Superoxide dismutase in normal cycling human ovaries: immunohistochemical localization and characterization

Suzuki, Takashi; Sugino, Norihiro; Fukaya, Takao; Sugiyama, Shunsuke; Uda, Takaaki; Takaya, Risa; Yajima, Akira; Sasano, Hironobu

doi:10.1016/s0015-0282(99)00332-5

Cited by 106 publications

(84 citation statements)

References 25 publications

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“…Endometrial samples were histologically diagnosed as late proliferative phase according to the criteria of Noyes et al [32]. Tissue samples were washed with Phenol Red-free DMEM containing 4 mM glutamine, 50 µg/ml streptomycin and 50 U/ml penicillin, and minced into small pieces of <1 mm 3 . ESC were isolated as reported previously [33].…”

Section: Esc Isolationmentioning

confidence: 99%

“…50: [215][216][217][218][219][220][221][222][223][224][225] 2004) eactive oxygen species (ROS), including superoxide radicals, cause cell damage, whereas superoxide dismutase (SOD) is an enzyme specific to scavenging superoxide radicals and protects cells from oxygen radical cytotoxicity. Oxidative stress and its defense system have been reported to play important roles in the regulation of reproductive function [1][2][3][4][5][6][7]. In the human endometrium, SOD activities decrease and ROS levels increase in the late secretory phase, just before menstruation, suggesting that these changes in SOD and ROS may be involved in endometrial breakdown [8].…”

mentioning

confidence: 99%

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Withdrawal of Ovarian Steroids Stimulates Prostaglandin F2.ALPHA. Production Through Nuclear Factor-.KAPPA.B Activation via Oxygen Radicals in Human Endometrial Stromal Cells: Potential Relevance to Menstruation

et al. 2004

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Abstract. The present study was undertaken to investigate whether withdrawal of estrogen and progesterone (EP-withdrawal) stimulates prostaglandin F2α (PGF2α) production through oxygen radical (ROS)-induced NF-κB activation in human endometrial stromal cells (ESC). To study the EPwithdrawal, ESC that had been treated with estradiol (E, 10 -8 M) and medroxyprogesterone acetate (MPA, 10 -6 M) for 12 days were then incubated with or without E+MPA for a further 11 days. PGF2α concentrations in the medium and cyclooxygenase-2 (COX-2) mRNA levels were significantly increased after EP-withdrawal, while they were unchanged by the continuous treatment with E+MPA. When ESC were incubated with N-acetyl-L-cysteine (Nac, 50 mM), an antioxidant, during EP-withdrawal, Nac blocked the increases in PGF2α production and COX-2 mRNA expression caused by EPwithdrawal. Next, we examined whether ROS generated in response to EP-withdrawal acted through NF-κB activation. Electrophoretic mobility shift assay revealed that EP-withdrawal caused marked increases in NF-κB DNA binding activity, which was completely suppressed by Nac. Furthermore, when ESC were incubated with MG132 (3 µM), which inhibits NF-κB activation, during EPwithdrawal, MG132 blocked the increases in PGF2α production and COX-2 mRNA expression caused by EP-withdrawal. In conclusion, EP-withdrawal stimulates COX-2 expression and PGF2α production through ROS-induced NF-κB activation, suggesting a possible mechanism for menstruation. Key words: Endometrial stromal cell, Prostaglandin F2α, NF-κB, Superoxide dismutase, Superoxide radical (J. Reprod. Dev. 50: [215][216][217][218][219][220][221][222][223][224][225] 2004) eactive oxygen species (ROS), including superoxide radicals, cause cell damage, whereas superoxide dismutase (SOD) is an enzyme specific to scavenging superoxide radicals and protects cells from oxygen radical cytotoxicity. Oxidative stress and its defense system have been reported to play important roles in the regulation of reproductive function [1][2][3][4][5][6][7]. In the human endometrium, SOD activities decrease and ROS levels increase in the late secretory phase, just before menstruation, suggesting that these changes in SOD and ROS may be involved in endometrial breakdown [8]. Recently, much attention has been focused on the reports that ROS act as second messengers in the regulation of cellular function [9-

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Section: Esc Isolationmentioning

confidence: 99%

mentioning

confidence: 99%

Withdrawal of Ovarian Steroids Stimulates Prostaglandin F2.ALPHA. Production Through Nuclear Factor-.KAPPA.B Activation via Oxygen Radicals in Human Endometrial Stromal Cells: Potential Relevance to Menstruation

et al. 2004

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“…SOD and CAT) (Suzuki et al, 1999;Mouatassim et al, 1999). SOD activity and CAT activity have been demonstrated in human FFls (Carbone et al, 2003;Pasqualotto et al 2009;Bausenwein et al, 2010).…”

Section: Relationship Between Activity Of Antioxidant Enzymes Sod Andmentioning

confidence: 98%

Ovarian Biomarkers in Infertility

Vangelov¹,

Dineva²,

Todorova³

et al. 2012

Trends in Immunolabelled and Related Techniques

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“…These biomarkers play roles in development of follicles and regulate processes involved in oocyte maturation, steroidogenesis and folliculogenesis (Suzuki et al, 1999). Studies by Shiotani et al (1991) on immunohistochemistry of antral follicles revealed the presence of antibody to Ad4-binding protein, which is a steroidogenic transcription factor that causes transcription of enzymes involved in steroidogenesis.…”

Section: Issn: 2320-5407mentioning

confidence: 99%