Superoxide dismutase decreases early reperfusion release of conjugated dienes following regional canine ischemia

Lesnefsky, Edward J.; Fennessey, P. M.; Benthuysen, K. M. Van; McMurtry, Ivan F.; Travis, Victoria L.; Horwitz, Lawrence D.

doi:10.1007/bf01907928

Cited by 39 publications

(7 citation statements)

References 21 publications

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“…In a similar fashion, the detection of conjugated dienes during early reperfusion based upon their signature absorbance also has limited specificity. 74 Individual oxidized linoleic acid products, including the signature compounds of hydroxy-and peroxy-octadecadienoic acids, have been detected after regional myocardial ischemia and reperfusion, with identification by gas chromatography methods, increasing analytical precision, and specificity. 75,76 These compounds, although suspicious for a CL origin, are, of course, not definitively linked to CL.…”

Section: Oxidative Damagementioning

confidence: 99%

Cardiolipin Remodeling in the Heart

Sparagna¹,

Lesnefsky²

2009

Journal of Cardiovascular Pharmacology

119

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Cardiolipin (CL) is a mitochondrial phospholipid that fundamentally contributes to the function of many proteins in the inner mitochondrial membrane, where it is actively involved in the integrity and flux of the electron transport chain. In the heart, functional CL is linoleic acid rich, and the loss of linoleic acid content is associated with cardiac disorders including ischemia and reperfusion, heart failure, and diabetes, as well as the X-linked recessive disease, Barth syndrome. To attain its high levels of linoleic acid, newly synthesized CL must initially undergo remodeling. CL modification and depletion by pathological processes may represent a failure of this remodeling pathway or activation of an alternative "pathological remodeling" pathway that causes the substitution of higher molecular weight, polyunsaturated fatty acyl side chains such as arachidonic or docosahexaenoic acid onto CL. This remodeling may occur in response to the alteration of CL by oxidative mechanisms, but the substituted side chains can also provoke further oxidation of CL. It is increasingly thought that cardiac pathology may result, at least partially, due to changes in CL resulting from the pathological remodeling process. Dietary interventions may restore CL to its linoleic acid-rich form and improve cardiac function by redirecting the remodeling process.

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Section: Oxidative Damagementioning

confidence: 99%

Cardiolipin Remodeling in the Heart

Sparagna¹,

Lesnefsky²

2009

Journal of Cardiovascular Pharmacology

119

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“…As a marker of oxidant-mediated lipid peroxidation, conjugated diene (CD) levels were determined spectrophotometrically in CS plasma after chloroform-methanol 2:1 (vol/vol) extraction as previously described. 7 CD concentration was expressed as absorbance (A) at a wavelength of 240 nm per 0.5 mL plasma.…”

Section: Cs Blood Analysismentioning

confidence: 99%

Reduction of systolic and diastolic dysfunction by retrograde coronary sinus perfusion during off-pump coronary surgery

Castellà

2004

The Journal of Thoracic and Cardiovascular Surgery

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“…The thermodilution probe was placed in the main pulmonary artery and connected to a cardiac output computer (Model 9520A, American Edwards Laboratory, Santa Ana, Calif. Conjugated diene levels (a marker of lipid peroxidation) were determined from samples of coronary sinus blood as described by Lefnesky and coworkers. 15 Antioxidant reserve capacity was measured by the method of Godin and coworkers 16 to determine vulnerability of hypoxemic and reoxygenation myocardium to subsequent oxidant stress. Myocardial homogenates were exposed to 0 to 5 mmol/L t-butylhydroperoxide for 30 minutes at 37 ° C with subsequent measure of thiobarbituric acid-reactive substance.…”

Section: Methodsmentioning

confidence: 99%

Studies of hypoxemic/reoxygenation injury: Without aortic clamping

Ihnken

Morita²,

Sherman³

et al. 1995

The Journal of Thoracic and Cardiovascular Surgery

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This study tests the hypothesis that antioxidants administered before reoxygenation can reduce oxygen-mediated damage and improve myocardial performance. Of 25 Duroc-Yorkshire piglets (2 to 3 weeks, 3 to 5 kg) five underwent 60 minutes of cardiopulmonary bypass without hypoxemia (control group), and five others underwent 30 minutes of hypoxemia on cardiopulmonary bypass with a circuit primed with oxygen tension about 25 mm Hg blood followed by reoxygenation on cardiopulmonary bypass (no treatment). In vitro studies were performed to obtain the optimal dosage of the antioxidants N-(2-mercaptopropionyl)-glycine and and catalase to be used in subsequent in vivo experimental studies; cardiac homogenates were incubated in 0 to 5 mmol/L concentrations of the oxidant t-butylhydroperoxide and malondialdehyde production was measured. Fifteen piglets were made hypoxemic on cardiopulmonary bypass for 30 minutes, and the antioxidants N-(2-mercaptopropionyl)-glycine at either 30 or 80 mg/kg body weight or N-(2-mercaptopropionyl)-glycine, 30 mg/kg body weight, and catalase, 50,000 U/kg body weight, were added to the cardiopulmonary bypass circuit 15 minutes before reoxygenation. Left ventricular contractility, which was expressed as end-systolic elastance, was measured by conductance catheter before hypoxemia and after reoxygenation. Myocardial antioxidant reserve capacity was determined after reoxygenation by incubating cardiac homogenates in the oxidant t-butylhydroperoxide and measuring subsequent malondialdehyde elution. The in vitro bioassay studies showed a dose-dependent reduction of lipid peroxidation with N-(2-mercaptopropionyl)-glycine, with maximal benefits of a 40% decrease and malondialdehyde elaboration occurring with N-(2-mercaptopropionyl)-glycine and catalase compared with untreated cardiac homogenates. Cardiopulmonary bypass (no hypoxemia) caused no oxidant damage or changes in contractile function after cardiopulmonary bypass. Reoxygenation without treatment raised conjugated diene levels 57%,* lowered antioxidant reserve capacity 51%,* and was associated with only 38%* recovery of contractile function (p < 0.05 vs control). In contrast, treatment with antioxidants avoided lipid peroxidation, maintained antioxidant reserve capacity, and resulted in a dose-dependent improvement in left ventricular contractility with complete recovery occurring in N-(2-mercaptopropionyl)-glycine and catalase-treated piglets (*p < 0.05 vs no treatment). This study confirms the occurrence of hypoxemic/reoxygenation injury in immature hearts placed on cardiopulmonary bypass and shows that biochemical and functional damage can be counteracted by adding antioxidants to the cardiopulmonary bypass priming fluid. Contractile function improved in a dose-dependent manner, and oxygen-mediated damage could be avoided by mercaptopropionyl glycine/catalase treatment.(ABSTRACT TRUNCATED AT 400 WORDS)

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Superoxide dismutase decreases early reperfusion release of conjugated dienes following regional canine ischemia

Cited by 39 publications

References 21 publications

Cardiolipin Remodeling in the Heart

Cardiolipin Remodeling in the Heart

Reduction of systolic and diastolic dysfunction by retrograde coronary sinus perfusion during off-pump coronary surgery

Studies of hypoxemic/reoxygenation injury: Without aortic clamping

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