Superstimulation with eCG prior to ovum pick-up improves follicular development and fertilization rate of cattle oocytes

Ribas, Bibiana Noal; Míssio, Daniele; Roman, Isac Junior; Neto, Normélio Alves; Claro, I.; Brum, Daniela dos Santos; Leivas, Fábio Gallas

doi:10.1016/j.anireprosci.2018.06.006

Cited by 11 publications

(8 citation statements)

References 25 publications

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“…The most relevant finding was that, despite the increased blastocyst yields, the mean number of embryos produced per donor per session was similar to that recorded in non-eCG-treated cows. This is certainly due to the reduced recovery rate in eCG-treated group, that was also previously reported (Pieterse 1992;Ribas et al 2018), likely due to the higher incidence of medium size follicles (Seneda et al 2001). It has been speculated that the larger volume, associated with increased viscosity and higher intra-follicular pressure of larger follicles may in part interfere with oocyte collection during the insertion and rotation of the needle inside the follicle.…”

Section: Discussionmentioning

confidence: 70%

Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study

Presicce

Neglia

Salzano

et al. 2019

Italian Journal of Animal Science

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The study evaluated the effects of eCG treatment prior to ovum pick up (OPU) on follicular population, oocyte and embryo yields in summer and autumn in Podolic cattle. The effects of repeated OPU on cattle wellbeing was also documented. Twenty-six animals were used, and split into two groups, treatment (OPU; n ¼ 18) and control (CG; n ¼ 8). The OPU cattle were subsequently split into two subgroups (n ¼ 9) and underwent repeated OPU, without and with eCG priming, for a total of 8 sessions (4 sessions/season). Follicular population, oocyte and embryo yields were recorded in those subgroups. CG was handled in the same manner of OPU, except for epidural anaesthesia and follicular aspiration. Biochemical profile, serum protein electrophoresis and haptoglobin levels were analysed in OPU and CG. Hormonal priming increased the number of medium follicles (1.7 ± 0.2 vs 0.9 ± 0.2, p < .05), while it decreased the recovery rate and number of cumulus-enclosed oocytes (COCs) (recovery rate: 38.3 ± 3.5 vs 60.5 ± 4.0%; COCs: 2.3 ± 0.3 vs 3.4 ± 0.4, respectively; p < .01). However, priming increased cleavage (72.9 ± 5.7 vs 49.4 ± 5.4; p < .05) and blastocyst (41.1 ± 5.7 vs 23.0 ± 4.2; p ¼ .054) rates. With regard to season's effect, a higher number of COCs was recorded in autumn than in summer (3.1 ± 0.4 vs 2.6 ± 0.3; p < .05) without affecting though the number of embryos produced (0.9 on average). Since haematological parameters did not vary between OPU and CG, our preliminary data suggest that repeated OPU may be used as a conservation strategy in Podolic cattle without affecting wellbeing. HIGHLIGHTS Podolic cattle is an endangered Italian breed, reared in semi-extensive/extensive systems Ovum pickup (OPU) can be carried out in this breed without impairing animal health and welfare The eCG treatment before OPU (priming) did not improve the number of embryos produced per donor.

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Section: Discussionmentioning

confidence: 70%

Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study

Presicce

Neglia

Salzano

et al. 2019

Italian Journal of Animal Science

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“…The majority of GW mosaic bovine embryos described here contain two different paternal haplotypes indicating the oocyte was fertilized by (at least) two sperm, pointing to polyspermic fertilization as a major trigger of multipolar and heterogoneic division of the zygote. Polyspermy occurs at similar frequencies for human (6–32% [ 93 , 94 ];) and bovine (15–35% [ 95 , 96 ];) in in vitro-fertilized zygotes. Yet, in human reproductive medicine, polyspermy is avoided by the use of ICSI (76% of 2019 cycles in Belgium) and the selection against zygotes presenting with more than two pronuclei at day 1.…”

Section: Discussionmentioning

confidence: 99%

Parental genomes segregate into distinct blastomeres during multipolar zygotic divisions leading to mixoploid and chimeric blastocysts

Coster

Masset²,

Tšuiko³

et al. 2022

Genome Biol

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Background During normal zygotic division, two haploid parental genomes replicate, unite and segregate into two biparental diploid blastomeres. Results Contrary to this fundamental biological tenet, we demonstrate here that parental genomes can segregate to distinct blastomeres during the zygotic division resulting in haploid or uniparental diploid and polyploid cells, a phenomenon coined heterogoneic division. By mapping the genomic landscape of 82 blastomeres from 25 bovine zygotes, we show that multipolar zygotic division is a tell-tale of whole-genome segregation errors. Based on the haplotypes and live-imaging of zygotic divisions, we demonstrate that various combinations of androgenetic, gynogenetic, diploid, and polyploid blastomeres arise via distinct parental genome segregation errors including the formation of additional paternal, private parental, or tripolar spindles, or by extrusion of paternal genomes. Hence, we provide evidence that private parental spindles, if failing to congress before anaphase, can lead to whole-genome segregation errors. In addition, anuclear blastomeres are common, indicating that cytokinesis can be uncoupled from karyokinesis. Dissociation of blastocyst-stage embryos further demonstrates that whole-genome segregation errors might lead to mixoploid or chimeric development in both human and cow. Yet, following multipolar zygotic division, fewer embryos reach the blastocyst stage and diploidization occurs frequently indicating that alternatively, blastomeres with genome-wide errors resulting from whole-genome segregation errors can be selected against or contribute to embryonic arrest. Conclusions Heterogoneic zygotic division provides an overarching paradigm for the development of mixoploid and chimeric individuals and moles and can be an important cause of embryonic and fetal arrest following natural conception or IVF.

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“…Exocytosis of CGs is very important in the prevention of polyspermy [26, 34]. A recent study showed that oocytes collected from cows superstimulated prior to OPU had a lower rate of polyspermy than oocytes collected from non-stimulated cows [35]. Thus, this finding in combination with our findings suggests that normal CGs distribution during cytoplasmic maturation of in vivo -matured oocytes could efficiently prevent polyspermy occurrence, whereas polyspermy would frequently occur in vitro -matured oocytes due to the abnormal distribution of their CGs.…”

Section: Discussionmentioning

confidence: 99%

Efficient <i>in vitro</i> embryo production using <i>in vivo</i>-matured oocytes from superstimulated Japanese Black cows

et al. 2019

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We examined whether the use of in vivo -matured oocytes, collected by ovum pick-up (OPU) from superstimulated Japanese Black cows, can improve the productivity and quality of in vitro produced embryos. The cows were superstimulated by treatment with progesterone, GnRH, FSH and prostaglandin F2α according to a standardized protocol. The resulting in vivo -matured oocytes were collected by OPU and used subsequently for the other experiments. The immature oocytes from cows in the non-stimulated group were collected by OPU and then subjected to maturation in vitro . We found that the rate of normally distributed cortical granules of the matured oocyte cytoplasm in the superstimulated group was significantly higher than that in the non-stimulated group. The normal cleavage rate (i.e., production of embryos with two equal blastomeres without fragmentation) and freezable blastocyst rate were significantly higher in the superstimulated group than in the non-stimulated group. Among the transferable blastocysts, the ratio of embryos from normal cleavage was also significantly higher in the superstimulated group than in the non-stimulated group. For in vivo -matured oocytes, it was observed that the pregnancy rates were significantly higher when normally cleaved embryos were used for transfer. Taken together, these results suggest that high-quality embryos with respect to developmental kinetics can be efficiently produced with the use of in vivo -matured oocytes collected by OPU from superstimulated Japanese Black cows.

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Superstimulation with eCG prior to ovum pick-up improves follicular development and fertilization rate of cattle oocytes

Cited by 11 publications

References 25 publications

Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study

Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study

Parental genomes segregate into distinct blastomeres during multipolar zygotic divisions leading to mixoploid and chimeric blastocysts

Efficient <i>in vitro</i> embryo production using <i>in vivo</i>-matured oocytes from superstimulated Japanese Black cows

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