Superstimulation of ovarian follicular development in beef cattle with a single intramuscular injection of Folltropin-V

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

“…At the site of the combined HA-agent injection, body temperature slowly broke the HA structure in each layer and, thus, caused its release into blood circulation (Cascone et al 1994;Luo et al 2000). In cattle, HA was successfully used for superovulation by single injection with slow FSH release (Tribulo et al 2011). However, in this preliminary study, in which we used a single FSH injection for superovulation, the ovarian response was poorer than that of a traditional FSH multiple injection protocol.…”

“…injection of Folltropin ® -V dissolved in HA (HA-FSH) and traditional twicedaily i.m. injections (Tribulo et al 2011;Tribulo et al 2012).…”

<b>Suplementação exógena com gonadotrofinas para aumentar a produção <i>in vitro</i> de embriões em doadoras Holandesas</b>

“…Previous studies reported a similar number of transferable embryos when a single (in 2% HA) (TRÍBULO et al, 2011) or two (in 0.5 or 1% HA) (TRÍBULO et al, 2012) IM injections of pFSH was administered compared to the traditional twice-daily IM injection protocol. The same authors emphasized that the need for the second injection is related to the concentration of HA; the lower concentrations are less viscous and easier to mix with pFSH, but are not as efficacious in sustaining circulating FSH levels (TRÍBULO et al, 2011(TRÍBULO et al, , 2012.…”

“…The same authors emphasized that the need for the second injection is related to the concentration of HA; the lower concentrations are less viscous and easier to mix with pFSH, but are not as efficacious in sustaining circulating FSH levels (TRÍBULO et al, 2011(TRÍBULO et al, , 2012. Therefore, considering the shorter period of FSH support required for superstimulation prior to OPU (BLONDIN et al, 2002;VIEIRA et al, 2014a) compared to traditional multiple ovulation and in vivo embryo procedures [reviewed by Mapletoft and Bó (2012)], the use of a single injection of pFSH in 0.5% HA (5 mg/mL) might be an alternative to simplify the superstimulatory protocol for OPU-IVP programs.…”

“…An alternative that has been studied for in vivo embryo production is the use of a single injection of pFSH in a hyaluronan (HA) solution (TRÍBULO et al, 2011). The HA is a biodegradable polymer that apparently results in a sustainable slow release of hormones.…”