Superparamagnetic Nanosystems Based on Iron Oxide Nanoparticles for Biomedical Imaging

Liu, Fujun; Laurent, Sophie; Fattahi, Hassan; Elst, Luce Vander; Müller, Robert N.

doi:10.2217/nnm.11.16

Cited by 82 publications

(41 citation statements)

References 99 publications

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“…37,38 Spherical SPIONs with average core diameters between 10 and 25 nm were studied (TEM images shown in Supporting Information Figures S2 and S8). These NPs have comparable dimensions as proteins and are able to escape from opsonization and the reticuloendothelial system.…”

Section: Resultsmentioning

confidence: 99%

Size and Surface Functionalization of Iron Oxide Nanoparticles Influence the Composition and Dynamic Nature of Their Protein Corona

Ashby

Pan

Zhong

2014

ACS Appl. Mater. Interfaces

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Nanoparticles (NPs) adsorb proteins when in the biological matrix, and the resulted protein corona could affect NP-cell interactions. The corona has a dynamic nature with the adsorbed proteins constantly exchanging with the free proteins in the matrix at various rates. The rapidly exchanging proteins compose the soft corona, which responds more dynamically to environment changes than the hard corona established by the ones with slow exchange rates. In the present study, the corona formed on the superparamagnetic iron oxide NPs (SPIONs) in human serum was studied by flow field-flow fractionation and ultracentrifugation, which rapidly differentiated the corona proteins based on their exchange rates. By varying the surface hydrophobicity of the SPIONs with a core size around 10 nm, we found out that, the more hydrophobic surface ligand attracted proteins with higher surface hydrophobicity and formed a more dynamic corona with a larger portion of the involved proteins with fast exchange rates. Increasing the core diameter of the SPIONs but keeping the surface ligand the same could also result in a more dynamic corona. A brief investigation of the effect on the cellular uptake of SPIONs using one selected corona protein, transferrin, was conducted. The result showed that, only the stably bound transferrin could significantly enhance cellular uptake, while transferrin bound in a dynamic nature had negligible impact. Our study has led to a better understanding of the relationship between the particle properties and the dynamic nature of the corona, which can help with design of nanomaterials with higher biocompatibility and higher efficacy in biosystems for biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Size and Surface Functionalization of Iron Oxide Nanoparticles Influence the Composition and Dynamic Nature of Their Protein Corona

Ashby

Pan

Zhong

2014

ACS Appl. Mater. Interfaces

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“…Nanoparticles have gained an increasing importance as promising new tools for drug targeting, hyperthermia and imaging. [6][7][8][9][10][11] Especially, biocompatible superparamagnetic iron oxide nanoparticles (SPIONs), which have been in clinical…”

Section: Introductionmentioning

confidence: 99%

Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

Poller¹,

Zaloga²,

Schreiber³

et al. 2017

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) are promising tools for the treatment of different diseases. Their magnetic properties enable therapies involving magnetic drug targeting (MDT), hyperthermia or imaging. Depending on the intended treatment, specific characteristics of SPIONs are required. While particles used for imaging should circulate for extended periods of time in the vascular system, SPIONs intended for MDT or hyperthermia should be accumulated in the target area to come into close proximity of, or to be incorporated into, specific tumor cells. In this study, we determined the impact of several accurately characterized SPION types varying in size, zeta potential and surface coating on various human breast cancer cell lines and endothelial cells to identify the most suitable particle for future breast cancer therapy. We analyzed cellular SPION uptake, magnetic properties, cell proliferation and toxicity using atomic emission spectroscopy, magnetic susceptometry, flow cytometry and microscopy. The results demonstrated that treatment with dextran-coated SPIONs (SPION Dex ) and lauric acid-coated SPIONs (SPION LA ) with an additional protein corona formed by human serum albumin (SPION LA-HSA ) resulted in very moderate particle uptake and low cytotoxicity, whereas SPION LA had in part much stronger effects on cellular uptake and cellular toxicity. In summary, our data show significant dose-dependent and particle type-related response differences between various breast cancer and endothelial cells, indicating the utility of these particle types for distinct medical applications.

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“…Among the most commonly used nano CAs, superparamagnetic iron oxide nanoparticles (SPIONs) are of particular interest for their good biocompatibility, superior magnetic resonance (MR) T 2 (transverse relaxation) shortening effects, and biodegradability. [3][4][5][6] Furthermore, SPIONs exhibit large surface areas, making them favorable for versatile surface functionalization and conjugation of biomolecules. 7,8 Two most extensively used methods in preparing SPIONs for magnetic resonance imaging (MRI) are coprecipitation and thermal decomposition.…”

Section: Introductionmentioning

confidence: 99%

Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells

Wang¹,

Xing²,

Zhang³

et al. 2014

IJN

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Targeted imaging contrast agents for early pancreatic ductal adenocarcinoma diagnosis was developed using superparamagnetic iron oxide nanoparticles (SPIONs). For phase transfer of SPIONs, the hydrophobic SPIONs are first treated with tetrafluoroborate and then capped by bovine serum albumin (BSA) via ligand exchange. It was experimentally found that nitrosyl tetrafluoroborate pretreatment and proper structures of molecules are essential to the effective surface functionalization of SPIONs. Nonspecific binding was found to be significantly reduced by BSA surface functionalized hydrophobic SPIONs (BSA·SPIONs). The BSA·SPIONs were monodispersed with an average size of approximately 18.0 nm and stable in a wide pH range and various ionic strengths even after 7 days of storage. The longitudinal and transverse proton relaxation rate (r 1 , r 2 ) values of the BSA·SPIONs were determined to be 11.6 and 154.2 s −1 per mM of Fe 3+ respectively. The r 2 /r 1 ratio of 13.3 ensured its application as the T 2 -weighted magnetic resonance imaging contrast agents. When conjugated with near-infrared fluorescent dye and monoclonal antibody, the dye BSA·SPION-monoclonal antibody bioconjugates showed excellent targeting capability with minimal nonspecific binding in the bimodal imaging of pancreatic cancer cells. The experimental approach is facile, environmentally benign, and straightforward, which presents great promise in early cancer diagnosis.

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Superparamagnetic Nanosystems Based on Iron Oxide Nanoparticles for Biomedical Imaging

Cited by 82 publications

References 99 publications

Size and Surface Functionalization of Iron Oxide Nanoparticles Influence the Composition and Dynamic Nature of Their Protein Corona

Size and Surface Functionalization of Iron Oxide Nanoparticles Influence the Composition and Dynamic Nature of Their Protein Corona

Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells

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