Superoxide dismutases—a review of the metal-associated mechanistic variations

Abreu, Isabel A.; Cabelli, Diane E.

doi:10.1016/j.bbapap.2009.11.005

Cited by 459 publications

(464 citation statements)

References 93 publications

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“…Recentemente encontrada no genoma de diversas bactérias (39) e isolada pela primeira vez a partir do citoplasma de bactérias do gênero Streptomyces, a NiSOD possui níquel em seu centro catalítico e mecanismo de catálise não completamente elucidado (42). As estruturas cristalográficas de duas NiSODs, de S. seoulenses e S. coelicolor, foram resolvidas por dois grupos distintos (51,52) que mostraram a organização homohexamérica da enzima além da presença de um motivo de nove aminoácidos (His-Cys-X-X-Pro-Cys-Gly-X-Tyr) na porção catalítica N-terminal.…”

Section: Superóxido Dismutasesunclassified

“…A quantidade de estrelas indica a estrutura quaternária das enzimas, sendo duas para dímeros e quatro para tetrâmeros. Adaptado de (12,42) Apesar de possuírem estruturas e cofatores distintos, todas as classes de superóxido dismutases são capazes de converter duas moléculas de superóxido em oxigênio molecular e peróxido de hidrogênio consumindo dois prótons por meio de reações de oxidação e redução cíclicas que envolvem o átomo metálico (49):…”

Section: Superóxido Dismutasesunclassified

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Identificação dos determinantes estruturais de Fe/MnSODs necessários a especificidade por metal.

Fontolan¹

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Superoxide dismutases (SODs) are metalloenzymes that convert the superoxide anion in molecular oxygen (O2) and hydrogen peroxide (H2O2). The metal in the catalytic center of such enzymes is directly related to their catalysis mechanisms and tridimensional structures. Evolutionarily, FeSOD and MnSOD may have evolved from a common ancestor, because both proteins have homologous primary sequences and superposable crystallographic structures. However, at the catalytic level, both proteins diverged sufficiently to prevent interchange of their metallic centers, which would generate non-functional enzymes, indicating that these proteins have high metal specificity. The objective of this research project is to identify structural determinants of Fe/MnSODs necessary to metal specificity. We intend to use statistical coupling analysis (SCA) to select amino acid residues for site-directed mutagenesis in TrMnSOD e TbFeSODB2. Mutant genes will be constructed and their proteins expressed, purified and crystallized. The tridimensional structure of such mutants will be solved by X-ray crystallography and their enzymatic activities determined, as well as their electron paramagnetic resonance spectra. We hypothesize that SCA is useful to identify amino acid candidates for site-directed mutagenesis to design new SODs with intermediated Fe/Mn specificity, and even metal specificity interconversion, by studying the evolutionary history of these proteins.

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Section: Superóxido Dismutasesunclassified

Identificação dos determinantes estruturais de Fe/MnSODs necessários a especificidade por metal.

Fontolan¹

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“…In mammals, three isoforms of SOD (SOD1, SOD2, SOD3) exist, and all require a redox active transition metal in the active site to accomplish the catalytic breakdown of the superoxide anion. The catalytically active metal can be copper, iron, manganese or nickel [27,28]. Exercise training significantly increased superoxide dismutase activity in the muscle soleus [29].…”

Section: A) Primary Antioxidant Enzymesmentioning

confidence: 99%

“…Manganese (Mn) or copper/zinc (Cu/Zn)-dependent superoxide dismutase (SOD), are located in the matrix and intermembrane space of mitochondria, and quickly dismutate the superoxide generated by mitochondria to H 2 O 2 to prevent oxidative stress [3,27,28,30].…”

Section: A) Primary Antioxidant Enzymesmentioning

confidence: 99%

Antioxidants and Their Importance During Muscular Exercise: A Review

Bjelakovic¹,

Kocić

Radovanović

et al. 2017

FU Med Biol

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“…One of the effects of internal ROS formation at the basis of the eukaryotes was the further development of an extensive array of anti-oxidant measures, ranging from ROS scavenging substances [54] to many different dedicated anti-oxidant enzymatic systems, such as catalase, superoxide dismutases [55] and glutathione peroxidases [56], as well as redox state-regulating enzymes such as thioredoxins [57].…”

Section: A 'Double Whammy': More Information To Protect Against More mentioning

confidence: 99%

What can we infer about the origin of sex in early eukaryotes?

Speijer

2016

Phil. Trans. R. Soc. B

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One contribution of 15 to a theme issue 'Weird sex: the underappreciated diversity of sexual reproduction'. Current analysis shows that the last eukaryotic common ancestor (LECA) was capable of full meiotic sex. The original eukaryotic life cycle can probably be described as clonal, interrupted by episodic sex triggered by external or internal stressors. The cycle could have started in a highly flexible form, with the interruption of either diploid or haploid clonal growth determined by stress signals only. Eukaryotic sex most likely evolved in response to a high mutation rate, arising from the uptake of the endosymbiont, as this ( proto) mitochondrion generated internal reactive oxygen species. This is consistent with the likely development of full meiotic sex from a diverse set of existing archaeal (the host of the endosymbiont) repair and signalling mechanisms. Meiotic sex could thus have been one of the fruits of symbiogenesis at the basis of eukaryotic origins: a product of the merger by which eukaryotic cells arose. Symbiogenesis also explains the large-scale migration of organellar DNA to the nucleus. I also discuss aspects of uniparental mitochondrial inheritance and mitonuclear interactions in the light of the previous analysis.This article is part of the themed issue 'Weird sex: the underappreciated diversity of sexual reproduction'.

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Superoxide dismutases—a review of the metal-associated mechanistic variations

Cited by 459 publications

References 93 publications

Identificação dos determinantes estruturais de Fe/MnSODs necessários a especificidade por metal.

Identificação dos determinantes estruturais de Fe/MnSODs necessários a especificidade por metal.

Antioxidants and Their Importance During Muscular Exercise: A Review

What can we infer about the origin of sex in early eukaryotes?

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