Supersensitive photon upconversion based immunoassay for detection of cardiac troponin I in human plasma

Raiko, Kirsti; Lyytikäinen, Annika; Ekman, Miikka; Nokelainen, Aleksi; Lahtinen, Satu; Soukka, Tero

doi:10.1016/j.cca.2021.10.023

Cited by 15 publications

(11 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using a combination of energy migration and core–shell structural engineering to improve properties for a wide range of activators would widen the range of applications for lanthanide-doped nanoparticles [ 53 , 54 , 55 ]. Surface modification is required for specific nanomaterials to fulfil various biological activities, in biosciences such as disease therapy (particularly cancer) [ 56 , 57 ], detection [ 58 ], and immunoassay [ 59 ]. Understanding surface functionalization of Ln-doped UCNPs is critical for improving UC efficiency and aqueous solubility [ 60 ].…”

Section: Surface Modification Of Ucnpsmentioning

confidence: 99%

Lanthanide-Doped Upconversion Luminescent Nanoparticles—Evolving Role in Bioimaging, Biosensing, and Drug Delivery

et al. 2022

View full text Add to dashboard Cite

Upconverting luminescent nanoparticles (UCNPs) are “new generation fluorophores” with an evolving landscape of applications in diverse industries, especially life sciences and healthcare. The anti-Stokes emission accompanied by long luminescence lifetimes, multiple absorptions, emission bands, and good photostability, enables background-free and multiplexed detection in deep tissues for enhanced imaging contrast. Their properties such as high color purity, high resistance to photobleaching, less photodamage to biological samples, attractive physical and chemical stability, and low toxicity are affected by the chemical composition; nanoparticle crystal structure, size, shape and the route; reagents; and procedure used in their synthesis. A wide range of hosts and lanthanide ion (Ln3+) types have been used to control the luminescent properties of nanosystems. By modification of these properties, the performance of UCNPs can be designed for anticipated end-use applications such as photodynamic therapy (PDT), high-resolution displays, bioimaging, biosensors, and drug delivery. The application landscape of inorganic nanomaterials in biological environments can be expanded by bridging the gap between nanoparticles and biomolecules via surface modifications and appropriate functionalization. This review highlights the synthesis, surface modification, and biomedical applications of UCNPs, such as bioimaging and drug delivery, and presents the scope and future perspective on Ln-doped UCNPs in biomedical applications.

show abstract

Section: Surface Modification Of Ucnpsmentioning

confidence: 99%

Lanthanide-Doped Upconversion Luminescent Nanoparticles—Evolving Role in Bioimaging, Biosensing, and Drug Delivery

et al. 2022

View full text Add to dashboard Cite

show abstract

“…This unique anti-Stokes shift property enables its widespread application in the field of biomedicine by avoiding autofluorescence caused by UV-visible excitation in biological samples 23,24 Other key characteristics of the UCNPs are high photostability, deep tissue penetration, chemical stability, large anti-Stokes shift, and a narrow emission peak. 25 Sirkka et al 26 and Raiko et al 27 have fabricated NaYF 4 :Yb,Er based heterogenous UC immunoassays immobilized in streptavidin coated microwells by a biotinylated anti-cTnI monoclonal antibody and a microplate based NaYF 4 :Yb,Er UCNPs sandwich immunoassay for the detection of the cTnI. 26 There are challenges with these immunoassays because of aggregation due to reduction, and non-specific binding interactions with the antibody which necessitates careful optimization conditions for specific detection of cTnI.…”

Section: Introductionmentioning

confidence: 99%

“…26 There are challenges with these immunoassays because of aggregation due to reduction, and non-specific binding interactions with the antibody which necessitates careful optimization conditions for specific detection of cTnI. 27 In 2021, Bayoumy et al developed a NaYF 4 :Yb, Er UCNP based lateral flow immunoassay (LFIA) for the detection of cTnI in human blood. 28 However, the matrix effect, stability, sensitivity and quantitative detection are major limiting factors of LFIA.…”

Section: Introductionmentioning

confidence: 99%

NaYF₄:Yb/Ho upconversion nanoprobe incorporated gold nanoparticle (AuNP) based FRET immunosensor for the “turn-on” detection of cardiac troponin I

Abraham,

Madanan,

Varghese

et al. 2024

Analyst

View full text Add to dashboard Cite

show abstract

“…This is because they are cost effective and are simple tests which can be used with minimal training. In recent years, LFIAs have been reported for measurement of cardiac troponin I using various signal labels such as dual gold nanoparticles 15 , fluorescent microspheres 16 , quantum dots 17 , magnetic particles 18 – 20 , enzyme-catalyzed labeling 21 , 22 , upconverting nanoparticles 23 , 24 , and ion-doped nanoparticles 25 . However, these assays have their own limitations.…”

Section: Introductionmentioning

confidence: 99%

Silver-enhanced colloidal gold dip strip immunoassay integrated with smartphone-based colorimetry for sensitive detection of cardiac marker troponin I

Poosinuntakul

Chanmee

Porntadavity

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Cardiac troponin I (cTnI) is a specific cardiac biomarker for diagnosis of acute myocardial infarction (AMI). A sensitive and simple point-of-care test (POCT) is still required for early detection of AMI. To address this need, we developed a dip strip assay based on sandwich immunoassay coupled with a silver enhancement system. Pre-incubation and silver enhancement were introduced to the dip strip to increase sensitivity. Due to the catalytic reaction of the silver enhancement solution, the red color of AuNPs changed to dark brown as silver ions precipitated and enlarged the AuNPs. The obtained results were easily seen by the naked eye. For quantitative analysis, the color intensity of the results was analyzed using a smartphone with RGB color picker application. The effects of operating parameters (volume of AuNP-Ab conjugate, volume of sample, incubation time, and analysis time) were investigated and optimized. Under optimal conditions, the limit of detection (LOD) by the naked eye was 0.5 ng/mL. The LOD with silver enhancement was 50-fold lower than without. For quantitative analysis using the smartphone, linearity of detection was observed through the range of 0.5–50 ng/mL (R2 = 0.9952) and the LOD was 0.12 ng/mL. The developed method was successfully applied to detection of cTnI in serum samples, achieving analytical recoveries and %RSD in the ranges of 96.10–119.17% and 2.91–5.13%, respectively. Additionally, this developed assay was not cross reactive with the potentially interfering serum proteins. These results showed the great potential of this dip strip assay as an alternative POCT for detection of serum cTnI.

show abstract

Supersensitive photon upconversion based immunoassay for detection of cardiac troponin I in human plasma

Cited by 15 publications

References 22 publications

Lanthanide-Doped Upconversion Luminescent Nanoparticles—Evolving Role in Bioimaging, Biosensing, and Drug Delivery

Lanthanide-Doped Upconversion Luminescent Nanoparticles—Evolving Role in Bioimaging, Biosensing, and Drug Delivery

NaYF₄:Yb/Ho upconversion nanoprobe incorporated gold nanoparticle (AuNP) based FRET immunosensor for the “turn-on” detection of cardiac troponin I

Silver-enhanced colloidal gold dip strip immunoassay integrated with smartphone-based colorimetry for sensitive detection of cardiac marker troponin I

Contact Info

Product

Resources

About

Supersensitive photon upconversion based immunoassay for detection of cardiac troponin I in human plasma

Cited by 15 publications

References 22 publications

Lanthanide-Doped Upconversion Luminescent Nanoparticles—Evolving Role in Bioimaging, Biosensing, and Drug Delivery

Lanthanide-Doped Upconversion Luminescent Nanoparticles—Evolving Role in Bioimaging, Biosensing, and Drug Delivery

NaYF4:Yb/Ho upconversion nanoprobe incorporated gold nanoparticle (AuNP) based FRET immunosensor for the “turn-on” detection of cardiac troponin I

Silver-enhanced colloidal gold dip strip immunoassay integrated with smartphone-based colorimetry for sensitive detection of cardiac marker troponin I

Contact Info

Product

Resources

About

NaYF₄:Yb/Ho upconversion nanoprobe incorporated gold nanoparticle (AuNP) based FRET immunosensor for the “turn-on” detection of cardiac troponin I