Superparamagnetic Molecularly Imprinting Polymers for Adsorbent and Separation Pentapeptides by Surface ATRP

Abstract

A technique was used to prepare biomacromolecules imprinting polymers for adsorbent and fast separation and purification of thymopentin (Tp5). In order to overcome intrinsic and keep biomacromolecules structural, surface ATRP was used to prepare magnetic molecularly imprinted polymers (MMIPs) at mild conditions. The interactions between pentapeptides and monomers were studied by molecular dynamics. The ratio of functional monomer MMA to template pentapeptides in ethanol-water was studied by UV-Vis spectrophotometric analysis at pre-polymerization. The Fe 3 O 4 -MIPs were characterized by Fourier transmission infrared spectrometry (FT-IR), scanning electron microscope (SEM), transmission electron microscope (TEM), vibrating sample magnetometer (VSM), thermogravimetry (TGA), and X-ray diffractometer (XRD) analysis. The results demonstrated that Fe 3 O 4 -MIPs possessed high isolation and identification of performance to the Tp5.

The Use of Computational Methods for the Development of Molecularly Imprinted Polymers

“…This approach has since been adapted several times in the literature [ 329 , 330 , 331 , 332 , 333 , 334 , 335 , 336 , 337 , 338 , 339 , 340 , 341 , 342 , 343 , 344 , 345 , 346 , 347 , 348 , 349 , 350 , 351 , 352 , 353 ]. In a number of reports, similar approaches have been employed to evaluate and/or characterize monomer–template interactions using MD and docking simulations as well as variations and/or combinations thereof [ 102 , 354 , 355 , 356 , 357 , 358 , 359 , 360 , 361 , 362 , 363 , 364 , 365 , 366 , 367 , 368 , 369 , 370 , 371 , 372 , 373 , 374 , 375 , 376 , 377 ,…”