Superoxide dismutase induces differentiation of friend erythroleukemia cells

Beckman, Barbara S.; Balin, Arthur K.; Allen, Robert G.

doi:10.1002/jcp.1041390220

Cited by 68 publications

(36 citation statements)

References 39 publications

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“…Overexpression of a the Mn-SOD gene stimulates changes in the state differentiation in human melanoma (59) and mouse C10HT1/2 cells (60). Interestingly, other antioxidants fail to stimulate differentiation, but some oxidants do (57,58). Shifts in the redox environment resulting from the production of In contrast to previous reports of age-associated increases in generation of both ⅐ O 2 Ϫ and H 2 O 2 (9, 14), we observed no agingassociated increase in oxidation.…”

Section: Discussioncontrasting

confidence: 99%

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Development and Age-associated Differences in Electron Transport Potential and Consequences for Oxidant Generation

Allen

Keogh²,

Tresini

et al. 1997

Journal of Biological Chemistry

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We determined the activities of NADH dehydrogenase (ND), succinate dehydrogenase, and cytochrome c oxidase (COX) in 29 skin fibroblast lines established from donors ranging in age from 12 gestational weeks to 94 years. The results of this study demonstrate that all three of the enzyme activities examined are greater in adult-derived fibroblasts than in the fetal cell lines. The ratio of enzyme activities that control electron entry into and exit from the electron transport chain varied directly with lucigenin-detected chemiluminescence (an indicator of ⅐ O 2 ؊ generation) and inversely with H 2 O 2 generation. These results indicate a clear difference in the predominant oxidant species generated during fetal and adult stages of life. We also examined the mRNA abundances of different components of the electron transport chain complexes. We observed higher abundances of mitochondrial encoded mRNAs (COX 1 and ND 4) in cell lines established from adults than in fetal cells. No differences in the mRNA abundances of the nuclear encoded sequences (COX 4 and ND 51) were observed in fetal and postnatal-derived lines. Succinate dehydrogenase mRNA abundance was greater in cell lines established from postnatal donors than in fetal cell lines. No significant differences between cell lines established from young and old adults were detected in any of the parameters examined.Mitochondria are the primary site of aerobic energy production and are thus the major site of generation of reactive oxygen species (ROS), 1 such as oxygen-centered free radicals and peroxides, which are by-products of metabolism (1-4). Estimates of the rate of ROS generation can differ greatly; however, it has been demonstrated repeatedly that the relative rate of oxidant generation increases with age and that this increase correlates strongly with age-associated changes in the cellular redox state (5-8). For example, the rates of superoxide ( ⅐ O 2 Ϫ ) (8 -14) and H 2 O 2 generation (7, 10, 14 -17) increase in the cells of aging organisms, whereas the glutathione concentration declines progressively with advancing age (5,6,18,19). Differences in ROS metabolism also exist between cells obtained from early developmental stages and from postnatal tissues (for review, see Refs. 20 and 21). The activities of enzymic antioxidant defenses tend to be lower in fetal cells than in cells derived from postnatal tissues (22-24); however, the GSH concentration is frequently greater in fetal cells than in cells from adults (20,24). Whether these observations reflect differences in the steadystate level of ROS in fetal and postnatal cells is not presently known (20, 21).The rate of mitochondrial ROS generation in cells is largely dependent on the amounts of autoxidizable respiratory carriers and the redox state of electron carriers (1,3,25,26). All electron carriers located prior to one of low abundance will exhibit a greater propensity to be chemically reduced because of the slowing of electron flux (electron stacking; 1, 16, 25, 27). Thus, either age or development-a...

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Section: Discussioncontrasting

confidence: 99%

“…Augmentation of SOD activity via liposomes stimulates differentiation in a nondifferentiating strain of Physarum polycephalum (57) and in Friend cell leukemia (58). Overexpression of a the Mn-SOD gene stimulates changes in the state differentiation in human melanoma (59) and mouse C10HT1/2 cells (60).…”

Section: Discussionmentioning

confidence: 99%

Development and Age-associated Differences in Electron Transport Potential and Consequences for Oxidant Generation

Allen

Keogh²,

Tresini

et al. 1997

Journal of Biological Chemistry

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“…Indeed, increased free radical generation and accumulation of oxidation reaction products have repeatedly been observed during the differentiation and development of a wide variety of cells and organisms (205,243,(271)(272)(273)(274)(275)(276)(277). Itwas our observations inPhysarum (201,205) and Friend cell leukemia (268) that led us to postulate that an upsurge of oxidant production rather than increased antioxidant defenses were stimulatory to pathways involved in differentiation (36,192,201,205,268). Changes in antioxidant defense associated with differentiation may be little more than a response to increasing levels of oxidation.…”

Section: Su Peroxide Dism Utase and Oxidative Stress In Development Amentioning

confidence: 72%

“…The addition of SOD protein to the non-differentiating strain via liposomes was observed to stimulate differentiation (201). Liposomally augmented SOD protein also stimulates differentiation in Friend cell leukemia (268) and overexpression of the gene encoding the manganesecontaining form of the enzyme stimulates differentiation of human melanoma (269) and C10HT1/2 cells (270). Furthermore, overexpression of the SOD-2 gene in fibrosarcoma has been found to limit metastasis (31).…”

Section: Su Peroxide Dism Utase and Oxidative Stress In Development Amentioning

confidence: 99%

“…Alternatively, if the change in SOD activity exceeds any differentiation-associated increase in oxidant generation, antioxidation should stimulate differentiation. In fact, other antioxidants fail to stimulate differentiation, but some oxidants do (201,268). Indeed, increased free radical generation and accumulation of oxidation reaction products have repeatedly been observed during the differentiation and development of a wide variety of cells and organisms (205,243,(271)(272)(273)(274)(275)(276)(277).…”

Section: Su Peroxide Dism Utase and Oxidative Stress In Development Amentioning

confidence: 99%

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Oxidative stress and superoxide dismutase in development, aging and gene regulation

Allen

1998

AGE

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Free radicals and other reactive oxygen species are produced in the metabolic pathways of aerobic cells and affect a number of biological processes. Oxidation reactions have been postulated to play a role in aging, a number of degenerative diseases, differentiation and development as well as serving as subcellular messengers in gene regulatory and signal transduction pathways. The discovery of the activity of superoxide dismutase is a seminal work in free radical biology, because it established that free radicals were generated by ceils and because it made removal of a specific free radical substance possible for the first time, which greatly accelerated research in this area. In this review, the role of reactive oxygen in aging, amyotrophic lateral sclerosis (a neurodegenerative disease), development, differentiation, and signal transduction are discussed. Emphasis is also given to the role of superoxide dismutases in these phenomena.

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Expression of hydrogen peroxide and glutathione metabolizing enzymes in human skin fibroblasts derived from donors of different ages

et al. 1996

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We have examined the activities and mRNA abundance of two hydrogen peroxide metabolizing enzymes (glutathione peroxidase and catalase), glutathione concentration, and the activities of several enzymes that influence glutathione concentration, including glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G-6-PD), and gamma-glutamylcysteine synthetase (gamma-GCS), in 29 skin fibroblast lines derived from donors ranging in age from 14 gestational weeks to 94 years of age. H2O2 metabolizing enzyme activities and mRNA abundances were greater in skin fibroblast cultures established from postnatal donors than in fetally derived cultures. There were no significant differences in either of these parameters in cell lines established from postnatal donors of different ages. Total glutathione concentration decreased with age, but GR activity appeared to be unaffected by age. In order to estimate the ability of the cultures to produce NADPH (an important component of cellular redox status and a cofactor for GR), we determined glucose-6-phosphate dehydrogenase activity and mRNA abundance. We were unable to directly measure gamma-GCS activity or mRNA abundance in any of the skin lines or in fetal lung fibroblast; however, we were able to indirectly demonstrate the presence of this enzyme by stimulating fetal lung fibroblasts with H2O2 following treatment with L-buthionine-S,R-sulfoximine (BSO), an inhibitor of gamma-GCS activity. These results show that some, but not all, age-associated differences in antioxidant defense levels are maintained in a culture environment and are consistent with the hypothesis that developmental stages of life are associated with lower antioxidant defense levels than are present in postnatal phases of life.

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Superoxide dismutase induces differentiation of friend erythroleukemia cells

Cited by 68 publications

References 39 publications

Development and Age-associated Differences in Electron Transport Potential and Consequences for Oxidant Generation

Development and Age-associated Differences in Electron Transport Potential and Consequences for Oxidant Generation

Oxidative stress and superoxide dismutase in development, aging and gene regulation

Expression of hydrogen peroxide and glutathione metabolizing enzymes in human skin fibroblasts derived from donors of different ages

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