2023
DOI: 10.1038/s41598-023-44676-5
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Superior performance of biofilm versus planktonic Limosilactobacillus reuteri in protection of the intestines and brain in a piglet model of necrotizing enterocolitis

Samantha J. Wala,
Nitin Sajankila,
Mecklin V. Ragan
et al.

Abstract: Necrotizing enterocolitis (NEC) is the leading cause of gastrointestinal-related death in premature infants. Its etiology is multifactorial, with intestinal dysbiosis playing a major role. Probiotics are a logical preventative therapy for NEC, however their benefits have been inconsistent. We previously developed a novel probiotic delivery system in which planktonic (free-living) Limosilactobacillus reuteri (Lr) is incubated with biocompatible dextranomer microspheres (DM) loaded with maltose (Lr-DM-maltose) t… Show more

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Cited by 3 publications
(3 citation statements)
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“…a spectrum from hyperemia to necrosis). The 6-grade system has been used extensively in preterm piglet studies and was scored as follows: 1 = normal appearance; 2 = hyperemia; 3 = local hemorrhage; 4 = extensive hemorrhage; 5 = local necrosis and/or pneumatosis intestinalis, and 6 = extensive necrosis and/or pneumatosis intestinalis [8,11,12]. For the systematic, descriptive method, the presence of hyperemia, hemorrhage, pneumatosis intestinalis, and necrosis were registered separately.…”
Section: Post-mortem Macroscopic Lesion Scoring and Tissue Collectionmentioning
confidence: 99%
“…a spectrum from hyperemia to necrosis). The 6-grade system has been used extensively in preterm piglet studies and was scored as follows: 1 = normal appearance; 2 = hyperemia; 3 = local hemorrhage; 4 = extensive hemorrhage; 5 = local necrosis and/or pneumatosis intestinalis, and 6 = extensive necrosis and/or pneumatosis intestinalis [8,11,12]. For the systematic, descriptive method, the presence of hyperemia, hemorrhage, pneumatosis intestinalis, and necrosis were registered separately.…”
Section: Post-mortem Macroscopic Lesion Scoring and Tissue Collectionmentioning
confidence: 99%
“…In a piglet model of necrotizing small intestinal colitis, enteral administration of Lr in the biofilm state exhibited positive effects on the gut, microbiome, and developing brain, with a greater impact on necrotizing enterocolitis (NEC) compared to Lr in the planktonic state. 313 It could be postulated that biofilm delivery, even without live bacteria, may fulfill similar functions as probiotics, as many of their functionalities are mediated by surface properties and structure. Nevertheless, further research is warranted to validate this hypothesis.…”
Section: Nanosystems Based On Metabolites Of the Gut Microbiotamentioning
confidence: 99%
“…Gail E. Besner conducted an experiment where L. rhamnosus (Lr) was incubated with biocompatible dextran microspheres (DM) loaded with maltose to induce biofilm formation. In a piglet model of necrotizing small intestinal colitis, enteral administration of Lr in the biofilm state exhibited positive effects on the gut, microbiome, and developing brain, with a greater impact on necrotizing enterocolitis (NEC) compared to Lr in the planktonic state . It could be postulated that biofilm delivery, even without live bacteria, may fulfill similar functions as probiotics, as many of their functionalities are mediated by surface properties and structure.…”
Section: Representative Applications Of Bioinspired Therapeutic Platf...mentioning
confidence: 99%