Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state

Melchiorre, Mariana; Robert, Germán; Trippi, Victorio S.; Racca, R. W.; Lascano, Ramiro

doi:10.1007/s10725-008-9322-3

Cited by 65 publications

(27 citation statements)

References 55 publications

(54 reference statements)

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“…Significantly higher GR activity under PEG stress in WP as compared to other treatments would lead to a greater availability of GSH and thus better ROS detoxification. This is also supported by a previous study which demonstrated that overexpression of GR in wheat protoplast led to an increase in GSH content and GSH/GSSG ratio, which resulted in the maintenance of cellular redox state under drought stress (Melchiorre et al 2009). A better ROS detoxification was also obvious from the decrease in MDA content in WP treatment as compared to other treatments, particularly under PEG stress (Fig.…”

Section: Discussionsupporting

confidence: 84%

Comparative evaluation of hydro-, chemo- , and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard (Brassica juncea L.)

et al. 2010

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The present study was aimed to evaluate the effect of different seed priming methods to enhance the sodium chloride (NaCl) and polyethylene glycol-8000 (PEG-8000) stress tolerance in Indian mustard (Brassica juncea L.). Seeds subjected to different priming treatments such as water (hydro-priming), calcium chloride (CaCl 2 ) (chemo-priming), and abscisic acid (ABA) (hormonalpriming) showed increased rate of germination as compared to non-primed seeds. The primed and non-primed seeds were grown for 15 days and then the seedlings were independently subjected to iso-osmotic salt (150 mM NaCl) or PEG-8000 (20%) stress. The different biochemical responses were studied 10 days after treatment. Under NaCl and PEG stress, the dry weight and total chlorophyll content were higher in primed sets as compared to nonprimed treatment which was also evident by the phenotype of the seedlings. In general, the higher activities of superoxide dismutase and glutathione reductase resulted in lower oxidative damage, in terms of malondialdehyde content, under NaCl and PEG stress in hydro-primed set as compared to non-primed, ABA-, and CaCl 2 -primed treatments. Besides, the level of total phenolics and accumulation of osmolytes such as free proline, glycine betaine, and total soluble sugars was also lower in hydro-primed set as compared to other primed and non-primed treatments. The study thus suggests the use of hydro-priming as a simple and cost-effective strategy to alleviate the NaCl and PEG induced stress in B. juncea.

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Section: Discussionsupporting

confidence: 84%

Comparative evaluation of hydro-, chemo- , and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard (Brassica juncea L.)

et al. 2010

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“…These radicals are very reactive and would enter numerous further reactions and thereby generate various types of ROS, which would finally destroy the entire photosynthetic apparatus. The first defense line against ROS is dismutation of two superoxide molecules to hydrogen peroxide (H 2 O 2 ) and oxygen by superoxide dismutase (SOD) (Melchiorre et al, 2009). …”

Section: Foliar Application Of Tio 2 Np Under Water-deficit Stressmentioning

confidence: 99%

Effects of TiO2 nanoparticles and water-deficit stress on morpho-physiological characteristics of dragonhead (Dracocephalum moldavica L.) plants

Mohammadi

Esmailpour

Gheranpaye

2016

AAS

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Water-deficit stress is the most important environmental factors limiting plant growth, and production. Nano-titanium dioxide (nano anataseTiO 2 ) can have various profound effects on the crop physiological, biochemical and morphological characteristics. In the present research, the influences of different concentrations ofTiO 2 nanoparticles (NPs) (0, 10 and 40 ppm) and water-deficit stress on Dragonhead (Dracocephalum moldavica L.) were investigated in a factorial experiment based on randomized complete block design with three replications. Results showed that under normal irrigation, foliar application of 10 ppm TiO 2 NPs increased plant shoot dry mass and essential oils content. Under water-deficit stress condition, plants treated with 10 ppm TiO 2 NPs had more proline and much less H 2 O 2 and malondialdehyde content as compared to untreated plants. Therefore, it can be concluded that proper concentration of TiO 2 NPs probably can be used as an exogenous stimuli for improvement of shoot growth and essential oil content in plants. Furthermore, water-deficit stress-induced damages such as oxidative stress and membrane damage can be ameliorated by foliar application of TiO 2 NPs at appropriate concentrations. Sušni stres je eden izmed najpomembnejših okoljskih dejavnikov, ki omejujejo rast in produktivnost rastlin. Uporaba nano delcev titanovega dioksida (TiO 2 nano anataza) ima lahko velike učinke na fiziološke, biokemične in morfološke lastnosti gojenih rastlin. V tej raziskavi je bil v naključnem faktorskem bločnem poskusu preučevan vpliv različnih koncentracij nano delcev TiO 2 (0, 10 in 40 ppm) in sušnega stresa na moldavsko kačjeglavko (Dracocephalum moldavica L.) v treh ponovitvah. Rezultati so pokazali, da sta se suha masa in vsebnost eteričnih olj povečali pri normalnem namakanju in listnem dodajanju 10 ppm TiO 2 nano delcev. V razmerah sušnega stresa so vsebovale rastline, ki so bile tretirane z 10 ppm TiO 2 več prolina in mnogo manj H 2 O 2 in malonildialdehida v primerjavi z netretiranimi rastlinami. Na osnovi tega bi lahko zaključili, da bi z uporabo primerne koncentracije nano delcev TiO 2 kot zunanjega stimulanta, lahko izboljšali rast poganjkov in vsebnost eteričnih olj pri tej rastlini. Poškodbe kot sta oksidativni stres in poškodovanost membran, ki nastanejo zaradi sušnega stresa, bi lahko ublažili s foliarno aplikacijo primerne koncentracije nanodelcev TiO 2 .

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“…Overexpression of WRKY25 enhanced the expression level of heat-responsive genes HsfA2, HsfB1, HsfB2a, and HSP101 ). Also, expression pattern of glutathione reductases was studied in rice, wheat, barley, and maize during photooxidative stress using northern blot analysis (Melchiorre et al 2009). and Table 3.2 Expression studies of plant microRNAs during abiotic stresses using different transcriptomic approaches…”

Section: Northern Blot Hybridizationmentioning

confidence: 99%

Transcriptomics of Heat Stress in Plants

Sailaja

Mangrauthia

Neelamraju

et al. 2014

Improvement of Crops in the Era of Climatic Changes

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High-temperature stress is a major abiotic stress that affects various biological processes of plants such as biochemical and physiological response, growth, development, and yield. High-temperature stress has critical effects at cellular and molecular levels also. The increased concentration of regulatory proteins such as heat shock transcription factors (Hsfs) is a major molecular response that occurs during heat stress. These regulatory proteins in turn regulate the expression of heat shock protein (HSP) genes that act as critical players during stress to maintain cell homeostasis. Besides HSPs, the other metabolic and regulatory genes, signaling compounds, compatible osmolytes, and antioxidants too play an important role during heat stress in plants. Apart from the protein-coding genes, recent studies have shown that noncoding microRNAs (miRNAs) also play a key role during heat stress by modulating the gene expression at the transcription and post-transcriptional level. The transcriptome approaches are important to understand the molecular and cellular changes occurring in response to heat stress. The approaches rely mostly by adopting the traditional methods like Northern blot/RNA blot and reverse transcription PCR (RT-PCR), where the expression of the genes can be studied in different tissues and cells, whereas the extent of their expression can be achieved by quantitative PCR or real time PCR. Further, the genome-wide expression profiling tools such as microarray analysis, next-generation sequencing, and RNA sequencing offer a great potential in this direction. This chapter primarily provides the current understanding on the role of regulatory genes (transcription factors), HSP genes, metabolic genes, signaling compounds, osmolytes, reactive oxygen species, 50 B. Sailaja et al.and miRNAs as well as other small RNAs of plants under high temperature. In addition, it gives a brief account of various transcriptome approaches to study the expression profiling of genes during heat stress.

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Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state

Cited by 65 publications

References 55 publications

Comparative evaluation of hydro-, chemo- , and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard (Brassica juncea L.)

Comparative evaluation of hydro-, chemo- , and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard (Brassica juncea L.)

Effects of TiO2 nanoparticles and water-deficit stress on morpho-physiological characteristics of dragonhead (Dracocephalum moldavica L.) plants

Transcriptomics of Heat Stress in Plants

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