Superovulatory response of river bufalo ( )

Karaivanov, C.; Kacheva, D.; Petrov, M.; Vlahov, K.; Sapundjiev, E.

doi:10.1016/0093-691x(90)90503-l

Cited by 15 publications

(4 citation statements)

References 16 publications

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“…However, low efficiency (Misra, 1993;Karaivanov et al, 1990;Presicce et al, 2002), manifesting relatively low yield of in vivo-derived embryos, shifted interest to in vitro embryo production technology as a potential alternative method for faster propagation of superior germplasm.…”

Section: Introductionmentioning

confidence: 98%

Full-term delivery of river buffalo calves (2n=50) from in vitro-derived vitrified embryos by swamp buffalo recipients (2n=48)

et al. 2007

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Section: Introductionmentioning

confidence: 98%

Full-term delivery of river buffalo calves (2n=50) from in vitro-derived vitrified embryos by swamp buffalo recipients (2n=48)

et al. 2007

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“…Recent advances in embryo transfer technology have allowed progress towards increasing the number of offspring produced from genetically superior buffaloes. The application of bovine embryo transfer technology to buffalo has had only a limited success (Vlahov et al, 1985;Kurup, 1988;Karaivanov et al, 1990; Madan, 1990). However, there is slow, yet steady, progress in terms of superovulatory response and embryo recovery (Mishra et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis)

Totey¹,

Singh²,

Taneja³

et al. 1992

Reproduction

124

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Cumulus-oocyte complexes, 5596, were cultured for 24 h in either TCM-199 or Ham's F-10 with or without gonadotrophins and supplemented with either 20% buffalo oestrous serum (BES) or fetal calf serum (FCS). The maturation rates of oocytes cultured in TCM-199 or Ham's F-10 medium supplemented with 20% BES were 47.4 +/- 17.8 and 44.8 +/- 25.6, respectively. Addition of luteinizing hormone (LH) (5 micrograms ml-1) significantly improved the maturation rate in the Ham's F-10 medium supplemented with 20% BES (76.8 +/- 18.3), but follicle-stimulating hormone (FSH) (0.5 micrograms ml-1) and oestradiol (1 microgram ml-1) failed to synergize with LH (71.7 +/- 19.5). In the TCM-199 system, LH failed to enhance the maturation rate but the addition of FSH and oestradiol significantly enhanced the proportion of mature oocytes (42.7 +/- 1.4 and 81.7 +/- 14.5, respectively; P less than 0.05). Frozen-thawed spermatozoa prepared in Bracket and Oliphant (BO) medium and treated with 5 mmol caffeine 1(-1) + 10 micrograms heparin showed a higher fertilization rate (29.8%) than those treated in Hepes-Talp and treated with 10 micrograms heparin ml-1 (19.6%). Fertilization rate was significantly improved when fresh ejaculated spermatozoa treated with 5 mmol caffeine 1-1 and 10 micrograms heparin in BO medium (50%) was used. Rate of cleavage and development were also higher when in vitro fertilization was carried out with fresh ejaculated spermatozoa treated with caffeine and heparin (34.1 and 36.8%, respectively) than with frozen-thawed spermatozoa (27.0 and 22.0%, respectively). Development rate was enhanced when fertilized ova were cultured in ligated rabbit oviduct (28.0%) than when co-cultured on oviductal cell monolayers (8.2%). The results indicate that oocytes cultured in medium supplemented with BES and gonadotrophins reveal high rates of maturation and development to the blastocyst stage after fertilization with fresh ejaculated spermatozoa.

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“…Group I resulted in to recovery of 6 total embryos and 1 unfertilized ova with a mean of 0.86 ± 0.26 and 0.14 ± 0.14, respectively, per collection with overall mean percentage of embryo recovery rate of 35.71 ± 9.91, In Group II resulted in to recovery of 11 total embryos and 1 unfertilized ova with a mean of 1.57 ± 0.48 and 0.14 ± 0.14, respectively, per collection with overall mean percentage of embryo recovery rate of 47.14 ± 10.30 (Table 1). The percentage of total recovery of embryos and ova is higher than the observations of (Karaivanov et al 1990) [10] in Russian cows and of (Nilchuen et al 2012) [18] in Kamphaeng Sean beef cattle. The lesser yield of good…”

Section: Resultsmentioning

confidence: 51%

Effect of FSH dose on the super ovulatory response in Punganur Cows

et al. 2024

Int. J. Adv. Biochem. Res.

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The study was aimed to evaluate the super ovulatory response in Punganur cows by using low doses of FSH wherein donor cows were treated with 160 and 200 mg of Folltropin ® V to assess the ovarian response, follicular development, total number of ovulations per animal, embryos recovered, and concentrations of estrogen at different stages of the treatment. The results indicated that 200 mg FSHp has significantly higher ovarian response with a greater number of follicles per animal, but with no significant difference in total number of ovulations, embryos recovered, and viable embryos between groups. The concentration of estrogen was significant among different treatment days within each group, but with no significant difference between groups. However, a negative correlation was observed between the concentration of estrogen on the day of initiation of superovulation with number of ovulations and embryos collected indicating an inverse relationship. In conclusion, super ovulatory response, follicular growth, and embryo recovery rate was superior with 200 mg compared to 160 mg FSH.

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Superovulatory response of river bufalo ( )

Cited by 15 publications

References 16 publications

Full-term delivery of river buffalo calves (2n=50) from in vitro-derived vitrified embryos by swamp buffalo recipients (2n=48)

Full-term delivery of river buffalo calves (2n=50) from in vitro-derived vitrified embryos by swamp buffalo recipients (2n=48)

In vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis)

Effect of FSH dose on the super ovulatory response in Punganur Cows

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