5). Microchannel tests have recorded drag reductions of over 20% (e.g., refs. 6-11) and rheometer tests reported slip lengths of up to 185 µm (12). Turbulent flow experiments have reduced drag by up to 75% (13-16). However, a wide range of experiments have provided inconsistent results, with several studies reporting little or no drag reduction (16)(17)(18)(19)(20)(21)(22)(23)(24)(25).A key step toward solving this puzzle has come with the realization that surfactants could induce Marangoni stresses that impair drag reduction. This was first hypothesized to account for experiments that rev...