Superoxol and amylase inhibition tests for distinguishing gonococcal and nongonococcal cultures growing on selective media

Arko, R J; Odugbemi, T

doi:10.1128/jcm.20.1.1-4.1984

Cited by 13 publications

(4 citation statements)

References 12 publications

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“…Most N. meningitidis strains gave negative reactions, but 98% of group A meningococci were positive (8). The superoxol test appears to lack differential value for B. catarrhalis since both negative and positive reactions have been reported (4,8,177,326).…”

Section: Identification and Biochemical Characteristics Of B Catarrhmentioning

confidence: 96%

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Branhamella catarrhalis: an organism gaining respect as a pathogen.

Catlin

1990

Clinical Microbiology Reviews

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Section: Identification and Biochemical Characteristics Of B Catarrhmentioning

confidence: 96%

“…Weak bubbling or a reaction delayed for 3 s or longer is considered negative. Positive reactions were obtained with 99.9% of N. gonorrhoeae isolates (8,266,326). Most N. meningitidis strains gave negative reactions, but 98% of group A meningococci were positive (8).…”

Section: Identification and Biochemical Characteristics Of B Catarrhmentioning

confidence: 97%

Branhamella catarrhalis: an organism gaining respect as a pathogen.

Catlin

1990

Clinical Microbiology Reviews

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“…on May 5, 2021 by guest http://iai.asm.org/ Downloaded from 6). The metabolic shifts in these obligate aerobes responsible for this are completely unknown.…”

Section: -mentioning

confidence: 99%

“…Despite this in vivo environment of elevated H202 and O2, viable gonococci are routinely isolated from PMN-laden purulent exudates. In comparison with Neisseria meningitidis and the commensal neisserias, all strains of N. gonorrhoeae are reported to contain very high levels of catalase (6,45,47,57). While this may well function as an antiphagocytic or aerotolerance mechanism, it cannot be proposed to replace SOD unless there is no O2 within the cell or unless O2 and its degradation products (other than H202) are not toxic to this organism.…”

mentioning

confidence: 99%

Superoxide dismutase and oxygen toxicity defenses in the genus Neisseria

Archibald¹,

Duong²

1986

Infect Immun

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Among aerotolerant cells, Neisseria gonorrhoeae is very unusual because despite its obligately aerobic lifestyle and frequent isolation from purulent exudates containing polymorphonuclear leukocytes vigorously evolving O2- and H2O2, it contains no superoxide dismutase (SOD). Strains (14) of N. gonorrhoeae were compared with each other and with strains of Neisseria meningitidis, Neisseria mucosa, and Neisseria subflava under identical growth conditions for their contents of the oxy-protective enzymes catalase, peroxidase, and SOD, as well as respiratory chain proteins and activity. The absence of SOD from N. gonorrhoeae strains was demonstrated under a variety of oxygen-stress conditions. The neisserial species showed very different SOD, catalase, and peroxidase profiles. These profiles correlated well with the tolerance of the species to various intra- and extracellular oxygen insults. The high tolerance of N. gonorrhoeae for extracellular O2- and H2O2 appeared to be due to very high constitutive levels of peroxidase and catalase activity combined with a cell envelope impervious to O2-. Nevertheless, N. gonorrhoeae 19424 was much more sensitive to an intracellular flux of O2- than were the other (SOD-containing) neisserial species. The responses of N. gonorrhoeae and N. meningitidis respiratory and oxy-protective enzymes to growth under high and low oxygen tensions were followed, and a novel response, the apparent repression of the respiratory chain intermediates, respiration, and SOD, peroxidase, and catalase activity, was observed. The gonococcal catalase was partially purified and characterized. The results suggest that the very active terminal oxidase, low pO2 natural habitat, O2-stable catalase, and possibly the high glutathione content of the organism explain its aerobic survival in the absence of SOD.

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Neisseria

Tønjum¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Neis.se' ri.a . M.L. fem. n. Neisseria named after Albert Neisser, who discovered the etiological agent of gonorrhea in the pus of patients in 1889. Proteobacteria / Betaproteobacteria / Neisseriales / Neisseriaceae / Neisseria Cocci 0.6–1.9 µm in diameter, occurring singly but often in pairs with adjacent sides flattened; two species ( Neisseria elongata and N . weaveri ) are exceptions and consist of short rods 0.5 µm wide, often arranged as diplobacilli or in chains. Division of the coccal species is in two planes at right angles to each other, sometimes resulting in tetrads. Capsules and fimbriae (pili) may be present. Endospores are not present. Gram negative, but there is a tendency to resist decolorization. Swimming motility does not occur and flagella are absent. Aerobic. Some species produce a yellow carotenoid pigment. Some species are nutritionally fastidious and hemolytic. Optimal temperature, 35–37°C. Oxidase positive. Catalase positive except most strains of N . elongata . Carbonic anhydrase is produced by all species. All species reduce nitrite except N . gonorrhoeae and N . canis . Neisseria spp. produce acid from carbohydrates by oxidation, not fermentation. Chemoorganotrophic. Exotoxins are not produced. Some species are saccharolytic. Inhabitants of the mucous membranes of mammals. Some species are primary pathogens of humans. The genus Neisseria belongs to the family Neisseriaceae of the Betaproteobacteria . The mol % G + C of the DNA is : 48–56. Type species : Neisseria gonorrhoeae (Zopf 1885) Trevisan 1885, 106 ( Merismopedia gonorrhoeae Zopf 1885, 54.)

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Superoxol and amylase inhibition tests for distinguishing gonococcal and nongonococcal cultures growing on selective media

Cited by 13 publications

References 12 publications

Branhamella catarrhalis: an organism gaining respect as a pathogen.

Branhamella catarrhalis: an organism gaining respect as a pathogen.

Superoxide dismutase and oxygen toxicity defenses in the genus Neisseria

Neisseria

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