Superovulation alters DNA methyltransferase protein expression in mouse oocytes and early embryos

Uysal, Fatma; Öztürk, Saffet; Akkoyunlu, Gökhan

doi:10.1007/s10815-017-1087-z

Cited by 40 publications

(46 citation statements)

References 44 publications

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“…Indeed, some studies have reported increased epigenetic anomalies in children born after assisted reproductive technologies (ARTs; Lawrence, 2008) and that the overgrowth found in calves and lambs born after these technologies uses could be caused by methylations fails (Sinclair, Young, Wilmut, & McEvoy, ; Young et al, ). ARTs commonly use superovulation protocols that decreased DNMTs protein expression in oocytes (Uysal, Ozturk, & Akkoyunlu, ). Oocyte imprints seems to be established in later oocyte development stages (Lucifero, Mertineit, Clarke, Bestor, & Trasler, ; Obata & Kono, ; Obata et al, ; Tada et al, ) which could be linked with the higher expression previous found of these three DNMTs in mouse (Uysal, Ozturk, & Akkoyunlu, ) and bovine oocytes (O'Doherty, O'Shea, & Fair, ).…”

Section: Discussionmentioning

confidence: 99%

Equine chorionic gonadotropin drives the transcriptional profile of immature cumulus‐oocyte complexes and in vitro‐produced blastocysts of superstimulated Nelore cows

Franchi

Satrapa

Fontes

et al. 2019

Molecular Reproduction Devel

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Studies have shown that the use of equine chorionic gonadotropin (eCG), which binds both follicle stimulating hormone (FSH) and luteinizing hormone (LH) receptors, could modify the female reproductive tract. We, thus, aimed to quantify the messenger RNA (mRNA) abundance of genes related to cumulus‐oocyte complexes (COCs) and embryo quality in Nelore cows (Bos taurus indicus) submitted to ovarian superstimulation using only FSH (FSH group; n = 10) or replacement of the last two doses of FSH by eCG (FSH/eCG group; n = 10). All animals were slaughtered and the ovarian antral follicles from both groups (10–14 mm in diameter) were aspirated for cumulus, oocyte and in vitro embryo production gene expression analysis. The relative mRNA abundance of 96 genes related to COCs development and embryo quality was measured by RT‐qPCR. We found that oocytes are more affected by eCG use and that 35 genes involved in lipid metabolism, oxidative stress, transcriptional control, and cellular development were upregulated in the FSH/eCG group. In blastocysts, lipid metabolism seems to be the main pathway regulated by eCG use. We suggest that these multiple effects could be due to the ability of eCG to bind LHR and FSHR, which could activate multiple signal transduction pathways in the superstimulated ovary, further impacting the transcriptional profile of COCs and blastocysts.

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Section: Discussionmentioning

confidence: 99%

Equine chorionic gonadotropin drives the transcriptional profile of immature cumulus‐oocyte complexes and in vitro‐produced blastocysts of superstimulated Nelore cows

Franchi

Satrapa

Fontes

et al. 2019

Molecular Reproduction Devel

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“…T A B L E 5 Total cell numbers and their allocation to the ICM and TE lineages of yak blastocysts from the optimum concentration FGF10 groups (5.0 ng/ml). Ohnami et al 2012;Uysal et al 2017aUysal et al , 2017b. However, the functions of these genes have been studied by various approaches, and the obtained results differ notably (Antalíková et al 2015 was significantly increased in the group treated with 5 ng/ml FGF10 during both IVM and capacitation (Table 5 and Figures 6 and 8).…”

Section: Discussionmentioning

confidence: 96%

“…DNA methylation is an important epigenetic mechanism and has been implicated in playing critical roles during gametogenesis, fertilization, and preimplantation embryo development, helping in the regulation of the expression of development-related genes and establishing maternal and paternal imprints in a timely manner (Bartolomei & Fergusonsmith, 2011;Tanigawa et al, 2008;Uysal, Ozturk, & Akkoyunlu, 2017a, 2017bD. Zhang et al, 2018).…”

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confidence: 99%

FGF10 enhances yak oocyte fertilization competence and subsequent blastocyst quality and regulates the levels of CD9, CD81, DNMT1, and DNMT3B

Wang

Baloch

Zhang

et al. 2019

Journal Cellular Physiology

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The fusion of sperm and oocytes determines the fertilization competence and subsequent development of embryos, which, in turn, can be affected by various proteins and DNA methylation. However, several factors in this whole regulation process remain unknown, especially in yaks. Here, we report that fibroblast growth factor 10 (FGF10) is an important growth factor that can enhance the maturation rate of yak oocytes and the motility of frozen spermatozoa. Subsequent blastocyst quality was also improved by increasing the total cell number and level of pregnancy‐associated protein in blastocysts. These effects were significantly high in the group that received the 5 ng/ml FGF10 treatment, during both in vitro maturation (IVM) and capacitation. Our data show that the effects of FGF10 were dose‐dependent at vital steps of embryogenesis in vitro. Furthermore, quantitative polymerase chain reaction, western blot analysis, and immunofluorescence demonstrated that the levels of CD9, CD81, DNMT1, and DNMT3B in both mature cumulus‐oocyte complexes and capacitated sperms were regulated by FGF10, which was also highly expressed in the group treated with 5 ng/ml FGF10 during both IVM and capacitation. From our present study, we concluded that FGF10 promotes yak oocyte fertilization competence and subsequent blastocyst quality, and could also regulate CD9, CD81, DNMT1, and DNMT3B to optimize sperm–oocyte interactions and DNA methylation during fertilization.

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“…In ovarian superstimulation, various studies suggest that the acquisition of imprinting patterns in the oocyte might be perturbed and lead to abnormal allelic expression in later embryo and placenta development (reviewed in Anckaert et al, 2013;McGraw and Trasler, 2013). However, studies show that superovulation treatments do not alter normal imprinted methylation acquisition in oocytes, but rather disrupt maternal-effect gene products that are required during pre-implantation for imprint maintenance (Denomme et al, 2011;Uysal et al, 2018). We also showed that some of these induced errors of imprinted gene expression (H19, Igf2) present in mid-gestation mouse placenta are no longer apparent at the end of the gestation (Fortier et al, 2008;Fortier et al, 2014).…”

Section: Art Proceduresmentioning

confidence: 99%

DNA methylation, environmental exposures and early embryo development

2019

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The first crucial step in the developmental program occurs during pre-implantation, the time after the oocyte has been fertilized and before the embryo implants in the uterus. This period represents a vulnerable window as the epigenome undergoes dynamic changes in DNA methylation profiles. Alterations in the early embryonic reprogramming wave can impair DNA methylation patterns and induce permanent changes to the developmental program, leading to the onset of adverse health outcomes in offspring. Although there is an increasing body of evidence indicating that harmful exposures during preimplantation embryo development can trigger lasting epigenetic alterations in offspring, the mechanisms are still not fully understood. Since physiological or pathological changes in DNA methylation can occur as a response to environmental cues, proper environmental milieu plays a critical role in the success of embryonic development. In this review, we depict the mechanisms behind the embryonic epigenetic reprogramming of DNA methylation and highlight how maternal environmental stressors (e.g., alcohol, heat stress, nutrient availability) during pre-implantation and assisted reproductive technology procedures affect development and DNA methylation marks.

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Superovulation alters DNA methyltransferase protein expression in mouse oocytes and early embryos

Abstract: This study has demonstrated for the first time that superovulation alters expression levels of the DNMT proteins, a finding that indicates that certain developmental defects in superovulated oocytes and early embryos may result from impaired DNA methylation processes.

Cited by 40 publications

References 44 publications

Equine chorionic gonadotropin drives the transcriptional profile of immature cumulus‐oocyte complexes and in vitro‐produced blastocysts of superstimulated Nelore cows

Equine chorionic gonadotropin drives the transcriptional profile of immature cumulus‐oocyte complexes and in vitro‐produced blastocysts of superstimulated Nelore cows

FGF10 enhances yak oocyte fertilization competence and subsequent blastocyst quality and regulates the levels of CD9, CD81, DNMT1, and DNMT3B

DNA methylation, environmental exposures and early embryo development

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