Supernatants from quartz dust treated human macrophages stimulate cell proliferation of different human lung cells as well as collagen-synthesis of human diploid lung fibroblasts in vitro

New anti-fibrotic mechanisms of n-acetyl-seryl-aspartyl-lysyl-proline in silicon dioxide-induced silicosis

“…Olbrück et al had reported that the conditioned medium from human macrophages contained TGF-β1 (Olbrück et al 1998). Using a quartz-induced silicosis model, Chen et al also found that TGF-β1 expression was increased in the lung (Chen et al 2005).…”

“…The best characterized of these cytokines is TGF-β1 (Olbrück et al 1998;Wang et al 2009a, b). In order to determine whether Ac-SDKP can suppress TGF-β1 production in SiO 2 -induced rats, we test the level of TGF-β1 protein by western blot.…”

“…Among them, transforming growth factor-β (TGF-β) appears to be important. It has been reported that macrophages stimulated by quartz dust are able to synthesize TGF-β1 which is responsible for collagen synthesis of human lung fibroblasts and proliferation (Olbrück et al 1998;Ji et al 2009). By contrast, inactivation of TGF-β1 by silencing expression of CD36 in macrophages significantly reduces collagen production and prevents the development of silica-induced lung fibrosis (Wang et al 2009a,b).…”

The Role of Transforming Growth Factor β in Lung Development and Disease

“…Antibodies to TGF␤1 also decreased fibrogenesis by human lung fibroblasts in vitro. 139 In the mouse epithelial transfer of the decorin gene, a naturally occurring TGF␤ inhibitor that binds and neutralizes TGF␤ was also able to prevent TGF␤-induced inhibition of normal lung development. 140 In addition, TGF␤-induced antiproliferative effects as well as the down-regulation of surfactant protein C were abrogated by decorin in cultured embryonic lungs, and lung branching inhibition by TGF␤ could be restored by the addition of decorin in culture.…”

IL-1β as a determinant in silica-induced cytokine responses in monocyte-endothelial cell co-cultures

“…3 Alveolar macrophages (AM) together with lung epithelial cells are the first target cells for respirable particles. 4 Especially AM seem to play an important role in initiating the inflammatory response and in co-ordinating alveolar remodelling. 5,6 Prolonged exposure of the lung to crystalline silica may lead to an incomplete lung epithelial repair showing areas of denuded basement membrane.…”