Superoxide dismutase isoenzymes of the synovial fluid in rheumatoid arthritis and in reactive arthritides.

Abstract

and the 3Department of Rheumatology, Sahlgren's Hospital, S-413 45 Gothenburg, Sweden SUMMARY The activity of superoxide dismutase isoenzymes was determined in knee joint synovial fluid from 21 patients with rheumatoid arthritis, nine patients with reactive arthritides, and from 17 patients before arthroscopy or arthrotomy for suspected meniscal or ligament injury (controls). Extracellular superoxide dismutase was the major isoenzyme and accounted for about 80% of the total superoxide dismutase activity in the controls. The pattern of the superoxide dismutase isoenzymes was significantly different in rheumatoid arthritis, extracellular (EC) superoxide dismutase being half, CuZn superoxide dismutase double, and the total superoxide dismutase activity a third lower than the activity in the synovial fluid of the controls. The superoxide dismutase activities were similar in synovial fluid from the controls and from the patients with reactive arthritides. The total superoxide dismutase activity was almost three times higher in control synovial fluid than in normal human plasma, but 300 times lower than in human tissues.

Amelioration of antigen-induced arthritis in rats by transfer of extracellular superoxide dismutase and catalase genes

“…In the present study, EC-SOD and catalase were chosen as therapeutic genes. All three types of SOD have been detected in rheumatoid joints, 14,15 but EC-SOD is the only secreted isoenzyme existing in extracellular fluids including synovial fluid and tissue. Furthermore, the relatively long half-life of EC-SOD (20 h) enables it to be efficient in scavenging superoxide radicals.…”

A comparative analysis of Superoxide dismutase activity levels in gingiva and gingival crevicular fluid in patients with chronic Periodontitis and periodontally healthy controls

“…This was achieved by sampling all sites for the same length of time (5 minutes). [13,14] In this study, the results revealed that the activity level of superoxide dismutase was significantly higher in the gingival tissues in the chronic periodontitis group (9.50 ± 2.11 U/ml) as compared to the control group (5.89 ± 0.74 U/ml). This finding seems to confirm several findings in the medical literature about oxidant-antioxidant balance.…”

Chimeric SOD2/3 inhibits at the endothelial-neutrophil interface to limit vascular dysfunction in ischemia-reperfusion

“…Indeed, a reduction in SOD3 has been noted in numerous diseases. Patients with rheumatoid arthritis exhibit a 50% reduction in SOD activity compared with normal controls, suggesting that reduced SOD activity contributes to endothelial dysfunction in patients with this disease (32). Similarly, SOD3 expression is substantially reduced in patients with coronary artery disease (24).…”