Superresolution Imaging of Photochromic Acylhydrazone Moieties on Amyloid Nanofibrils: Implications for Photoswitchable Probes

Chen, Bryan Po-Wen; He, Rong; Chien, Hung-Ming; Lee, Chi-Chang; Chuang, Hsiao-Han; Hsu, Chao‐Ping; Chan, Jerry C. C.; Huang, Joseph Jen‐Tse

doi:10.1021/acsanm.1c04004

“…A spiropyran scaffold was used to develop the β‐galactosidase‐responsive sensor NpG (Figure 16d), which is used in STORM imaging in various cancer cell lines [146] . Acylhydrazones have also been reported in photochromic switches [147] . In combination with peptide targeting groups, this class of photochromic switch was used in dSTORM and PALM imaging of amyloid nanofibrils [147] …”

Section: Photochemical Mechanisms Of Fluorophores Used In Smlmmentioning

confidence: 99%

“…Acylhydrazones have also been reported in photochromic switches [147] . In combination with peptide targeting groups, this class of photochromic switch was used in dSTORM and PALM imaging of amyloid nanofibrils [147] …”

Section: Photochemical Mechanisms Of Fluorophores Used In Smlmmentioning

confidence: 99%

Photochemical Mechanisms of Fluorophores Employed in Single‐Molecule Localization Microscopy

Kikuchi

¹

,

Adair

²

,

Lin

³

et al. 2022

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Decoding cellular processes requires visualization of the spatial distribution and dynamic interactions of biomolecules. It is therefore not surprising that innovations in imaging technologies have facilitated advances in biomedical research. The advent of super‐resolution imaging technologies has empowered biomedical researchers with the ability to answer long‐standing questions about cellular processes at an entirely new level. Fluorescent probes greatly enhance the specificity and resolution of super‐resolution imaging experiments. Here, we introduce key super‐resolution imaging technologies, with a brief discussion on single‐molecule localization microscopy (SMLM). We evaluate the chemistry and photochemical mechanisms of fluorescent probes employed in SMLM. This Review provides guidance on the identification and adoption of fluorescent probes in single molecule localization microscopy to inspire the design of next‐generation fluorescent probes amenable to single‐molecule imaging.

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“…Photoswitchable small molecules and fluorescent proteins are paving the way for cutting-edge technologies, including optogenetics, , photopharmacology, − optical information processing, − energy conversion, , super-resolution microscopy, − and 3D printing . Among different photochromic and photoswitchable compounds, hemithioindigos (HTIs) are highly promising for chemical and biological applications due to their properties resulting from the geometric change .…”

mentioning

confidence: 99%

Proton-Coupled Photochromic Hemithioindigo: Toward Photoactivated Chemical Sensing and Imaging

Li

¹

,

Ma

²

,

Wang

³

et al. 2023

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We report photoswitchable fluorescent hemithioindigos (HTIs) where the metastable E isomers were stabilized by the proton-bridged intramolecular hydrogen bond. Titration experiments and computational analysis indicated that the E isomers were much more basic than the Z isomers, which enabled photoactivated colorimetric and fluorescent pH response in solvents and polypropylene films. The HTIs exhibited reversibly switchable fluorescence with the Z isomers being the most fluorescent. Moreover, the HTIs were lysosomotropic and the kinetic fluorescence evolution during photoswitching was able to differentiate subcellular compartments with different pH. The combination of photoenhanced basicity, switchable fluorescence, and proton-coupled photochromism lay the groundwork for a broad range of chemical and biological applications.

show abstract

Photochemical Mechanisms of Fluorophores Employed in Single‐Molecule Localization Microscopy

Kikuchi

¹

,

Adair

²

,

Lin

³

et al. 2022

View full text Add to dashboard Cite

Decoding cellular processes requires visualization of the spatial distribution and dynamic interactions of biomolecules. It is therefore not surprising that innovations in imaging technologies have facilitated advances in biomedical research. The advent of super‐resolution imaging technologies has empowered biomedical researchers with the ability to answer long‐standing questions about cellular processes at an entirely new level. Fluorescent probes greatly enhance the specificity and resolution of super‐resolution imaging experiments. Here, we introduce key super‐resolution imaging technologies, with a brief discussion on single‐molecule localization microscopy (SMLM). We evaluate the chemistry and photochemical mechanisms of fluorescent probes employed in SMLM. This Review provides guidance on the identification and adoption of fluorescent probes in single molecule localization microscopy to inspire the design of next‐generation fluorescent probes amenable to single‐molecule imaging.

show abstract

Superresolution Imaging of Photochromic Acylhydrazone Moieties on Amyloid Nanofibrils: Implications for Photoswitchable Probes

Cited by 5 publications

References 28 publications

Photochemical Mechanisms of Fluorophores Employed in Single‐Molecule Localization Microscopy

Photochemical Mechanisms of Fluorophores Employed in Single‐Molecule Localization Microscopy

Proton-Coupled Photochromic Hemithioindigo: Toward Photoactivated Chemical Sensing and Imaging

Photochemical Mechanisms of Fluorophores Employed in Single‐Molecule Localization Microscopy

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