Superovulatory and embryo yielding in sheep using increased exposure time to progesterone associated with a GnRH agonist

Brasil, Oscar Oliveira; Moreira, N. H.; Santos, Guilherme; Silva, B.D.M.; Mariante, A. da S.; Ramos, A. F.

doi:10.1016/j.smallrumres.2016.01.005

Cited by 19 publications

(23 citation statements)

References 20 publications

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“…Percentage of ewes presenting estrus in the Day 0 protocol was higher than reported by Lehloenya and Greyling (Lehloenya and Greyling 2010), who also used Day 0 protocol in sheep and achieved 71.4% estrus percentage. However, our data agree with Brasil et al (2016), who worked with CIDR for 14 days in hair sheep (Santa Ines breed) and also achieved 100% females in estrus. In addition, our results are consistent with Menchaca et al (2007), who compared the Day 0 and Traditional protocols in sheep and obtained 100% and 95.5% females in estrus after Traditional and Day 0 protocols, respectively.…”

Section: Discussionsupporting

confidence: 92%

“…The ovary showed the large follicles absence (>4 mm) probably by the small follicles in the ovary, which promoted a higher follicular recruitment with a homogeneous pool of growing small follicles. Brasil et al (2016) suggested that a longer exposure time to progesterone associated with GnRH increased the fertilization rate. According to these authors, the greater fertilization is due to the greater synchronization of ovulation.…”

Section: Discussionmentioning

confidence: 99%

“…The MOET (multiple ovulation and embryo transfer) technique is an important tool as it accelerates and produces greater precision in the animal selection process (Brasil et al 2016). Currently, it is known that the ovarian response to superovulatory treatments is related to the ovarian follicular status at the start of the treatment with follicle-stimulating hormone (FSH).…”

Section: Introductionmentioning

confidence: 99%

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Comparison between Day 0 and traditional protocols for estrus synchronization and multiple ovulation in crossbred hair sheep

Magalhães

Júnior

Guimarães

et al. 2019

Journal of Applied Animal Research

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This study was carried out to compare Day 0 and traditional protocols for in vivo embryo production. Twenty-two crossbred ewes were used as embryo donors and assigned to two groups. Ewes from Traditional protocol were submitted to estrus synchronization with CIDR for 14 days and superovulation treatment was induced 60 h before the withdrawal of CIDR with 240 mg pFSH. Donors from Day 0 protocol were subjected to a short-term protocol of estrus synchronization (6 days) and superovulation treatment was the same to the other group, but it started 84 h after CIDR withdrawal. Embryo recoveries were performed using the transcervical method. All embryo donors (100%) presented estrus regardless of the hormone protocol used. The treatments did not affect the ovarian response, as well as quality and viability of embryos (P > 0.05). Treatments had a small dispersion of estrus, 81.81% (9/11) animals from the Traditional protocol group presented estrus 20 h after CIDR withdrawal and 90.9% (10/11) of the Day 0 protocol group presented estrus 24 h. Regarding embryo production, there was no significant difference between hormonal treatments for the different parameters evaluated (P > 0.05). It can be concluded that the Day 0 protocol was not different from the Traditional protocol in crossbred ewes.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison between Day 0 and traditional protocols for estrus synchronization and multiple ovulation in crossbred hair sheep

Magalhães

Júnior

Guimarães

et al. 2019

Journal of Applied Animal Research

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“…Although more studies are needed to confirm our hypothesis, the number of ovulations is decisive for the embryo yields as well as the luteal dynamic, especially the adequate progesterone concentrations until embryo collection day are beneficial to the uterine environment and embryonic development in donor females (Connell et al, ; Kraisoon et al, ). Brasil et al () observed a similar number of CLs to that obtained in this study using 133 mg of total dose of pFSH in Santa Inês ewes. Sanchez‐Dávila et al () reported similar number of CLs in response to 145 and 215 mg of total dose of pFSH for superovulation in goats whereas lower ovarian response was recorded when 80 mg was used; the same was recorded to produce transferable embryos.…”

Section: Discussionsupporting

confidence: 88%

“…The ovarian superstimulation with pFSH dose might contribute to the variability in superovulatory responses in ewes (Bartlewski, Fuerst, Alexander, & King, 2009 (Connell et al, 2013;Kraisoon et al, 2015). Brasil et al (2016) observed a similar number of CLs to that obtained in this study using 133 mg of total dose of pFSH in Santa Inês ewes. Sanchez-Dávila et al (2014) reported similar number of CLs in response to 145 and 215 mg of total dose of pFSH for superovulation in goats whereas lower ovarian response was recorded when 80 mg was used; the same was recorded to produce transferable embryos.…”

Section: Discussionsupporting

confidence: 88%

Early luteal development in Santa Inês ewes superovulated with reduced doses of porcine follicle‐stimulating hormone

Rodriguez

Maciel

Uscátegui

et al. 2018

Reprod Domestic Animals

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Contents The aim was to compare the early luteal development in ewes superovulated with different doses of pFSH. Twenty‐nine Santa Inês ewes received a progesterone device (CIDR®) for 8 days. Gonadotrophic treatment started on Day 6: G200 (control, n = 9, 200 mg); G133 (n = 10, 133 mg); and G100 (n = 10, 100 mg of pFSH). On Day 6, all females received eCG (300 IU). B‐mode and spectral Doppler ultrasonography were performed daily during the early luteal phase (Days 11–15) to monitor the development of corpora lutea (CLs; dimensions) and ovarian arteries indices. CLs were also classified as normal or prematurely regressed (PRCL) on Day 15 by videolaparoscopy. Ewes from G100 and G133 showed gradual increase in luteal diameter during the early luteal phase (p < 0.001), whereas G200 animals presented increase from Day 11 to Day 13, and then decrease on Days 14 and 15 (p < 0.001). The G200 females showed greater percentage of PRCL (45.20%) than those of the other groups (p < 0.001). The normal CLs number was greater in G100 than in G133 (p = 0.04), while the PRCL number was greater in G200 than in the other groups (p = 0.03). Resistive index (RI) was greater in G200 than in G100 (p = 0.02). RI was lower in Day 12 than Day 15 (p = 0.02). Pulsatility index (PI) was greater on Days 14 and 15 (p < 0.01). In conclusion, the lowest dose of pFSH (100 mg) can be considered sufficient for an efficient superovulatory response in sheep, producing better CLs development dynamic in early luteal phase and ovarian blood perfusion and smaller number of PRCL than the traditional (200 mg) pFSH dose.

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Ovarian response to P4‐PGF‐FSH treatment in Suffolk sheep and P4‐PGF‐PMSG synchronization in cross‐bred ewes, for IVD and ET protocol

Ciornei¹,

Drugociu²,

Ciornei³

et al. 2022

Veterinary Medicine & Sci

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Background:The success of an embryo transfer protocol in sheep depends on many factors, but the choice of drugs for the desired superovulation as well as the conception rate (CR) are most essential. Reproductive activity in sheep is characterized by a seasonality influenced by several factors such as photoperiod, latitude, temperature, nutrition and breed. Reproductive seasonality and nutritional condition are the main factors that influence embryo production in sheep. In sheep, some anatomical peculiarities limit the application of traditional reproductive biotechnologies used in cattle. Objectives:The aim of this study was to conclude on the effectiveness of a wider on farm in vivo embryo transfer development programme in Suffolk sheep by streamlining hormone therapies and optimizing technique.Methods: A total number of 60 sheep and three rams were included in this study, divided into two groups (receptors and donors). Donor Suffolk sheep were treated for superovulation using the P4-PGF-FSH multiple ovulation embryo transfer (MOET) protocol, while the cross-bred recipients' group was synchronized with P4-PGF-PMSG.Results: On the first day after superovulation, all ovaries had more than five dominant follicles, while corpora lutea were later observed in 83.3% sheep. The recovery rate was 83.3%, while 72.9% embryos were transferable. Embryos were transferred directly into recipients. Fertility after 30 days was 68.57%, lambing rate was 91.6% and CR was 62.85%. This study showed that veterinary drugs (P4, FSH, LH, PMSG, PGF) used for superovulation optimized by us were capable of producing by this improved technique the optimization of the reproduction indices at embryo-transfer (ET) and to be able to be used successfully. Conclusions:The application of an MOET protocol has a positive effect in the production of in vivo embryo production (IVD) embryos in Suffolk sheep and can guarantee the success of embryo transfer activity to ewes with lower genetic merit. Our research aimed at representing a model for sheep farms for a rapid improvement of productive traits.

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Superovulatory and embryo yielding in sheep using increased exposure time to progesterone associated with a GnRH agonist

Cited by 19 publications

References 20 publications

Comparison between Day 0 and traditional protocols for estrus synchronization and multiple ovulation in crossbred hair sheep

Comparison between Day 0 and traditional protocols for estrus synchronization and multiple ovulation in crossbred hair sheep

Early luteal development in Santa Inês ewes superovulated with reduced doses of porcine follicle‐stimulating hormone

Ovarian response to P4‐PGF‐FSH treatment in Suffolk sheep and P4‐PGF‐PMSG synchronization in cross‐bred ewes, for IVD and ET protocol

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