Superparamagnetic Iron Oxide Nanoparticles Induce Apoptosis in HT-29 Cells by Increasing ROS and Damaging DNA

Ranjbary, Ali Ghorbani; Saleh, Golnaz Karbalaei; Azimi, Mohammadreza

doi:10.21203/rs.3.rs-535697/v1

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…In the present study, we observed inhibition in cleaved caspase-3 activity in rats treated with DEN, which agree with the results reported by Lin et al (2017) [55]. Fe 3 O 4 nanoparticles induce apoptosis in cancer cells by raising the quantity of reactive oxygen species (ROS) and intracellular calcium, as well as boosting the expression of caspase-3 and caspase-9 and decreasing the expression of Bcl-2, as well as causing direct DNA damage [56]. The expression of cleaved caspase-3 is increased by the Fe 3 O 4 /Cs in the current study.…”

Section: Discussionsupporting

confidence: 93%

Antitumor Activity of Chitosan-Coated Iron Oxide Nanocomposite Against Hepatocellular Carcinoma in Animal Models

Badawy

Abdel-Hamid

Mohamed

2022

Biol Trace Elem Res

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Hepatocellular carcinoma (HCC) is among the most prevalent and lethal cancers worldwide. Chitosan-coated iron oxide nanocomposite (Fe3O4/Cs) is a promising bio-nanomaterial for many biological applications. The objective of this research was to evaluate the anticancer efficacy of Fe3O4/Cs against HCC in animal models. Fe3O4 nanoparticles were prepared and added to chitosan solution; then, the mixture was exposed to gamma radiation at a dose of 20 kGy. Rats have received diethylnitrosamine (DEN) orally at a dose of 20 mg/kg body weight 5 times per week during a period of 10 weeks to induce HCC and then have received Fe3O4/Cs intraperitoneal injection at a dose of 50 mg/kg body weight 3 times per week during a period of 4 weeks. After the last dose of Fe3O4/Cs administration, animals were sacrificed. DEN induced upregulation of PI3K/Akt/mTOR and MAPK (ERK, JNK, P38) signaling pathways and inflammatory markers (TLR4, iNOS, and TNF-α). DEN also decreases cleaved caspase-3 and increases liver enzymes (ALT, AST, and GGT) activities. Administration of Fe3O4/Cs significantly ameliorated the above-mentioned parameters.

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Section: Discussionsupporting

confidence: 93%

Antitumor Activity of Chitosan-Coated Iron Oxide Nanocomposite Against Hepatocellular Carcinoma in Animal Models

Badawy

Abdel-Hamid

Mohamed

2022

Biol Trace Elem Res

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“…Epigenetic changes related to DNA methylation patterns and the expression of non-coding RNAs are also a potential effect of both iron and iron oxides. According to some authors, IONPs can also induce generation of reactive oxygen species (ROS) and subsequent oxidative damage to DNA and chromatin structure [31][32][33] . In our study, it is well possible that discrete alterations in chromatin distribution detected through the changes in run length matrix and wavelet parameters were the result of early chromatin condensation or changes in euchromatin / heterochromatin ratio.…”

Section: Discussionmentioning

confidence: 99%

Artificial intelligence strategies based on run length matrix and wavelet analyses for detection of subtle alterations in hepatocyte chromatin organization following exposure to iron oxide nanoparticles

Pantic,

Vucevic,

Radosavljevic

et al. 2024

Preprint

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This study focuses on the development of machine learning models based on the features of the run length matrix (RLM) and wavelet analyses, with the potential to detect subtle alterations in hepatocyte chromatin organization due to iron oxide nanoparticle exposure. A total of 2000 hepatocyte nuclear regions of interest (ROIs) from mouse liver tissue were analyzed, and for each ROI, 5 different parameters were calculated: Long Run Emphasis, Short Run Emphasis, Run Length Nonuniformity, and 2 wavelet coefficient energies obtained after the discrete wavelet transform. These parameters served as input for supervised machine learning models, specifically random forest and gradient boosting classifiers. The models demonstrated robust performance in distinguishing hepatocyte chromatin structures belonging to the group exposed to IONPs from the controls. The study's findings suggest that iron oxide nanoparticles induce substantial changes in hepatocyte chromatin distribution and underscore the potential of AI techniques in advancing hepatocyte evaluation in physiological and pathological conditions.

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Magnetic Nanoparticles: Current Advances in Nanomedicine, Drug Delivery and MRI

Comănescu

2022

Chemistry

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Magnetic nanoparticles (MNPs) have evolved tremendously during recent years, in part due to the rapid expansion of nanotechnology and to their active magnetic core with a high surface-to-volume ratio, while their surface functionalization opened the door to a plethora of drug, gene and bioactive molecule immobilization. Taming the high reactivity of the magnetic core was achieved by various functionalization techniques, producing MNPs tailored for the diagnosis and treatment of cardiovascular or neurological disease, tumors and cancer. Superparamagnetic iron oxide nanoparticles (SPIONs) are established at the core of drug-delivery systems and could act as efficient agents for MFH (magnetic fluid hyperthermia). Depending on the functionalization molecule and intrinsic morphological features, MNPs now cover a broad scope which the current review aims to overview. Considering the exponential expansion of the field, the current review will be limited to roughly the past three years.

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Superparamagnetic Iron Oxide Nanoparticles Induce Apoptosis in HT-29 Cells by Increasing ROS and Damaging DNA

Cited by 3 publications

References 33 publications

Antitumor Activity of Chitosan-Coated Iron Oxide Nanocomposite Against Hepatocellular Carcinoma in Animal Models

Antitumor Activity of Chitosan-Coated Iron Oxide Nanocomposite Against Hepatocellular Carcinoma in Animal Models

Artificial intelligence strategies based on run length matrix and wavelet analyses for detection of subtle alterations in hepatocyte chromatin organization following exposure to iron oxide nanoparticles

Magnetic Nanoparticles: Current Advances in Nanomedicine, Drug Delivery and MRI

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