Superoxide anion monitoring in epileptic brains with a near-infrared fluorescent probe

Ying, Weiwu; Dong, Fuxing; Shi, Yifan; Zhan, Ziyi; Wang, Shuwan; Lv, Li; Liu, Huizhen; Liu, Ling; Zheng, Yun‐Wen; Zhang, Ling

doi:10.1016/j.dyepig.2023.111155

Cited by 9 publications

(3 citation statements)

References 54 publications

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“…Mice were injected intraperitoneally with saline (0.2 mL) every other day in the control group. While in the epileptic group, PTZ was administered intraperitoneally to mice every other day. , In the treatment group, epileptic mice were intraperitoneally injected using N -acetylcysteine (NAC) (20 mg/kg) once a day for 5 days.…”

Section: Methodsmentioning

confidence: 99%

“…Following each administration, the behavior and seizure activity of the mice were continuously monitored for 60 min. The seizure activity of the mice was classified and scored on a modified Racine Scale. , The PTZ ignition epilepsy model was successfully induced when five consecutive seizure scores reached a threshold of ≥2. The behavior of the mice was monitored with a video camera throughout the experiment.…”

Section: Methodsmentioning

confidence: 99%

“…The design and synthesis of the probe directly affect the final imaging effect. To date, some fluorescent probes have been reported for the detection of O 2 •– in epilepsy. , Few chemiluminescent probes have been in detecting O 2 •– in vivo (Table S1). − However, no probe has been reported that can simultaneously switch on dual optical modes (FL and CL) in response to the O 2 •– in epilepsy.…”

Section: Introductionmentioning

confidence: 99%

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Activatable Dual-Optical Molecular Probe for Bioimaging Superoxide Anion in Epilepsy

Si,

Lv,

Shi

et al. 2024

Anal. Chem.

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Superoxide anion (O 2•− ) plays a pivotal role in the generation of other reactive oxygen species within the body and is closely linked to epilepsy. Despite this connection, achieving precise imaging of O 2•− during epilepsy pathology remains a formidable challenge. Herein, we develop an activatable molecular probe, CL-SA, to track the fluctuation of the level of O 2•− in epilepsy through simultaneous fluorescence imaging and chemiluminescence sensing. The developed probe CL-SA demonstrated its efficacy in imaging of O 2•− in neuronal cells, showcasing its dual optical imaging capability for O 2•− in vitro. Furthermore, CL-SA was successfully used to observe aberrantly expressed O 2•− in a mouse model of epilepsy. Overall, CL-SA provides us with a valuable tool for chemical and biomedical studies of O 2•− , promoting the investigation of O 2 •− fluctuations in epilepsy, as well as providing a reliable means to explore the diagnosis and therapy of epilepsy.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%