Superovulation and expression of follicle-stimulating hormone receptor in young rabbit females

Abstract

<p>To optimise the use of juvenile in vitro embryo transfer technologies in young rabbit females, superovulation was performed in New Zealand White young rabbit females at different ages and the expression mode of follicle-stimulating hormone receptor (FSHR) was explored using real-time quantitative polymerase chain reaction, and in vitro maturation (IVM) together with fertilisation (IVF) was conducted immediately after superovulation. The results showed that (1) the age factor significantly affected superovulation in young rabbit females, with 60 d as an optimal age; (2) the mRNA level of FSHR exhibited a rising trend, though it was lower at 30 to 40 d of age; (3) the maturation rate of the oocytes from 60 d old rabbits was significantly higher than in those from 50 d old rabbits; (4) the fertilisation rate of oocytes was not significantly different among rabbits 50, 60 and 70 d old.</p>

Oxidative Effects of Potassium Dichromate on Biochemical, Hematological Characteristics, and Hormonal Levels in Rabbit Doe (Oryctolagus cuniculus)

“…Animals in this study are of the same age and in principle age is not therefore a factor of variation in hormonal level in this case. Considering other studies, such as that carried out by Zhang et al [53] in New Zeeland female rabbits and Labib et al [54], age and corpus luteum formation can be a factor of hormonal expression.…”

Three‐dimensional in vitro maturation of rabbit oocytes enriched with sheep decellularized greater omentum

“…The culture medium for IVM is considered a key element in the success rate of this technique. In the present study, Ham's F10 medium was used for the first time in rabbit oocyte culture; other studies have used TCM‐199 (Arias‐Álvarez et al., 2018; Zhang et al., 2017). Ham's F10 medium is not as rich and nutritious as the TCM‐199 culture medium.…”

“…Subsequent studies have shown successful ovulation by intravenous administration of HCG and intramuscular administration of GnRH (Molina et al., 1987). In most studies on IVM of rabbit oocytes, superovulation has been performed (Viudes‐de‐Castro et al., 2017) by injection of hormonal drugs such as HCG (Izumi et al., 2013), FSH (Zhang et al., 2017), and PMSG (Izumi et al., 2013) to recover the COCs. Unfortunately, superovulation in humans has many serious side effects, such as ovarian hyperstimulation syndrome.…”

“…The rabbit oocytes' biochemical and physiological processes have close similarities to humans, so it can be considered an important experimental model to study gamete and embryo engineering (Tao et al., 2008). Superovulation using gonadotropin is a process that induces an increase in the number of oocytes in infertile females (Zhang et al., 2017). This process is abundantly used in rabbit models (Zhang et al., 2017).…”

“…Superovulation using gonadotropin is a process that induces an increase in the number of oocytes in infertile females (Zhang et al., 2017). This process is abundantly used in rabbit models (Zhang et al., 2017). One of the main challenges in assisted reproductive technologies (ART) is avoiding the risk of the ovarian hyperstimulation syndrome (OHSS) due to gonadotropin.…”