Supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in India

Chakraborty, Supriya; Ramachandran, Vanitharani; Chattopadhyay, B.; Fauquet, Claude

doi:10.1099/vir.0.82873-0

Cited by 94 publications

(63 citation statements)

References 38 publications

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“…The sequences produced as part of this study are in the shaded square viruses share same vector (whitefly) and thus the chance of the transmission of co-existing viruses increases, as the synergism among the virus species increases the amount of both viruses in the systemically infected leaves. As a result, crops which are infected by more than one virus species or by mixed infection, have a potential source of inoculum for both viruses, and whiteflies (Chakraborty et al, 2008). Our findings here have shown that radish in Oman is such a host that can harbor two viruses and thus can act as a reservoir of viruses.…”

Section: Discussionmentioning

confidence: 68%

“…A mixed infection potentially leads to geminivirus variability Zhou et al, 1997;Fondong et al, 2000). Mixed infections also can affect symptoms, and virus accumulation, because they often result in synergistic interactions (Chakraborty et al, 2008;Alves-Júnior et al, 2009). The mechanism of the synergistic interaction of geminiviruses is not fully understood.…”

Section: Introductionmentioning

confidence: 99%

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Association of Tomato Yellow Leaf Curl Virus and Chili Leaf Curl Virus with Leaf Curl Disease of Radish and the Synergistic Interaction on Nicotiana benthamiana

Al-Shihi

Ammara

Amin

et al. 2017

IJAB

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Leaf yellowing and rolling with slight crinkling was observed in radish plants in Oman, indicative of a begomovirus infection. Viral sequences from radish were 99% identical to sequences of Tomato yellow leaf curl virus (TYLCV) and Chilli leaf curl virus (ChiLCV), from Oman. No beta-or alpha-satellites were detected. Infectious clones were developed and infectivity analyses of isolated viruses were done in a model host Nicotiana benthamiana. Both viruses, when inoculated separately, produced typical symptoms; however, symptom severity increased when these viruses were co-inoculated. Southern analyses confirmed that virus accumulation was high when both viruses were inoculated as compared to independent inoculation. Realtime qPCR analyses showed that copy number of both viruses was 1 X 10 6 copies when N. benthamiana plants were inoculated with either TYLCV or ChiLCV; however, the copy number increased to 1 X 10 8 copies when the two viruses were co-inoculated together in N. benthamiana.

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Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Association of Tomato Yellow Leaf Curl Virus and Chili Leaf Curl Virus with Leaf Curl Disease of Radish and the Synergistic Interaction on Nicotiana benthamiana

Al-Shihi

Ammara

Amin

et al. 2017

IJAB

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“…Among the different viruses infecting tomato, leaf curl causing geminiviruses and Cucumber mosaic virus (CMV) are the two major viruses imposing significant threat to successful cultivation of this crop worldwide. During mixed infections, the synergism might result in the exacerbation of disease symptoms, the increase of virus titer, and the complementation of movement defects due to host restrictions, so that either of them can spread systemically and accumulate at higher level (García-Cano et al 2006;Chakraborty et al 2008;Rentería-Canett et al 2011). Geminiviruses are ssDNA viruses causing leaf curl disease in several crop plants including chilli, okra, cotton, cassava, radish, cabbage etc.…”

Section: Introductionmentioning

confidence: 99%

“…Geminiviruses are ssDNA viruses causing leaf curl disease in several crop plants including chilli, okra, cotton, cassava, radish, cabbage etc. (Moffat 1999;Boulton 2003;Mansoor et al 2006;Chakraborty et al 2008;Singh et al 2012). During the last decade, geminiviruses have emerged as the most notorious plant pathogens causing severe crop loss worldwide.…”

Section: Introductionmentioning

confidence: 99%

Identification of siRNA generating hot spots in multiple viral suppressors to generate broad-spectrum antiviral resistance in plants

Sharma

Kushwaha

Basu

et al. 2014

Physiol Mol Biol Plants

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Viruses are one of the most devastating plant pathogens causing severe economic losses worldwide. RNA silencing is a robust technology to knock down the expression of specific genes. This mechanism can be exploited to generate virus resistant plants through expression of the viral derived sequences. Viruses in turn have evolved to encode suppressors of RNA silencing to combat host defense. Mixed infection of plants is of common occurrence in nature and simultaneous targeting of suppressor(s) of multiple viruses offers an effective strategy. In this study, we have in silico designed siRNAs against suppressors of the two most devastating viruses of tomato, leaf curl causing tomato begomoviruses and Cucumber mosaic virus. Three different siRNA prediction programs were used to evaluate siRNAs generating capability of each sequence and common putative candidate siRNAs were selected fulfilling the stringent parameters. Our results indicated that in the case of each suppressor a particular region of 100-150 base pairs could be source of potent siRNAs referred as hotspots. Expression of these viral hot spots as a single construct in the plants would facilitate development of transgenic plants with a high degree of broad spectrum resistance against multiple viruses.

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“…Mixed infections are common in the field, favoring recombination and pseudorecombination and facilitating the emergence of new strains or species better adapted to new hosts (Chakraborty et al 2008;Davino et al 2009;Patil & Fauquet 2009;Pita et al 2001).…”

mentioning

confidence: 99%

MOLECULAR ANALYSIS OF THE DNA-A OF THE BEGOMOVIRUS TOMATO MOTTLE LEAF CURL VIRUS (ToMoLCV)

Rocha¹,

Xavier²,

Lima³

et al. 2011

VR&R

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Begomoviruses cause economic losses in many crops, mainly in tropical and subtropical regions. Their genome is formed by one or two components and are transmitted by the whitefly Bemisia tabaci to dicotyledonous plants. In Brazil, a viral complex comprised of at least eight species is responsible for severe losses in tomato crops. Tomato and weed samples were collected in tomato-growing regions of the state of Minas Gerais, in southeastern Brazil in 2008 and 2010. Previously described viruses were prevalent in the samples. Two isolates of the partially sequenced Tomato mottle leaf curl virus (ToMoLCV) were associated with tomato plants collected in the city of Jaíba in northern Minas Gerais. Here, we describe the virus's complete DNA-A sequence and its molecular characterization. Genome analysis indicates that the ToMoLCV is a typical New World bipartite begomovirus with greater sequence identity with begomoviruses from Brazil and Central America. Phylogenetic analysis confirms that ToMoLCV clusters with New World begomoviruses from Brazil and Central America. Like all Brazilian begomoviruses, ToMoLCV has a recombinant nature. Together, these results support the classification of ToMoLCV as a distinct species in the genus Begomovirus.

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Supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in India

Cited by 94 publications

References 38 publications

Association of Tomato Yellow Leaf Curl Virus and Chili Leaf Curl Virus with Leaf Curl Disease of Radish and the Synergistic Interaction on Nicotiana benthamiana

Association of Tomato Yellow Leaf Curl Virus and Chili Leaf Curl Virus with Leaf Curl Disease of Radish and the Synergistic Interaction on Nicotiana benthamiana

Identification of siRNA generating hot spots in multiple viral suppressors to generate broad-spectrum antiviral resistance in plants

MOLECULAR ANALYSIS OF THE DNA-A OF THE BEGOMOVIRUS TOMATO MOTTLE LEAF CURL VIRUS (ToMoLCV)

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