Superoxide generation by human monocytes and macrophages

Weiss, Stephen J.; King, Gerald W.; LoBuglio, Albert F.

doi:10.1002/ajh.2830040102

Cited by 31 publications

(16 citation statements)

References 18 publications

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“…As reported by others (22,(29)(30)(31), SOD or SOD adsorbed to latex particles (but not inactivated SOD) partially inhibited NBT reduction in a concentration-dependent fashion (data not shown). These data indicate the role of superoxide anion (02-) in NBT reduction by intact phagocytes.…”

Section: Contribution Of Different Types Of Leukocytes To Reduction Osupporting

confidence: 61%

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Failure to trigger the oxidative metabolic burst by normal macrophages: possible mechanism for survival of intracellular pathogens.

Wilson¹,

Tsai²,

Remington³

1980

The Journal of Experimental Medicine

299

155

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Reactive oxygen metabolites produced during phagocytosis are important for the microbicidal activity of granulocytes and probably of monocytes as well (1,2). Although the role of reactive oxygen metabolites in the microbicidal activity of macrophages is not established, recent studies (3-6) suggest that they may be important because activated macrophages that exhibit enhanced antimicrobial activity produce increased amounts of these metabolites in response to various stimuli. Because reactive oxygen metabolites are potentially lethal, organisms that survive within phagocytes must either fail to stimulate production of such substances or must be resistant to or fail to encounter them. One such intracellular organism is Toxoplasma gondii. This organism survives and replicates in vitro within human monocyte-derived macrophages and within normal mouse macrophages (7-10). However, other mononuclear phagocytes can kill or inhibit multiplication of Toxoplasma. For example, we recently have observed that Toxoplasma is rapidly destroyed by freshly isolated human blood monocytes (11). In addition, macrophages can be activated to kill or inhibit intracellular replication of Toxoplasma (7-9), and normal macrophages can kill the organism if it is coated with antibody (12, 13). Because the ability of Toxoplasma to survive within mononuclear phagocytes depends upon the stage of differentiation or state of activation of mononuclear phagocytes and also depends upon whether the organism is opsonized, we studied the oxidative metabolic burst by different mononuclear phagocytes during phagocytosis of Toxoplasma. These studies were performed to determine the relationship between survival of this intracellular pathogen and production of toxic oxygen metabolites by these cells. The results revealed that the ability of this organism to survive within certain mononuclear phagocytes is dependent in part upon its being phagocytosed without stimulating production of reactive oxygen metabolites by these cells. Materials a n d M e t h o d sSpecial Reagents. Reagents were obtained from Sigma Chemical Co., St. Louis, Mo., unless

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Section: Contribution Of Different Types Of Leukocytes To Reduction Osupporting

confidence: 61%

“…Thereafter, reaction mixtures were processed and assayed as described by Baehner and Nathan (20), except that the reduced dye was extracted in 3 ml of pyridine. The amount of NBT reduced was calculated with the extinction coefficient E = 2.8 mM -I cm -I (22). lntracellular Location of Reduced NBT.…”

mentioning

confidence: 99%

Failure to trigger the oxidative metabolic burst by normal macrophages: possible mechanism for survival of intracellular pathogens.

Wilson¹,

Tsai²,

Remington³

1980

The Journal of Experimental Medicine

299

155

View full text Add to dashboard Cite

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“…We have shown that through this mechanism, the cells inhibit OH' production, and also myeloperoxidase-dependent production of HOCL. Since these are the most likely contenders for oxidant-mediated tissue damage by neutrophils and other phagocytes (4,28), our results show how red cells could buffer the environment against harmful effects of these cells.…”

Section: Discussionmentioning

confidence: 93%

Human red cells scavenge extracellular hydrogen peroxide and inhibit formation of hypochlorous acid and hydroxyl radical.

Winterbourn¹,

Stern²

1987

J. Clin. Invest.

161

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The ability of intact human red cells to scavenge extracellularly generated H202 and Oj, and to prevent formation of hydroxyl radicals and hypochlorous acid has been examined. Red cells inhibited oxidation of ferrocytochrome c by H202. Cells treated with aminotriazole no longer inhibited, indicating that protection was almost entirely due to intracellular catalase. Contribution by the GSH system was slight, and apparent only with low H202 concentrations when catalase was inhibited by aminotriazole. The cells were about a quarter as efficient at inhibiting cytochrome c oxidation as an equivalent concentration of purified catalase. No inhibition of 0--dependent reduction of ferricytochrome c or nitroblue tetrazolium was observed, although extracted red cell superoxide dismutase inhibited nitroblue tetrazolium reduction at one fortieth the concentration of that in the cells. Red cells efficiently inhibited deoxyribose oxidation by hydroxyl radicals generated from

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“…Human macrophages appear capable of generating substantial amounts of superoxide anion (O 2 * − ) during phagocytosis which may play an important role in bactericidal and other cell functions [44], so macrophages play an essential role not only in the defense against the infection, but are involved in many various pathological processes [45].…”

Section: Ifn-gamma Is Secreted By Thymus-derived (T) Cells Under Certmentioning

confidence: 99%

Effect of Ramadan Fasting On Classically Activated, Oxidative Stress and Inflammation of Macrophage

Lahdimawan¹

2013

iosrphr

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Superoxide generation by human monocytes and macrophages

Cited by 31 publications

References 18 publications

Failure to trigger the oxidative metabolic burst by normal macrophages: possible mechanism for survival of intracellular pathogens.

Failure to trigger the oxidative metabolic burst by normal macrophages: possible mechanism for survival of intracellular pathogens.

Human red cells scavenge extracellular hydrogen peroxide and inhibit formation of hypochlorous acid and hydroxyl radical.

Effect of Ramadan Fasting On Classically Activated, Oxidative Stress and Inflammation of Macrophage

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